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Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™

SpikeTides™ and SpikeMix™ are the inexpensive and reliable sources of customized stable isotope labeled peptides (heavy peptides) or light reference peptides and reference peptide pools for targeted proteomics (relative protein quantification by mass spectrometry-based assays).

  • Individual reference peptides (SpikeTides™) or pooled peptides (SpikeMix™)
  • For targeted proteomics (protein detection and protein quantification by mass spectrometry)
  • Proteotypic or other selected peptides
  • Inexpensive & fast delivery (10 000 peptides/ week)
  • Light or stable isotope-labeled peptides (SIL peptides)
  • Optional modifications such as PTMs & cysteine carbamidomethylation
  • SIL peptides contain a heavy arginine (U-13C6; U-15N4) or heavy lysine (U-13C6; U-15N2)
  • For absolute quantified reference peptides please check Absolute Quantified Peptides - SpikeTides™ TQL & SpikeTides™ TQL PLUS!

Also available: Catalog SpikeTides™ & SpikeMix™ Kits comprising selected tumor associated antigens (TAAs), cytokines from different species or PTM Reference Peptides & Kits and Standardization Kits (see below). 

Select Your Reference Peptides for Targeted Proteomics

SpikeMix™ – stable isotope-labeled proteotypic peptide pools

SpikeMix™ – stable isotope-labeled proteotypic peptide pools

Heavy isotope labeled proteotypic peptides mixed and aliquoted according to your specifications. Alkylation and post-translational modifications (PTMs such as phosphorylation, glycosylation, methylation and others) upon request.

Pricing:
$9.95 / peptide (250 - 499 pooled peptides)
$9.50 / peptide (500 - 999 pooled peptides)
$8.50 / peptide (from 1000 pooled peptides) 

Please also check our catalog SpikeMixes™for  tumor associated antigens, cytokine and standardization proteins on the Proteotypic Heavy Peptide Pools & Sets (SpikeMixes™ & SpikeTides™ Sets) page.

SpikeTides™ - light proteotypic peptides

SpikeTides™ Proteotypic Peptides

SpikeTides™ are customized, inexpensive, proteotypic peptides that terminate as C-terminal Arg/Lys.
Amounts: 30nmol/peptide
Purity: Unpurified
Application: Optimization and validation of multiplexed SRM assays 
Price: from 9 US$ / 8 € (depending on number of peptides ordered)
Validated pooling service available upon request!

Spike Tides

SpikeTides™_L - isotopically labeled peptides

SpikeTides™_L Proteotypic Peptides

SpikeTides™_L are isotopically labeled, proteotypic peptides that terminate with C-terminal heavy Arg/Lys.
Amounts: appr. 10-30nmol/peptide (exact amount unknown!)
Purity: Unpurified
Application: Development of SRM assays and relative quantification of proteins using a single product
Price: from 19 US$ / 17 € (depending on number of peptides ordered)
Validated pooling service available upon request!

Spike Tides L

The use of this product may be subject of U.S. patent No. 7,501,286, the European patent 1,472,539 and corresponding patent applications owned by the President and Fellows of Harvard College, USA. The purchase of this product does not convey a license under any method claims in the foregoing patents or patent applications.

Maxi SpikeTides™ - options for large peptide amounts

Of course, all SpikeTides™ options can be adjusted to meet your requirements. Maxi SpikeTides™ are synthesized by standard resin-based custom peptide synthesis and very flexible. Choosing Maxi SpikeTides™ enables:

  • longer peptides
  • higher amounts per peptide
  • guaranteed purity for each peptide
  • no minimum peptide number
  • classical AAA quantification of peptides available
  • validated pooling service available

    Please inquire for your Maxi SpikeTides™ via email!

    Applications for Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™

    • Development, optimization and validation of mass spectometry based proteomics assays
    • Relative quantification of proteins
    • Selection of proteotypic peptides with high mass spectrometry signal response
    • Find predominant peptide fragments specific for your proteotypic peptide to be used in MRM transition
    • Develop kits to quantify entire proteomic pathways
    • Clinical monitoring of therapeutic intervention and disease status
    • Mass spectrometry based assays (SISCAPA, MRN, etc.)

      Application Note:
      SpikeTides - Proteotypic Peptides for Large-Scale MS-Based proteomics
      by Karsten Schnatbaum

    Benefits of Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™

    • Unmatched turnaround times (10 000 peptides/week!) and prices
    • Delivery in ready-to-use microtiter plates or vials
    • Aliquotation and mixing service available
    • Quantification of multiple  proteins from a single sample
    • Economic SIL peptide libraries and pools
    • Multiplexed analysis of disease status and therapeutic success

    Testimonials for Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™

    "At Cambridge University we have significantly benefited from a suite of SpikeTides™ from JPT which have been developed for use as organelle markers in Arabidopsis. Using these we have developed faster and more accurate methods, using SRM and PRM, for estimating organelle enrichment along linear density gradients. Results have been very instrumental in optimizing downstream methodologies, a process which has delivered improved resolution in ensuing datasets for lower costs than our original methods."
    Harriet Parsons, PhD (Department of Biochemistry, University of Cambridge, UK)

    "At MSKCC, we have been using JPT for isotopically labeled peptide libraries for developing pathway- and genome-scale atlases for quantitation of cellular signaling. This has been invaluable for the Quantitative Cell Proteomics Atlas (QCPA), a research tool for precise and sensitivity quantitation of cellular signaling, as well as clinical diagnostic assays for medical use."

    Alex Kentsis, MD, PhD (Memorial Sloan Kettering Institue, New York, USA)

    More testimonials

    Spike Tides-Synthetic Proteotypic Peptides

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    Quality Assurance

    All production is performed according to ISO 9001:2015 standards

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