Peptide ELISA

Peptide ELISA (Enzyme-linked immunosorbent assay) enables analysis and screening on amino acid sequence level, e.g. mapping of epitopes or definition of protein interaction sites, thus providing much more information than conventional ELISA.
We offer custom peptide ELISA plates with optional incubation and assay service and an off-the-shelf Histone Peptide ELISA for screening of PTM specific antibodies or enzymes.

Tailored Peptide ELISA

Send us your peptide sequences and receive your tailored peptide ELISA. We offer various specifications to meet every requirement.
Please choose from the following options:

  • Peptide ELISA specification:
    • Discovery Grade: Unpurified peptides but truncated sequences are removed during immobilization
    • Validation Grade: Custom Peptides with full HPLC-MS analysis and guaranteed purity
  • Peptide ELISA plate format:
    • 96 well plate
    • 12 x 8 well strips

Applications for Peptide ELISA

  • Antibody epitope mapping
  • Immune profiling
  • Determination of antibody titers
  • Analysis of protein-protein interactions
  • Analysis of enzymatic reactions
  • Validation of microarray results

Application Note:
"A Modular Approach for Epitope Discovery and High-Resolution Profiling of Humoral Immune Responses"
by N. Pawlowski et al

Benefits of Peptide ELISA

  • High batch-to-batch reproducibility
  • Economic production of tailored ELISA plates
  • Easy-to-use and compatible with standard ELISA protocols and equipment
  • Generation of quantitative results
  • High sensitivity

References for Peptide ELISA

"Chimeric L2-Based Virus-Like Particle (VLP) Vaccines Targeting Cutaneous Human Papillomaviruses (HPV)"
Huber et al., PLoS One (2017) - PMID: 28056100
"Evaluating the Efficacy of Aluminium Phosphate Formulated L2 Based HPV Vaccine"
Lakshmikanth et al., Asian Journal of Pharmaceutical and Clinical Research (2015) - PMID: n.a
"Identification of Novel Antiacetylated Vimentin Antibodies in Patients with Early Inflammatory Arthritis"
Juarez et al., Ann Rheum Dis (2015) - PMID: n.a
"Development of β-Lactoglobulin-Specific Chimeric Human IgEκ Monoclonal Antibodies for In Vitro Safety Assessment of Whey Hydrolysates"
Knipping et al., PloS One (2014) - PMID: 25153680
"Antibodies Reacting with JCPyV_VP2 _167-15mer as a Novel Serological Marker for JC Polyomavirus Infection"

Lagatie et al., Virology Journal (2014) - PMID: 25273879
"Lack of Confirmation of Anti-inward Rectifying Potassium Channel 4.1 Antibodies as Reliable Markers of Multiple Sclerosis"
Nerrant et al., Mult Scler. (2014) - PMID: 24756568
"Peptide Microarrays Enable Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic Antibody IMAB362"
Schnatbaum et al., Biotechnol J. (2014) - PMID: 24497417
"Trans-activation, Post-Transcriptional Maturation, and Induction of Antibodies to HERV-K (HML-2) Envelope Transmembrane Protein in HIV-1 Infection"

Michaud et al., Retrovirology (2014) - PMID: 24472118

Read More References with Peptide ELISA

Related Products

PepStar™ Peptide Microarrays
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Histone Code Peptides, Microarrays & ELISA
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Epitope Discovery Service
Take advantage of our comprehensive Full Service (incl. incubation, analyses and detailed report)

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