PepStar™ Peptide Microarrays

JPT Peptide Technologies applies its unique PepStar™ peptide microarray platform to generate high-content and multiwell peptide microarrays on glass slides. Peptide arrays (or peptide chips) are used for biomarker discovery, immunomonitoring, antibody epitope mapping, multiplexed epitope mapping or for detection and validation of protein interactions. In addition, PepStar™ peptide microarray technology also enables enzyme profiling.
Chemical peptide synthesis is followed by peptide immobilization on glass slides via a flexible linker. Using chemoselective coupling, we generate microarrays displaying directed and covalently attached peptides. Multiple identical copies of each peptide microarray can be prepared with flexible layouts at unmatched prices and turnaround times.

Have a look at our incubation and assay services!
Antibody Epitope Discovery Service  and Three-Step Seromarker Discovery Service.

Applications for PepStar™ Peptide Microarrays

  • Mapping of immunodominant regions in antigens
  • Identification of substrates for orphan enzymes
  • Optimization of known enzyme substrates
  • Elucidation of signal transduction pathways
  • Reliable detection of contaminating enzyme activities

Application Notes:
"Multiple Sclerosis and Epstein‐Barr Virus Infection. An Epitope Mapping Study"
by Reimer et al.
"Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic antibody IMAB362"
by Daneschar et al.
"Antibody Signatures Defined by High-Content Peptide Microarray Analyses"
by Schutkowski et al.
"A Modular Approach for Epitope Discovery and High-Resolution Profiling of Humoral Immune Responses"
by Pawlowski et al.

Benefits of PepStar™ Peptide Microarrays

Testimonials for PepStar™ Peptide Microarrays

"The RV 144 HIV trial is considered as one of first successful HIV vaccine trials. It has become clear that the V2 loop of gp120 is an important site for immunogenicity and protection from HIV infection. The use of JPT's PepStar™ microarray technology has been very useful for the correlation of the clincial outcome with humoral immune responses. As have the cyclic peptides been from JPT to validate these findings!"
Jeff Currier, PhD, Walter Reed Army Institute, Rockville, Maryland, USA

"Over the past five years my research team has been active in the development of species-specific peptide arrays. Our ability to advance this technology into new research areas has been dependent on working with the highest quality of arrays. In this regard, JPT’s commitment to excellence has been a critical foundation for the success of our research program.  I have been consistently impressed with their customer service, technical expertise and professionalism. When it comes to peptide synthesis there are no other options for our group…JPT is the best."
Scott Napper, PhD, Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Canada

More testimonials

Selected References for PepStar™ Peptide Microarrays

High-Throughput Microarray Incubations Using Multi-Well Chambers
Zerweck et al., Methods Mol Biol. (2015) - PMID: 26490464
Peptide Microarrays for Coverage of Sequence Diversity in Monitoring the B cell Immune Response (TECH2P.911)
Castro, et al., The Journal of Immunology (2015) - PMID: n.a.
Protective Efficacy of Adenovirus-protein Vaccines Against SIV Challenges in Rhesus Monkeys
Barouch et al., Science (2015) - PMID: 26138104
Relationships Between T Cell and IgE/IgG4 Epitopes of the Anisakis Simplex Major Allergen Ani s 1
Alonso et al., Clin Exp Allergy. (2015) - PMID: 25495594
H1N1 Viral Proteome Peptide Microarray Predicts Individuals at Risk for H1N1 Infection and Segregates Infection Versus Pandemrix® -Vaccination
Aditya et al., Immunology (2015) - PMID: 25639813
Peptide Microarrays Enable Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic Antibody IMAB362
Schnatbaum et al., Biotechnol J. (2014) - PMID: 24497417
Peanut Oral Immunotherapy Modifies IgE and IgG4 Responses to Major Peanut Allergens
Vickery et al., J Allergy Clin Immunol. (2013) - PMID: 23199605
Plasma IgG to Linear Epitopes in the V2 and V3 Regions of HIV-1 gp120 Correlate with a Reduced Risk of Infection in the RV144 Vaccine Efficacy Trial
Gottardo et al., PLoS One. (2013) - PMID: 24086607
Nonreplicating Vaccines Can Protect African Green Monkeys From the Memphis 37 Strain of Respiratory Syncytial Virus

Eyles et al., The Journal of Infectious Disease (2013) - PMID: 23596321
Serum Autoantibodies to Myelin Peptides Distinguish Acute Disseminated Encephalomyelitis From relapsing– Remitting Multiple Sclerosis

Van Haren et al., Multiple Sclerosis Journal (2013) - PMID: 23612879
Neutralization of Porcine Endogenous Retrovirus by Antibodies Against the Membrane-Proximal External Region of the Transmembrane Envelope Protein

Wächter et al., Journal of General Virology (2013) - PMID: 23223617
Characterization of Humoral and Cellular Immune Responses Elicited by a Recombinant Adenovirus Serotype 26 HIV-1 Env Vaccine in Healthy Adults (IPCAVD 001)

Barouch et al., J. Immunol. (2013) - PMID: 23125443

More references under JPT Publications/Literature

Related products and services

Three-Step Seromarker Profiling Service
Comprehensive and modular seromarker discovery workflow ranging from high throughput screening and parallel verification of peptide hits for hundreds of samples to validation of results by robust peptide ELISA
Epitope Discovery Service
Mapping of binding sites and antibody signature profiling using our PepStar™ peptide microarrays
RepliTope™ Catalog Microarrays
Peptide chips for mapping immunodominant regions in antigens
Kinase Peptide Microarrays
Compilation of kinase substrate peptides on ready-to-use PepStar peptide microarrays

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News Highlights

Meet the Challenge of Sequence Diversity with ULTRA Peptide Libraries
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(December 2015)
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Quality Assurance

All production is performed according to ISO 9001:2008 standards