PPK-1-10pmol
Collection of 24 non-naturally occurring phosphopeptides for standardization of phosphoproteomics workflows and retention times in HPLC-MS based phosphoproteomics experiments.
Are you interested in your custom peptide libraries for your proteomics assays? Choose your sequence, amount and label. We will assist you along the way. Custom SpikeTides Reference Peptides.
Proteomics Phospho Kit
Phosphoproteomics has matured into a strong technology for the simultaneous detection of phosphopeptides in complex samples. However, phosphoproteomics workflows consist of several steps that all need to be controlled carefully (Urban, J., Analytica Chimica Acta 2021, 338857). If phosphoproteomics is performed in a targeted fashion, HPLC retention time (RT) windows for the selected proteotypic phosphopeptides need to be established carefully, as RTs are not easily transferable between instruments.
Benefits of Proteomics Phospho Kit
- Non-naturally occurring peptides (allowing application in biological samples)
- Non-isotopically labeled peptides (facilitating inclusion into database searches)
- Broad LC gradient coverage
- Stable RTs across multiple injections
- Use for column performance evaluation
- Use for optimization of LC-MS gradients
References:
Read References with Reference Peptides for Targeted Proteomics - SpikeTide™ & SpikeMix™
Application Notes
Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens
Soderblom et al., Application Note (2015) Full text
Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides
Bindel et al., Application Note (2015) Full text
Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides
Colangelo, Application Note (2014) Full text
Fast and Accurate Determination of Cysteine Reduction and Alkylation Efficacy in Proteomics Workflows
Schnatbaum, Application Note (2016) Full text
| Properties | Values |
|---|---|
| Application: | Proteomics |
| Category: | Proteomics Standards |
| Condition / Topic: | Control, PTM Proteomics |
| Layout: | Freeze-dried in plastic vial |
| Modification: | Phosphorylation |
| Organism: | Other/None |
| Protein Name: | Other |
| Quantification: | No, Yes |
| Information | Values |
|---|---|
| Sequence: | See Product Documentation |
| Specifications: | 24 non-naturally occurring phosphopeptides |
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