Custom SpikeMix™ - Reference Peptide Pools

SpikeMix™ Peptide Pools are inexpensive and reliable custom stable-isotope-labeled peptide pools for relative protein quantification by mass spectrometry-based assays in targeted proteomics.

  • For targeted proteomics (protein detection and protein quantification by mass spectrometry)
  • Proteotypic stable-isotope-labeled peptides (SIL peptide pools)
  • Inexpensive & fast
  • Optional modifications such as Alkylation, PTMs (phosphorylation, glycosylation, methylation…)
  • All SIL peptides contain a heavy arginine or heavy lysine

Also available:



Applications

Applications for Custom SpikeMix™ - Reference Peptide Pools

  • Development of mass spectrometry-based proteomics assays
  • Relative quantification of proteins
  • Development of kits to quantify entire proteomic pathways
  • Clinical monitoring of therapeutic intervention and disease status
  • Mass spectrometry based assays (SISCAPA, MRN, etc.)

Application Note:

SpikeTidesTM - Proteotypic Peptides for Large-Scale MS-Based proteomics
Schnatbaum et al. (2011) Full Text

Benefits

Benefits of Custom SpikeMix™ - Reference Peptide Pools

  • Unmatched turnaround times and prices
  • Aliquotation service available
  • Quantification of multiple proteins from a single sample
  • Multiplexed analysis of disease status and therapeutic success

Testimonials

Testimonials for Custom SpikeMix™ - Reference Peptide Pools

"At Cambridge University we have significantly benefited from a suite of SpikeTides™ from JPT which have been developed for use as organelle markers in Arabidopsis. Using these we have developed faster and more accurate methods, using SRM and PRM, for estimating organelle enrichment along linear density gradients. Results have been very instrumental in optimizing downstream methodologies, a process which has delivered improved resolution in ensuing datasets for lower costs than our original methods."
Harriet Parsons, PhD (Department of Biochemistry, University of Cambridge, UK)

"At MSKCC, we have been using JPT for isotopically labeled peptide libraries for developing pathway- and genome-scale atlases for quantitation of cellular signaling. This has been invaluable for the Quantitative Cell Proteomics Atlas (QCPA), a research tool for precise and sensitivity quantitation of cellular signaling, as well as clinical diagnostic assays for medical use."
Alex Kentsis, MD, PhD (Memorial Sloan Kettering Institue, New York, USA)


References

References for Custom SpikeMix™ - Reference Peptide Pools

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