product protein interaction screen on matrix prisma step 1

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Protein Interaction Screen on Peptide Matrix (PRISMA)

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Protein-protein interactions are a hallmark of signal transmission and key to understanding regulatory mechanisms. Therefore, we developed the Protein Interaction Screen on Peptide Matrix (PRISMA) together with our partners from Max-Delbruck-Center for Molecular Medicine (MDC) to systematically explore the interactome.

Peptides are synthesized on a cellulose membrane
Peptides of up to 25 amino acids can represent sequence diversity and contain post-translational modifications
The membrane is incubated with a biological sample for affinity enrichment of soluble proteins and protein complexes
Proteins interacting with the peptide matrix are then identified and quantified by mass spectrometry

Applications

Applications for Protein Interaction Screen on Peptide Matrix (PRISMA)

Identification of protein-protein interaction partners
Systematic exploration of the interactome
Influence of post-translational modifications decoded
Discovery of the influence of sequence diversity on protein interactions
Comparison of cell states
Identification of complexes
Mapping of pathways

Benefits

Benefits of Protein Interaction Screen on Peptide Matrix (PRISMA)

Hundreds of protein interactions can be detected and identified as potential interaction partners
Modulatory effects of numerous PTMs and mutations can be assessed

Quantification of proteins possible
Validated assay based on comparison with other affinity enrichment approaches, conventional immunoblotting analysis, and co-occurrence

References

Quality Assurance

All production is ISO 9001:2015 certified.