Internally Quenched Peptides / FRET Peptides

JPT’s internally quenched peptides or FRET peptides are guaranteed to be free of fluorescent impurities. The most frequently used dye/quencher combinations and common attachment options are shown below.

Fluorophore Quencher Excitation /
Emission Wavelength
Abz
2-Aminobenzoyl
Dnp
2,4-dinitrophenyl
320 nm
420 nm
Abz
2-Aminobenzoyl
NitroTyr
3-nitro-tyrosine
320 nm
420 nm
Mca
7-Methoxycoumarin-4-yl)acetyl
Dnp
2,4-dinitrophenyl
325 nm
392 nm
Trp
Tryptophan
Dnp
2,4-dinitrophenyl
280 nm
360 nm
EDANS
5-[(2-Aminoethyl) amino] naphthalene-1-sulfonic acid
Dabcyl
4-([4'-dimethylamino)phenyl]azo)benzoyl
340 nm
490 nm
FAM
Carboxyfluorescein
Dabcyl
4-([4'-dimethylamino)phenyl]azo)benzoyl
492 nm
517 nm
Lucifer Yellow Dabsyl
4-(Dimethylamino)azobenzene-4'-sulfonyl
430 nm
520 nm

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