PepSpots™ Peptide Arrays on Cellulose
The SPOT peptide synthesis was developed to give rapid and low-cost access to large numbers of custom peptides.
They are used both as membrane bound peptide arrays (PepSpots™) for efficient and inexpensive antibody epitope mapping or protein-protein interaction studies and as soluble peptide libraries (Micro-Scale Peptides and PepTrack™ Peptide Libraries).
Properties of PepSpots™ Membrane Arrays:
- Membrane-bound peptides synthesized directly on cellulose membranes by SPOT synthesis technology (SPOT peptide synthesis technique)
- SPOT synthesis is a fast and economic technique for custom peptide synthesis of hundreds of membrane peptides in parellel
- Membrane-bound peptide arrays (PepSpots™) are used for efficient and inexpensive antibody epitope mapping or protein-protein interaction studies.
- Easy experimental procedure (like ELISA), inexpensive equipment and flexible array and library formats
The method of choice for fast and reliable antibody epitope mapping and characterization are PepSpots™ Membrane Arrays. However, for high troughput screening with thousands of peptides and projects with unpurified samples (e.g. serum, lysate, blood) we recommend the use of PepStar™ Peptide Microarrays. Our customer support will be glad to help in case of question (firstname.lastname@example.org).
Comparison of PepSpots™ Peptides on Cellulose and PepStar™ Peptide Microarrays
|Peptides attached via||C-terminus||N-terminus|
|Reorder possible from same synthesis||No||Yes, up to several hundred|
|Immobilization of protein controls possible||No||Yes, up to 8 controls|
|Total incubation volume of diluted sample||Depending on number of peptides (several ml)||200 - 300 µl|
|Applicable to patient samples||Limited||Yes|
|Each peptide is displayed||1x||3x|
|Array surface||Cellulose membrane||Glass slide|
Applications for PepSpots™ Peptides on Cellulose
- Antibody epitope mapping
- Functional characterization of mapped epitopes (e.g substitutional or truncation analyses):
Which amino acids are critical for binding?
Which amino acids are so called "key-residues”?
- Characterization of protein-protein contact sites, such as:
- Protein modules involved in signal transduction processes
- Recognition sequences for heat shock proteins
Benefits of PepSpots™ Peptides on Cellulose
- Rapid, low-cost and accurate synthesis using robotics (SPOT Technology)
- Hydrophilic surface of cellulose membranes
- Minimization of unspecific hydrophobic interactions with target molecule
- Easy detection using standard ELISA protocols
Testimonials for PepSpots™ Peptides on Cellulose
"Our teams at the Vrije Universiteit Brussel deal with a wide variety of projects in different areas of applied biological sciences. For many of them we are in need of peptide related tools and services ranging from peptide libraries and highly purified peptides to peptide arrays. In our search for an integrated and reliable provider we came across JPT. Meanwhile, we are collaborating with JPT for many years and are very satisfied with the relationship which led to several well received publications. Especially, their unique array technology helped us to enhance our knowledge on different protein protein interactions such as Chaperone-substrate recognition."
Prof. Joost W.H. Schymkowitz & Prof. Frederic Rousseau, VIB Switch Laboratory, Vrije Universiteit Brussel, Belgium
Selected References for PepSpots™ Peptides on Cellulose
"Therapeutic Anti-IgE Monoclonal Antibody Single Chain Variable Fragment (scFv) Safety and Immunomodulatory Effects After One Time Injection in Four Dogs"
Hammerberg et al., Veterinary Dermatology (2016) - PMID: 27426720
"Defining Functional Classes of Barth Syndrome Mutation in Humans"
Lu et al., Hum Mol Genet. (2016) - PMID: 26908608
"Optimization of the All-D Peptide D3 for Aβ Oligomer Elimination"
Klein et al., PLoS One (2016) - PMID: 27105346
"Solid-material-based Coupling Efficiency Analyzed with Time-of-Flight Secondary Ion Mass Spectrometry "
Muenster et al, Applied Surface Science (2015)- PMID: n.a.
"Development of a Rapid and Simple Immunochromatographic Assay to Identify Vibrio Parahaemolyticus"
Sakata et al., J Microbiol Methods. (2015) - PMID: 26093260
"Itch WW Domains Inhibit its E3 Ubiquitin Ligase Activity by Blocking E2-E3 Transthiolation"
Riling et al., J Biol Chem. (2015) - PMID: 26245901
"Cardiac Myosin-binding Protein C: a Potential Early Biomarker of Myocardial Injury"
Baker et al., Basic Res Cardiol. (2015) - PMID: 25837837
"Identification of Amyloid Beta Mid-domain Fragments in Human Cerebrospinal Fluid"
Rogeberg et al., Biochimie (2015) - PMID: 25866191
"Differential and Concordant Roles for PARP1 and Poly (ADP-ribose) in Regulating WRN and RECQL5 Activities"
Khadka et al., Mol Cell Biol. (2015) - PMID: 26391948
"Structural Differences of Amyloid-β fibrils Revealed by Antibodies From Phage Display"
Droste et al., BMC Biotechnol. (2015) - PMID: 26084577
Read More References with PepSpots™ Peptides on Cellulose
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