PepStar™ Peptide Microarrays

JPT's unique PepStar™ peptide microarrays are high-content or multiwell peptide arrays on glass slides. Peptide arrays (or peptide chips) are used for biomarker discovery, immunomonitoring, antibody epitope mapping, multiplexed epitope mapping or for detection and validation of protein interactions. In addition, PepStar™ peptide microarray technology also enables enzyme profiling.
Chemical peptide synthesis is followed by peptide immobilization on glass slides via a flexible linker. Using chemoselective coupling, we generate  peptide arrays displaying directed and covalently attached peptides. Multiple identical copies of each peptide microarray can be prepared with flexible layouts at unmatched prices and turnaround times.

Have a look at our incubation and assay services for epitope mapping and discovery!
Antibody Epitope Discovery Service  and Three-Step Seromarker Discovery Service.

Applications for PepStar™ Peptide Microarrays

  • Mapping of immunodominant regions in antigens
  • Antibody epitope mapping and optimization
  • Elucidation of signal transduction pathways
  • Identification of substrates for orphan enzymes
  • Optimization of known enzyme substrates
  • Reliable detection of contaminating enzyme activities

Application Notes:
The Challenge of Antigen Sequence Diversity: Solutions with ULTRA‐Peptide Libraries
by Pawlowski et al.
"Multiple Sclerosis and Epstein‐Barr Virus Infection. An Epitope Mapping Study"
by Reimer et al.
"Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic antibody IMAB362"
by Daneschar et al.
"Antibody Signatures Defined by High-Content Peptide Microarray Analyses"
by Schutkowski et al.
"A Modular Approach for Epitope Discovery and High-Resolution Profiling of Humoral Immune Responses"
by Pawlowski et al.

Benefits of PepStar™ Peptide Microarrays

Testimonials for PepStar™ Peptide Microarrays

"The RV 144 HIV trial is considered as one of first successful HIV vaccine trials. It has become clear that the V2 loop of gp120 is an important site for immunogenicity and protection from HIV infection. The use of JPT's PepStar™ microarray technology has been very useful for the correlation of the clincial outcome with humoral immune responses. As have the cyclic peptides been from JPT to validate these findings!"
Jeff Currier, PhD, Walter Reed Army Institute, Rockville, Maryland, USA

"Over the past five years my research team has been active in the development of species-specific peptide arrays. Our ability to advance this technology into new research areas has been dependent on working with the highest quality of arrays. In this regard, JPT’s commitment to excellence has been a critical foundation for the success of our research program.  I have been consistently impressed with their customer service, technical expertise and professionalism. When it comes to peptide synthesis there are no other options for our group…JPT is the best."
Scott Napper, PhD, Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Canada

More testimonials

Selected References for PepStar™ Peptide Microarrays

Anti-Hemagglutinin Antibody Derived Lead Peptides for Inhibitors of Influenza Virus Binding

Memczak et al., PLoS One (2016) - PMID: 27415624
Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases
Klecker et al., Methods in molecular biology (2016) - PMID: 27424747
Antibody Responses After Analytic Treatment Interruption in Human Immunodeficiency Virus-1-Infected Individuals on Early Initiated Antiretroviral Therapy

Stephenson et al., Open Forum Infectious Diseases (2016) - PMID: n.a.
Fine Specificity of the Antibody Response to Epstein-Barr Nuclear Antigen-2 and Other Epstein-Barr Virus Proteins in Patients with Clinically Isolated Syndrome: A Peptide Microarray-based Case-control Study
Schlemm et al., Journal of Neuroimmunology  (2016) - PMID: n.a.
Development of a Genus-Specific Antigen Capture ELISA for Orthopoxviruses–Target Selection and Optimized Screening

Stern et al., PloS one (2016) - PMID: 26930499
Superior Efficacy of an HIV Vaccine Combined with ARV Prevention in SHIV Challenged Non-human Primates
Le Grand et al., Journal of Virology (2016) - PMID:  27009957
High-Density Peptide Microarray Analysis of IgG Autoantibody Reactivities in Serum and Cerebrospinal Fluid of Multiple Sclerosis Patients
Hecker et al., Mol. Cell. Proteomics. (2016) - PMID: 26831522
Bayesian Hierarchical Modeling for Subject‐level Response Classification in Peptide Microarray Immunoassays
Imholte et al., Biometrics (2016) - PMID: 27061097
Structure of GPN-loop GTPase Npa3 and Implications for RNA Polymerase II Assembly
Niesser t al., Mol Cell Biol. (2015) - PMID: 26711263
Identification and Characterization of Common B Cell Epitope in Bovine Leukemia Virus via High-throughput Peptide Screening System in Infected Cattle
Bai et al., Retrovirology (2015) - 26715158
High-Throughput Microarray Incubations Using Multi-Well Chambers
Zerweck et al., Methods Mol Biol. (2015) - PMID: 26490464
Peptide Microarrays for Coverage of Sequence Diversity in Monitoring the B cell Immune Response (TECH2P.911)
Castro, et al., The Journal of Immunology (2015) - PMID: n.a.
Protective Efficacy of Adenovirus-protein Vaccines Against SIV Challenges in Rhesus Monkeys
Barouch et al., Science (2015) - PMID: 26138104
Relationships Between T Cell and IgE/IgG4 Epitopes of the Anisakis Simplex Major Allergen Ani s 1
Alonso et al., Clin Exp Allergy. (2015) - PMID: 25495594
H1N1 Viral Proteome Peptide Microarray Predicts Individuals at Risk for H1N1 Infection and Segregates Infection Versus Pandemrix® -Vaccination
Aditya et al., Immunology (2015) - PMID: 25639813
Antibodies to Influenza Nucleoprotein Cross-react with Human Hypocretin Receptor 2
Ahmed et al., Science Translational Medicine (2015) - PMID: 26136476
Serum Reactome Induced by Bordetella Pertussis Infection and Pertussis Vaccines: Qualitative Differences in Serum Antibody Recognition Patterns Revealed by Peptide Microarray Analysis
Valentini et al., BMC Immunol. (2015) - PMID: 26129684
Peptide Microarrays Enable Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic Antibody IMAB362
Schnatbaum et al., Biotechnol J. (2014) - PMID: 24497417

Read More References with PepStar™ Peptide Microarrays

Related products and services

Three-Step Seromarker Profiling Service
Comprehensive and modular seromarker discovery workflow ranging from high throughput screening and parallel verification of peptide hits for hundreds of samples to validation of results by robust peptide ELISA
Epitope Discovery Service
Mapping of binding sites and antibody signature profiling using our PepStar™ peptide microarrays
RepliTope™ Catalog Microarrays
Peptide chips for mapping immunodominant regions in antigens
Kinase Peptide Microarrays
Compilation of kinase substrate peptides on ready-to-use PepStar peptide microarrays

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News Highlights

  • Analyze 21 Samples on a Single Microarray with our new RepliTope™ Multiwell Microarrays available for Tumor Antigens and Infections
    (August 2016)
  • Meet the Challenge of Sequence Diversity with ULTRA Peptide Libraries
    Learn more

    (December 2015)

--> Read more news

Quality Assurance

All production is performed according to ISO 9001:2015 standards

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