SPT-KAL-POOL-L-10pm
Two pools of representative stable isotope labeled proteotypic peptides from human kinase activation loops for direct usage in mass-spectrometry based proteomics (MRM). Set of 2 peptide pools: one contains 178 non-phosphorylated, the other 288 phosphorylated peptides.
Are you interested in your custom peptide pools for your proteomics assays? Choose your sequence, amount and label. We will assist you along the way. Custom SpikeMix Reference Pools.
JPT's SpikeMix™ Kinase Activation Loops (Human) - heavy
JPT produced 466 representative stable isotope labeled proteotypic peptides (178 non-phosphorylated, 288 phosphorylated) for human kinase activation loops for direct usage in mass-spectometry based proteomics. The peptides are available as set of individual peptides are as peptide mixes (pools) with stable isotope labeled (using heavy Arg U-13C6, U-15N4 or Lys U-13C6, U-15N2) peptides. We suggest to setup experimental conditions with the SpikeTides™ Set before using the SpikeMix™ peptide pools, due to complex detection of phosphopeptides.
Benefits of JPT's SpikeMix™ Kinase Activation Loops (Human) - heavy
- Quantification of multiple proteins from a single sample
- First economic heavy peptide pool available
- Multiplexed analysis of disease status and therapeutic success
- Presence of each peptide confirmed (by LC-MS or Maldi)
- Custom absolutely quantified peptides available upon request
References:
Read References with Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™
Application Notes
Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens
Soderblom et al., Application Note (2015) Full text
Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides
Bindel et al., Application Note (2015) Full text
Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides
Colangelo, Application Note (2014) Full text
Testimonials
"As Director of the Protein Profiling at Yale Keck Biotechnology Resource Facility, I coordinated a collaboration between the Association of Biomolecular Resource Facilities (ABRF) standard proteomic research group (sPRG) and JPT Peptide Technologies to develop the ABRF cross-species SpikeMix™ peptide pool. The joint development turned out to be an extraordinary effective endeavor and the resulting product was qualified in more than 52 proteomics labs around the globe. I was impressed by JPT's scientific and technological capabilities as well as their enthusiasm to drive the project forward."
Christopher M. Colangelo, Director of Protein Profiling at Keck Biotechnology Resource Facility, Yale University, USA
"Our research at Stanford University seeks to discover unconventional translation products, and proteins made by unsequenced organisms; to characterize a wide array of protein post-translational modifications; and to perform peptide antigen binding assays. On all of these fronts, we are in need of high quality peptide standards for assay development and protein quantification. The successful collaboration with the team at JPT Peptide Technologies and their wide range of products has been a critical factor for advancing my group's research."
Joshua E. Elias, Ph.D., Assistant Professor, Chemical & Systems Biology, Stanford University School of Medicine, Clark Center, Stanford, CA
Properties | Values |
---|---|
Application: | Cancer biomarker research, Enzymology, Proteomics |
Category: | SpikeMix™ for Targeted Proteomics |
Condition / Topic: | Breast cancer, Burkitt's lymphoma, Cancer, Gastric cancer, Malignant genital cancers, Melanoma, Prostate cancer |
Layout: | Freeze-dried in 2 plastic vials |
Organism: | Human |
Protein Name: | Kinases |
Purity: | crude |
Quantification: | Yes |
Information | Values |
---|---|
Sequence: | See Layout and Sequences under Product Documentation |
Specifications: | 466 representative stable isotope labeled proteotypic peptides (178 non-phosphorylated, 288 phosphorylated) for human kinase activation loops |
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