Universal T Cell Assay Standard TERS™ Kit

Performance control of T-cell assays using TCR-engineered reference samples (TERS™)

The TERS™ technology is based on a simple and efficient protocol to create T-Cell-Receptor-engineered PBMC samples with a defined number of antigen-specific T cells (TCR-engineered reference samples or "TERS" for short) by means of an antigen encoding TCR RNA. The TERS cells can be used in combination with:

  • MHC-Multimer staining
  • Intracellular cytokine detection
  • ELISPOT assays

The reference samples, produced with the Universal T Cell Assay Standard TERS™ Kit, address an important requirement for standardization controls. A lack of standard control reagents for immunological T-cell assays has been limiting the comparability of antigen-specific test results, whether these originate from the same laboratory at different times, from different platforms, or from different laboratories.

Producing TERS with the Universal T Cell Assay Standard TERS™ Kit

  • Transfection of PBMCs with the provided TCR mRNA using electroporation
  • Quality control by flow-cytometry
  • Defined number of TCR-positive T cells is spiked in untouched PBMCs
  • Final cell suspension holds the desired number of viable transfected CD8-positive T cells
  • Aliquots of this suspension are frozen for subsequent use
  • GFP RNA is included for optimizing electroporation conditions
  • A detailed protocol for all experimental steps is provided

Applications for Universal T Cell Assay Standard TERS™ Kits

  • Standardization & validation of T-cell assays (ELISPOT, ICS, MHC-Multimer staining)
  • Performance control of T-cell assays
  • Calibration of antigen-specific assays

Benefits of Universal T Cell Assay Standard TERS™ Kits

  • Comparability of antigen-specific test results across different time points, different platforms, or different laboratories - Signals are comparable to patient material
  • Quantitative information on assay performance
  • Easy production of TERS™
  • All required RNA reagents available in one kit

References for Universal T Cell Assay Standard TERS™ Kits

"Generation of TCR-Engineered T Cells and Their Use To Control the Performance of T Cell Assays”
Bidmon et al., J. Immunol. (2015)

More References with RNA Tools

 

 

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