ProteaseSpots™
JPT Peptide Technologies offers a new and efficient way to study protease activities and substrate specificities. ProteaseSpots™ are custom synthesized protease substrate peptides that are immobilized on cellulose discs. The discs are delivered ready-to use in microtiter plates. During incubation with protease samples fluorescent detection can be performed after several time points.
Figure: The protease assay is based on peptides bound to small cellulose discs in 96-well microtiter plates.
These peptides bear a fluorescent moiety at the N-terminus. After incubation with the active protease, aliquots are transferred to an additional plate for detection of the N-terminally labeled peptide fragment (excitation: 325 nm, emission: 420 nm).
Applications for ProteaseSpots™
- Detection of protein cleavage sites utilizing peptide scans
- Identification of novel substrates with de novo libraries
- Measurement of protease activities with known substrates
- Characterization of cleavage sites through substitutional analyses
Benefits of the ProteaseSpots™ System
- Screen large numbers of substrates economically
- You specify the individual substrate sequences
- Obtain semi-kinetic data
- Easy handling in 96-well plates
Related products and services
Protease Substrate Sets
Internally quenched (Dabcyl/EDANS) pre-made protease substrate collections ready-to-screen in microtiter plates
Peptide Microarray Assay Service
Profiling service using high content PepStar peptide microarrays
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Protease Spots
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Quality Assurance
All production is performed according to ISO 9001:2015 standards