SpikeMix™ ABRF (cross-species standard)


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About SpikeMix™ ABRF (cross-species standard)

Pool of 1000 stable isotope labeled proteotypic peptides (SIL) from human, mouse and rat proteins to be used as standards across the three species. Peptide mixtures for protein quantification by mass spectrometry based proteomics (e.g. MRM).

SpikeMix™ ABRF (cross-species standard) - Specifications

Amount: 100 pmol/peptide
Purity: crude
Delivery Format: Freeze-dried in plastic vial
Application(s): Proteomics
Condition(s)/Topic(s): Control
Standard Delivery Time: 2-5 days

Are you interested in your custom peptide pools for your proteomics assays? Choose your sequence, amount and label. We will assist you along the way. Custom SpikeMix Reference Pools.

JPT's SpikeMix™ ABRF (cross-species standard)
JPT and the Proteomics Standards Research Group of ABRF developed a pool of 1000 heavy labeled proteotypic peptides for conserved proteins across these three species: rat, mouse and human. SpikeMixes™ enable the standardization of mass spectrometry based proteomics assays. The SpikeMix™ ABRF (cross-species standard) peptide pool was designed for quantitative mass spectrometric assay development and relative protein quantification. The mixed peptides are stable isotope labeled (heavy Arg U-13C6;U-15N4 or Lys U-13C6;U-15N2).

Benefits of JPT's SpikeMix™ ABRF (cross-species standard)
- Quantification of multiple proteins in a single sample
- Economic heavy labeled peptide pool of 1000 peptides
- Standardization of mass spectrometry based assays


References for SpikeMix™ ABRF (cross-species standard)

Read References with Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™

Application Notes
Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens
Soderblom et al., Application Note (2015) Full text
Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides
Bindel et al., Application Note (2015) Full text
Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides
Colangelo, Application Note (2014) Full text

"As Director of the Protein Profiling at Yale Keck Biotechnology Resource Facility, I coordinated a collaboration between the Association of Biomolecular Resource Facilities (ABRF) standard proteomic research group (sPRG) and JPT Peptide Technologies to develop the ABRF cross-species SpikeMix™ peptide pool. The joint development turned out to be an extraordinary effective endeavor and the resulting product was qualified in more than 52 proteomics labs around the globe. I was impressed by JPT's scientific and technological capabilities as well as their enthusiasm to drive the project forward."
Christopher M. Colangelo, Director of Protein Profiling at Keck Biotechnology Resource Facility, Yale University, USA

"Our research at Stanford University seeks to discover unconventional translation products, and proteins made by unsequenced organisms; to characterize a wide array of protein post-translational modifications; and to perform peptide antigen binding assays. On all of these fronts, we are in need of high quality peptide standards for assay development and protein quantification. The successful collaboration with the team at JPT Peptide Technologies and their wide range of products has been a critical factor for advancing my group's research."
Joshua E. Elias, Ph.D., Assistant Professor, Chemical & Systems Biology, Stanford University School of Medicine, Clark Center, Stanford, CA


Documentation for SpikeMix™ ABRF (cross-species standard)


Properties of SpikeMix™ ABRF (cross-species standard)

Properties Values
Application: Proteomics
Category: SpikeMix™ for Targeted Proteomics
Condition / Topic: Control
Layout: Freeze-dried in plastic vial
Organism: Human, Mouse
Protein Name: Selected proteins
Purity: crude
Quantification: Yes

Further Information to SpikeMix™ ABRF (cross-species standard)

Information Values
Sequence: See Layout and Sequences under Product Documentation
Specifications: Mix of 1000 heavy labeled proteotypic peptides for conserved proteins
TÜV Rheinland certified
Health - made in Germany
Quality Assurance

All production is ISO 9001:2015 certified.

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