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Stable Isotope-Labeled Peptides & Pools

JPT developed SpikeMix™ - an inexpensive source of customized and ready-to-use, stable isotope labeled peptide standard pools for use in mass spectrometry based proteomics. Additionally we compiled sets of absolutely quantified and stable isotope labeled peptides as low cost source of quantified peptides. They are used as peptide standards in mass spectrometry proteomics (i.e. MRM/ SRM assays).

In addition, JPT provides the highly flexible production of customized SpikeMix™ peptide standard pools. Our stable isotope-labeled peptides contain a heavy arginine (U-13C6; U-15N4) or lysine (U-13C6; U-15N2).

Non-Quantified Peptides

SPT-ABRF-POOL-L-100pmSpikeMix™ ABRF (cross-species standard) - 100pmol Pool of 1000 stable isotope-labeled proteotypic peptides from conserved human, mouse and rat proteins to be used as standard across the three speciesInfo
SPT-ABRF-POOL-L-10pmSpikeMix™ ABRF (cross-species standard) - 10pmol Pool of 1000 stable isotope-labeled proteotypic peptides from conserved human, mouse and rat proteins to be used as standard across the three speciesInfo
SPT-ABRF-POOL-L-1pmSpikeMix™ ABRF (cross-species standard) - 1pmol Pool of 1000 stable isotope-labeled proteotypic peptides from conserved human, mouse and rat proteins to be used as standard across the three speciesInfo
SPT-CEF-E-POOL-LSpikeMix™ CEF (ext) - heavy Pool of 32 stable isotope labeled peptides selected from defined HLA class I-restricted T-cell epitopes derived from CMV, EBV and Influenza. Peptide mixture for quantification by mass-spectrometry based proteomics Info
SPT-CYT-POOL-L-bovSpikeMix™ Cytokines (Bovine) - heavy Pool of 224 stable isotope-labeled proteotypic peptides for bovine cytokines (e.g. interleukines, intereferons, lymphokines). Stable isotope labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM, SRM)Info
SPT-CYT-POOL-L-cynSpikeMix™ Cytokines (Cynomolgus monkey) - heavy Pool of 141 stable isotope-labeled proteotypic peptides for cytokines of Cynomolgus monkey (Macaca fascicularis). Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-CYT-POOL-L-canSpikeMix™ Cytokines (Dog) - heavy Pool of 144 stable isotope-labeled proteotypic peptides for dog (canis familiaris) cytokines. Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-CYT-POOL-L-guiSpikeMix™ Cytokines (Guinea Pig) - heavy Pool of 134 stable isotope-labeled proteotypic peptides for cytokines of Guinea Pig (Cavia porcellus). Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-CYT-POOL-L-humSpikeMix™ Cytokines (Human) - heavy Pool of 461 heavy isotope labeled proteotypic peptides for 361 human cytokines (e.g. interleukines, intereferons, lymphokines). Stable isotope labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM, SRM)Info
SPT-CYT-POOL-humSpikeMix™ Cytokines (Human) - light Pool of 461 representative proteotypic peptides for 361 human cytokines (e.g. interleukines, intereferons, lymphokines). Mixture of unlabeled peptides for rapid SRM and MRM assay setupInfo
SPT-CYT-POOL-L-mouSpikeMix™ Cytokines (Mouse) - heavy Pool of 383 heavy isotope labeled proteotypic peptides for murine cytokines (e.g. interleukines, intereferons, lymphokines). Stable isotope labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM, SRM)Info
SPT-CYT-POOL-L-rabSpikeMix™ Cytokines (Rabbit) - heavy Pool of 123 stable isotope-labeled proteotypic peptides for rabbit (Oryctolagus cuniculus) cytokines. Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-CYT-POOL-L-ratSpikeMix™ Cytokines (Rat) - heavy Pool of 226 stable isotope-labeled proteotypic peptides for rat (rattus norvegicus) cytokines. Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-CYT-POOL-L-rheSpikeMix™ Cytokines (Rhesus macaque) - heavy Pool of 156 stable isotope-labeled proteotypic peptides for cytokines of Rhesus macaque (Macaca mulatta). Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-CYT-POOL-L-oviSpikeMix™ Cytokines (Sheep) - heavy Pool of 182 stable isotope-labeled proteotypic peptides for sheep (Ovis aries) cytokines. Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-CYT-POOL-L-calSpikeMix™ Cytokines (White-tufted-ear marmoset) - heavy Pool of 119 stable isotope-labeled proteotypic peptides for cytokines of White-tufted-ear marmoset (Callithrix jacchus). Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-CYT-POOL-L-xenSpikeMix™ Cytokines (Xenopus) - heavy Pool of 54 stable isotope-labeled proteotypic peptides for cytokines of Xenopus (Xenopus laevis). Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-CYT-POOL-L-zebSpikeMix™ Cytokines (Zebrafish) - heavy Pool of 109 stable isotope-labeled proteotypic peptides for Zebrafish (Danio rerio) cytokines. Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-KAL-POOL-L-100pmSpikeMix™ Kinase Activation Loops (Human) - heavy - 100pm Two pools of representative stable isotope labeled proteotypic peptides for human kinase activation loops Info
SPT-KAL-POOL-L-10pmSpikeMix™ Kinase Activation Loops (Human) - heavy -10pm Two pools of representative stable isotope labeled proteotypic peptides for human kinase activation loops Info
SPT-PH-POOL-L-hum -10pmSpikeMix™ Peptide Hormones (Human) - heavy - 10 pmol Pool of more than 500 stable isotope-labeled peptides for human peptide hormones. Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-PH-POOL-L-hum-100pmSpikeMix™ Peptide Hormones (Human) - heavy - 100 pmol Pool of more than 500 stable isotope-labeled peptides for human peptide hormones. Heavy labeled peptide mixture for relative quantification by mass-spectrometry based proteomics (MRM assays, SRM)Info
SPT-ORG-POOL-LSpikeMix™ Plant Organelle Marker (Arabidopsis thaliana) - heavy Pool of 67 stable isotope-labeled organelle marker peptides from Arabidopsis thaliana organelles. It covers 14 major subcellular locations: Cytosol, Cytosol (cytosolic ribosomes), ER, Extracellular, Golgi, Mitochondria, Nucleus, Peroxisome, Plastid (LHC),Info
SPT-TAA-POOL-LSpikeMix™ TAA - heavy Pool of 252 representative heavy isotope labeled proteotypic peptides for tumor associated antigens derived from 61 human cancer related proteins (e.g. MAGE, WT-1, ERBB2, p53, Myc). Peptide mixture for quantification by mass-spectrometry based proteomics Info
SPT-TAA-POOLSpikeMix™ TAA - light Pool of 252 representative proteotypic peptides for tumor associated antigens derived from 61 human cancer related proteins (e.g. MAGE, WT-1, ERBB2, p53, Myc). Peptide mixture for quantification by mass-spectrometry based proteomics (MRM)Info
SPT-Wnt-POOL-L-100pmSpikeMix™ Wnt Signaling Pathway (Human) - heavy - 100pmol Pool of 343 stable isotope labeled proteotypic peptides for 65 proteins of the human Wnt pathway. Peptide mixture for quantification by mass-spectrometry based proteomics Info
SPT-Wnt-POOL-L-10pmSpikeMix™ Wnt Signaling Pathway (Human) - heavy - 10pmol Pool of 343 stable isotope labeled proteotypic peptides for 65 proteins of the human Wnt pathway. Peptide mixture for quantification by mass-spectrometry based proteomics Info
SPT-CYT-L-humSpikeTides™ Set Cytokines (Human) - heavy Collection of 461 heavy labeled proteotypic peptides for 219 human cytokines (e.g. interleukines, intereferons, lymphokines). Stable isotope labeled for usage in mass-spectrometry based proteomics (MRM, SRM)Info
SPT-CYT-humSpikeTides™ Set Cytokines (Human) - light Collection of 461 representative proteotypic peptides for 219 human cytokines (e.g. interleukines, intereferons, lymphokines). Unlabeled peptides for rapid SRM assay setup and other mass-spectrometry based proteomics assays (MRM)Info
SPT-KAL-LSpikeTides™ Set Kinase Activation Loops (Human) - heavy Collection of 466 representative stable isotope labeled proteotypic peptides (178 non-phosphorylated, 288 phosphorylated) for human kinase activation loops Info
SPT-TAA-LSpikeTides™ Set TAA - heavy Collection of 252 representative isotopically labeled proteotypic peptides derived from 61 prioritized tumor associated antigensInfo
SPT-TAASpikeTides™ Set TAA - light Collection of 252 representative proteotypic peptides derived from 61 prioritized tumor associated antigensInfo

Custom SpikeMix™ Proteotypic Peptide Pools

Stable isotope labeled proteotypic peptides mixed and aliquoted according to your specifications.
Alkylation and post-translational modifications (PTMs such as phosphorylation, glycosylation, methylation and others) upon request.

Pricing:
$9.95 / peptide (250 - 499 peptides)
$9.50 / peptide (500 - 999 peptides)
$8.50 / peptide (from  1000 peptides)

For further information and quote request please visit our custom SpikeTides™ Proteotypic Peptides page.

Applications for Proteotypic Peptide Sets & Pools

  • Cancer biomarker (tumor associated antigens) discovery and validation
  • Clinical monitoring of therapeutic intervention and disease status
  • Mass spectrometry based assays (SISCAPA, MRM, etc.) 
  • Identification & optimization of assay conditions for proteotypic peptides
  • Quantification of immunomodulating agents
  • Standardization of mass spectrometry based proteomics assays

Application Notes:
"Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides"
by F. Bindel, G. Mastrobuoni, A. Eisenberger & S. Kempa from Integrative Metabolomics and Proteomics Laboratory, Max Delbrueck Center, Berlin, Germany
"Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens"
by E. Soderblom from Proteomics and Metabolomics Core Facility at Duke University Medical Center
"Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides"
by C.M. Colangelo, Director of Protein Profiling at Yale University and Chair of the Proteomics Standards Research Group (ABRF).

Benefits of Proteotypic Peptide Sets & Pools

  • Quantification of multiple antigens or cytokines from a single sample
  • Economic heavy peptide sets and pools
  • Multiplexed analysis of disease status and therapeutic success
  • For tailored proteotypic peptides, please refer to our customized proteotypic peptides SpikeTides™

The use of this product may be subject of U.S. patent No. 7,501,286, the European patent 1,472,539 and corresponding patent applications owned by the President and Fellows of Harvard College, USA. The purchase of this product does not convey a license under any method claims in the foregoing patents or patent applications.

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Quality Assurance

All production is performed according to ISO 9001:2015 standards

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