ULTRA-Peptide Libraries for Sequence Diversity
As sequence diversity among viruses and in humans is gaining more attention in research and development efforts, we created a new algorithm to reflect sequence diversity within our peptide libraries, peptide pools and peptide microarrays.
Sequence Diversity in Viruses
- Sequence diversity is a hallmark of many pathogenic viruses including HIV, HBV and Influenza
- Represents a great challenge for vaccine development, biomarker discovery and immune monitoring
- Greatest sequence diversity has been found in viral envelope proteins, due to their important role in eliciting host immune recognition
- Sequence diversity has to be taken into account in the design of antigen-based products, intended for immune monitoring and vaccination
Sequence Diversity in Tumors
- The 1000 Genomes and other projects provide information on the genetic variability between individuals
- 10000 to 12000 sites with coding mutations have been identified per individual translating into a sequence alteration in one of every two genes
- Somatic mutations cause sequence heterogeneity within individuals which are of special significance in the pathological mechanisms of cancer
- 767 germline and 29881 somatic mutations have been identified for TP53, one of the most important tumor driver genes
Applications for JPT’s ULTRA-Peptide Libraries
- Immune monitoring of T- and B-cell response
- T-cell stimulation in immunotherapy
- Biomarker discovery
- Development of vaccines
Read Application Notes:
Application Note on the Generation of ULTRA Libraries
by Pawlowski et al. (2015)
Application Note on the Use of HIV Ultra-Peptide Pools to Generate Multi-HIV Antigen T-Cells as a Component for a Cure Strategy
by Lam et al. (2015)
Benefits of ULTRA-Peptide Libraries
- Allows high coverage even of complex sequence spaces
- Proprietary bioinformatic algorithm to design your optimal peptide library
- Fast chemical assembly of complex peptide libraries, pools, and microarrays
- Maximum diversity at minimum number of peptides
References on ULTRA-Peptide Libraries
"Antibody Responses After Analytic Treatment Interruption in Human Immunodeficiency Virus-1-Infected Individuals on Early Initiated Antiretroviral Therapy"
Stephenson et al., Open Forum Infectious Diseases (2016) - PMID: n.a.
"A Global Reference for Human Genetic Variation"
The 1000 Genomes Project Consortium, Nature (2015) - PMID: 26432245
"Impact of Mutant p53 Functional Properties on TP53 Mutation Patterns and Tumor Phenotype: Lessons From Recent Developments in the IARC TP53 Database"
Petitjean et al., Hum. Mut. (2007) - PMID: 17311302
"Broadly-specific Cytotoxic T Cells Targeting Multiple HIV Antigens Are Expanded From HIV+ Patients: Implications for Immunotherapy"
Lam. et al., Mol. Ther. (2015) - PMID: 25366030
"Quantification of the Epitope Diversity of HIV-1-Specific Binding Antibodies by Peptide Microarrays for Global HIV-1 Vaccine Development"
Stephenson et al., J. Immunol. Methods (2015) - PMID: 25445329
"Comprehensive Characterization of Antibodies Directed towards Epigenetic Histone Modifications"
Masch. et al., Application Note (2015)
Read More References
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All production is performed according to ISO 9001:2015 standards