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SPT-PTM-POOL-Rme-2

SpikeMix™ PTM-Kit 29 - Arg(Me)

US$1,768.00

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About SpikeMix™ PTM-Kit 29 - Arg(Me)

Pool of proteotypic Arg(Me)- containing peptides.

SpikeMix™ PTM-Kit 29 - Arg(Me) - Specifications

  • Peptide sequence: See accompanying Excel file
  • Amount: 100 pmol/peptide
  • Purity: crude
  • Delivery Format: Freeze-dried in plastic vial
  • Application(s): Proteomics
  • Condition(s)/Topic(s): PRM Assay, Control, PTM Proteomics, SRM Assay
  • Standard Delivery Time: 2-5 days

Are you interested in your other peptides or modifications? Choose your sequence, amount, modification and purity. We will assist you along the way! Custom Peptide Synthesis.

SpikeMix™ Non-quantified PTM Reference Kits
Despite recent improvements, PTM proteomics is still challenging. Typical problems are low endogenous abundance, low ionization intensity, changed fragmentation, limited stability during proteomics workflows, and/or complex fragmentation spectra interpretation, especially regarding correct PTM site localization. Our defined and carefully selected synthetic PTM reference peptides support PTM proteomics and help to overcome these challenges and are applicable for direct usage as reference material in mass-spectrometry based proteomics.

Available PTMs as peptide pools:
Phospho(serine, threonine, tyrosine), Arg(Me, Me2a, Me2s), Lys(Ac, Me, Me2, Me3, Cro, Suc, Mal, Biotin, Glu, For, Prop, But, Hib, GG), Glyco-Thr(alphaDGalNAc), Glyco-Ser(alphaDGalNAc). The peptides are part of a collection of 30 x 100 peptides that comprises wild-type peptides as well as 24 different post-translational modifications (PTMs)

Benefits of SpikeMix™ Non-quantified PTM Reference Kits
- Large range of PTM reference peptides
- Selected for high recovery in LC-MS
- 100 peptides in one pool

References for SpikeMix™ PTM-Kit 29 - Arg(Me)

References:
Read References with Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™

Testimonials for SpikeTides™
"My unit at the Banting and Best Department of Medical Research, University of Toronto Centre for Cellular and Biomolecular Research (CCBR) works on the systematic identification and quantification of proteins and protein complexes on a global level. In addition we have started to perform targeted proteomics to study protein regulation within pathways, e.g. signaling pathways during stem cell fate decision. For this we used JPT's various SpikeTides™ peptide products to build LC-MRM assays in a fast and cost efficient manner. More recently we also found new ways of using low cost SpikeTides™_TQL as quantitative peptide standards in order to quantify protein targets more accurately. We are currently in the process of validating this technology for the accurate quantification of various predicted microRNA targets."
Prof. Andrew Emili, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto

"My group studies the proteomic composition of distinct chromatin domains, the mechanisms that operate to maintain the composition of histone modifications and the associated proteins. For precise and accurate identification and quantification of histone peptides that carry multiple post-translational modifications directly from biological samples JPT's SpikeTides™ _TQL peptide standards proved to be of excellent value for our research in various projects."
Prof. Dr. Axel Imhof, Adolf-Butenandt Institute, University of Munich, Germany

Documentation for SpikeMix™ PTM-Kit 29 - Arg(Me)


Properties of SpikeMix™ PTM-Kit 29 - Arg(Me)

Application: Proteomics
Category: PTM Peptide Reference Standards
Condition / Topic: Control, PRM Assay, PTM Proteomics, SRM Assay
Layout: Freeze-dried in plastic vial
Modification: Methylation
Organism: Other/None
Protein Name: Selected proteins
Purity: crude
Quantification: No

Further Information to SpikeMix™ PTM-Kit 29 - Arg(Me)

Information Values
Sequence: See accompanying Excel file
WT Sequence:
AAHGRGALAEAAR;AAYQAAIRGVGSAQSR;AGRGFSLEELR;AMLPGSPLFTRAPPPK;ANYNFRGMYNQR;APPIDRSILPTAPR;ARGGCPGGEATLSQPPPR;CGLVIGRGGENVK;CVEGQLLRGLSK;DCGTLPNRFVITPR;DFAFYNPRLQGK;DRGVGAEPLLPWNR;DSRGGGGNFGPGPGSNFR;FGDSRGGGGNFGPGPGSNFR;FGSRGGPGGGFR;FRVTQPNTFGLFLYK;FSHSVQNSERGK;GAPGGRGAQLSVPSPAR;GAPLSSAPILGGWFGRGCTK;GARLPGPVSSAR;GFGRGGAESHTFK;GGFQNRGGGSGGGGNYR;GGGDRGGFGPGK;GGGYGGDRGGYGGDR;GGNFGGRGGYGGGGGGSR;GGPGGELPRGPQAGLGPR;GGPMRGGPGPGPGPYHR;GGSAGGRGFFR;GGSGGRGVSVSSAR;GIGRGIALQLCK;GIGRGTVQALHATGAR;GLATFPSRGAK;GMDTRGPVPGPR;GNDTFGPRAQGWAR;GNLEGPWGGGRGLR;GPPRGGPGGELPR;GRGAANDSTQFTVAGR;GRGLPGPAESLR;GRGTGEAEEEYVGPR;GRGTSQLAEGATAK;GRGVGSPEPGPTAPYLGR;GSGLGARGSSYGVTSTESYK;GSYLGGYSAGRGIYSR;GVPRGVHLGSQGLQR;HGGSGGRGVSVSSAR;HTLLRAPAPELLPANVAGR;IPSTICFGQGAARGFK;IQNGAQGIRFIYTR;IRMTHNLLLNYGLYR;LFHLPEYTWRLPCGGVR;LLALSGPGGGRGR;LVLPSQAQARLPSGEVVK;MEGSDFESSGGRGLK;MGAGMGFGLERMAAPIDR;MSEGRGLPPPPR;MSGRGVGSVSQFK;MTEDALRLNLLK;MTGSSGGDRGGFK;PLPVALQTRLAK;PRTPTAPAVNLAGAR;RGFGFGGFAISAGK;RGGSGSHNWGTVK;RGMAPASTDDLFAR;RGPPFPPPPPGAMFGASR;RGSFSSENYWR;RGTVGAGCGFNQR;RGVGAGAIAK;RGWGAAAVAAGLR;RVYYLSLEFYMGR;SFDEVEGVFGRGGGR;SGGGGYSQNRWGNNNR;SGGGYGGDRGGGYGGDR;SGGGYGGDRSSGGGYSGDR;SGGRGVSVSSAR;SGGYGGSRDYYSSR;SGQGAFGNMCRGGR;SGRGGNFGFGDSR;SGSNWGRGSNMNSGPPR;SMRGGYIGSTYFER;SQTGWPRGVTQFGNK;SRFYSGFNSR;SRGAIISQYYNR;SRGFGFITFTNPEHASVAMR;SRGGGGNFGPGPGSNFR;SRGLPCTELFVAPVGVASK;STNVTVAAARGIK;SYRGGGGGGGGWR;SYRSFSNLWK;TGSGYSRSSQGQPWR;TLHLSHLNRFPAR;TNAAAMNMGRPFQK;TPSSFRQPFTPTSR;TQAEGQGQGRGLGLVSAVR;VPLLGSRLPYQPPALCSLR;VTVARGSALEMEFK;YCLTAPNYRLK;YGRGQLNYPLPDFSK;YSRGAGLGFSTAPNK;YSSNLGNFNYEQRGAFR;YSSSSIPEPFGSRGSPR
Specifications: Proteotypic peptides

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