PepSup™ Cell Activation Kit
PepSup™ is a highly standardized, peptide-based cell activation (cell stimulation) assay designed to produce short-term culture supernatants for analysis of cytokines or other mediators of interest. We create your PepSup™ Cell Activation Kit with your choice of PepMix™ Peptide Pools, Antigen Peptides or custom peptides using a specialized culture medium. A PepSup™ kit contains sufficient reagents for 6 cell stimulations with the pertinent positive and negative controls.
- High degree of standardization and hence reproducibility of cellular assays
- Well-suited for longitudinal monitoring
- Applicable for whole blood or other cell suspensions
- Monitor antigen-specific, stimulation-induced mediator secretion
Benefits of PepSup™ Cell Activation Kit
- Get your PepSup™ from any PepMix™ Peptide Pool or Antigen Peptide
- Use whole blood or other cell suspensions
- Only a heat block and a simple bench-top centrifuge required
- Samples can be used immediately or frozen for later analysis
- High degree of standardization and reproducibility
- Well-suited for longitudinal monitoring
Applications for PepSup™ Cell Activation Kit
- Monitor antigen-specific, stimulation-induced mediator secretion
- Study infection, transplantation, or tumor-specific immunity
- Produce short-term culture supernatants for analysis of cytokines or other mediators of interest
Application Note
"PepSup™ - A Simple T-cell Assay Providing the Highest Level of Standardization & Reproducibility"
by P. Holenya, T. Teck & F. Kern
spotmix™ | spotmix™ plus | pepmix™ | pepsup™ | |
---|---|---|---|---|
Amount/Peptide | Varying (approx. 5 to 20 µg / peptide depending on synthesis yield) | 10 µg / peptide (each peptide quantified) | 15 nmol (appr. 25µg) per peptide (each peptide quantified) | 6x 2 µg /peptide (each peptide quantified) |
Delivery Format | Freeze-dried | Freeze-dried | Freeze-dried | In PepSup™ cell medium |
Peptide Purity | Purified by Dialysis | Purified by Dialysis | Purified by HPLC | Purified by HPLC |
Peptide QC | 5% peptides analyzed by LC-MS | 100% peptides analyzed by LC-MS | 100% peptides analyzed by LC-MS | 100% peptides analyzed by LC-MS |
Pool QC | none | none | Full QC for each peptide pool warranting presence of each peptide | Full QC for each peptide pool warranting presence of each peptide |
Applications | Antigen discovery | Neo-epitope based immune monitoring, Antigen discovery | Clinical immune monitoring, preclinical & clinical research | Clinical immune monitoring, preclinical & clinical research, immunotherapy |
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PepSup™ Cell Activation Kit
News Highlights
- 72 New Antigen Peptides & PepMixes™ available!
Peptide synthesis in Germany acc. to ISO 9001:2015 avoiding toxic contaminants and side products.
(Nov 2018) -
Superior Positive Control Pools for Better T-Cell Assays
(May 2018)
Quality Assurance
All production is performed according to ISO 9001:2015 standards