PepStar™ Peptide Microarrays

JPT's unique PepStar™ peptide microarrays are high-content or multiwell peptide arrays on glass slides. Peptide arrays (or peptide chips) are used for biomarker discovery, immunomonitoring, antibody epitope mapping, multiplexed epitope mapping or for detection and validation of protein interactions. In addition, PepStar™ peptide microarray technology also enables enzyme profiling.
Chemical peptide synthesis is followed by peptide immobilization on glass slides via a flexible linker. Using chemoselective coupling, we generate peptide arrays displaying directed and covalently attached peptides. Multiple identical copies of each peptide microarray can be prepared with flexible layouts at unmatched prices and turnaround times.

Have a look at our incubation and assay services for epitope mapping and discovery!
Antibody Epitope Discovery Service  and Three-Step Seromarker Discovery Service.

Applications for PepStar™ Peptide Microarrays

  • Mapping of immunodominant regions in antigens
  • Antibody epitope mapping and optimization
  • Elucidation of signal transduction pathways
  • Identification of substrates for orphan enzymes
  • Optimization of known enzyme substrates
  • Reliable detection of contaminating enzyme activities

Application Notes:
The Challenge of Antigen Sequence Diversity: Solutions with ULTRA‐Peptide Libraries
by Pawlowski et al.
"Multiple Sclerosis and Epstein‐Barr Virus Infection. An Epitope Mapping Study"
by Reimer et al.
"Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic antibody IMAB362"
by Daneschar et al.
"Antibody Signatures Defined by High-Content Peptide Microarray Analyses"
by Schutkowski et al.
"A Modular Approach for Epitope Discovery and High-Resolution Profiling of Humoral Immune Responses"
by Pawlowski et al.

Benefits of PepStar™ Peptide Microarrays

Testimonials for PepStar™ Peptide Microarrays

"The RV 144 HIV trial is considered as one of first successful HIV vaccine trials. It has become clear that the V2 loop of gp120 is an important site for immunogenicity and protection from HIV infection. The use of JPT's PepStar™ microarray technology has been very useful for the correlation of the clincial outcome with humoral immune responses. As have the cyclic peptides been from JPT to validate these findings!"
Jeff Currier, PhD, Walter Reed Army Institute, Rockville, Maryland, USA

"Over the past five years my research team has been active in the development of species-specific peptide arrays. Our ability to advance this technology into new research areas has been dependent on working with the highest quality of arrays. In this regard, JPT’s commitment to excellence has been a critical foundation for the success of our research program.  I have been consistently impressed with their customer service, technical expertise and professionalism. When it comes to peptide synthesis there are no other options for our group…JPT is the best."
Scott Napper, PhD, Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Canada

More testimonials

Selected References for PepStar™ Peptide Microarrays

"Protective Efficacy of Multiple Vaccine Platforms Against Zika Virus Challenge in Rhesus Monkeys"
Abbink et al., Science (2016) - PMID: 27492477
"Anti-Hemagglutinin Antibody Derived Lead Peptides for Inhibitors of Influenza Virus Binding"
Memczak et al., PLoS One (2016) - PMID: 27415624
"Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases"
Klecker et al., Methods in molecular biology (2016) - PMID: 27424747
"Antibody Responses After Analytic Treatment Interruption in Human Immunodeficiency Virus-1-Infected Individuals on Early Initiated Antiretroviral Therapy"

Stephenson et al., Open Forum Infectious Diseases (2016) - PMID: n.a.
"Fine Specificity of the Antibody Response to Epstein-Barr Nuclear Antigen-2 and Other Epstein-Barr Virus Proteins in Patients with Clinically Isolated Syndrome: A Peptide Microarray-based Case-control Study"
Schlemm et al., Journal of Neuroimmunology  (2016) - PMID: n.a.
"Development of a Genus-Specific Antigen Capture ELISA for Orthopoxviruses–Target Selection and Optimized Screening"

Stern et al., PloS one (2016) - PMID: 26930499
"Superior Efficacy of an HIV Vaccine Combined with ARV Prevention in SHIV Challenged Non-human Primates"
Le Grand et al., Journal of Virology (2016) - PMID:  27009957
"High-Density Peptide Microarray Analysis of IgG Autoantibody Reactivities in Serum and Cerebrospinal Fluid of Multiple Sclerosis Patients"
Hecker et al., Mol. Cell. Proteomics. (2016) - PMID: 26831522
"Bayesian Hierarchical Modeling for Subject‐level Response Classification in Peptide Microarray Immunoassays"
Imholte et al., Biometrics (2016) - PMID: 27061097
"Structure of GPN-loop GTPase Npa3 and Implications for RNA Polymerase II Assembly "
Niesser t al., Mol Cell Biol. (2015) - PMID: 26711263
"Identification and Characterization of Common B Cell Epitope in Bovine Leukemia Virus via High-throughput Peptide Screening System in Infected Cattle"
Bai et al., Retrovirology (2015) - 26715158
"High-Throughput Microarray Incubations Using Multi-Well Chambers"
Zerweck et al., Methods Mol Biol. (2015) - PMID: 26490464
"Peptide Microarrays for Coverage of Sequence Diversity in Monitoring the B cell Immune Response (TECH2P.911)"
Castro, et al., The Journal of Immunology (2015) - PMID: n.a.
"Protective Efficacy of Adenovirus-protein Vaccines Against SIV Challenges in Rhesus Monkeys"
Barouch et al., Science (2015) - PMID: 26138104
"Relationships Between T Cell and IgE/IgG4 Epitopes of the Anisakis Simplex Major Allergen Ani s 1"
Alonso et al., Clin Exp Allergy. (2015) - PMID: 25495594
"H1N1 Viral Proteome Peptide Microarray Predicts Individuals at Risk for H1N1 Infection and Segregates Infection Versus Pandemrix® -Vaccination"
Aditya et al., Immunology (2015) - PMID: 25639813
"Antibodies to Influenza Nucleoprotein Cross-react with Human Hypocretin Receptor 2"
Ahmed et al., Science Translational Medicine (2015) - PMID: 26136476
"Serum Reactome Induced by Bordetella Pertussis Infection and Pertussis Vaccines: Qualitative Differences in Serum Antibody Recognition Patterns Revealed by Peptide Microarray Analysis"
Valentini et al., BMC Immunol. (2015) - PMID: 26129684
"Peptide Microarrays Enable Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic Antibody IMAB362"
Schnatbaum et al., Biotechnol J. (2014) - PMID: 24497417

Read More References with PepStar™ Peptide Microarrays

Related products and services

Three-Step Seromarker Profiling Service
Comprehensive and modular seromarker discovery workflow ranging from high throughput screening and parallel verification of peptide hits for hundreds of samples to validation of results by robust peptide ELISA
Epitope Discovery Service
Mapping of binding sites and antibody signature profiling using our PepStar™ peptide microarrays
RepliTope™ Catalog Microarrays
Peptide chips for mapping immunodominant regions in antigens
Kinase Peptide Microarrays
Compilation of kinase substrate peptides on ready-to-use PepStar peptide microarrays

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News Highlights

  • Analyze 21 Samples on a Single Microarray with our new RepliTope™ Multiwell Microarrays available for Tumor Antigens and Infections
    (August 2016)
  • Meet the Challenge of Sequence Diversity with ULTRA Peptide Libraries
    Learn more

    (December 2015)

--> Read more news

Quality Assurance

All production is performed according to ISO 9001:2015 standards

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