PepSpots™ Peptide Arrays on Cellulose

The peptides on PepSpots™ Peptide Arrays are synthesized directly on cellulose membranes by SPOT synthesis technology (SPOT peptide synthesis technique). SPOT synthesis is a fast and economic technique for custom peptide synthesis of hundreds of membrane peptides in parellel. SPOT peptide synthesis was developed to give rapid and low-cost access to large numbers of custom peptides.
They are used both as membrane bound peptide arrays (PepSpots™) for efficient and inexpensive antibody epitope mapping or protein-protein interaction studies and as soluble peptide libraries (Micro-Scale Peptides and PepTrack™ Peptide Libraries). PepSpots™ peptide arrays are reliable and combine an easy experimental procedure (like ELISA), inexpensive equipment needs and a highly flexible array and library formatting.
More than 100 scientific papers describing the application of this PepSpots™ peptide array technology and the hundreds of successful epitope mapping and epitope characterization projects performed at JPT over the last decade distinguish PepSpot™ peptide arrays as the method of choice for fast and reliable antibody epitope mapping and characterization.

For high troughput screening with thousands of peptides and projects with unpurified samples (e.g. serum, lysate, blood) we recommend the use of PepStar™ Peptide Microarrays. See the table below for a comparison of both technologies. Our customer support will be glad to help in case of question (peptide@jpt.com).

Comparison of PepSpots™ Peptides on Cellulose and PepStar™ Peptide Microarrays

PepSpots™ PepStar™
Peptides attached via C-terminus N-terminus
Peptides purified No Yes
Read-out via Chemiluminescence Fluorescence
Regeneration possible Yes No
Reorder possible from same synthesis No Yes, up to several hundred
Immobilization of protein controls possible No Yes, up to 8 controls
Total incubation volume of diluted sample Depending on number of peptides (several ml) 200 - 300 µl
Applicable to patient samples Limited Yes
Each peptide is displayed 1x 3x
Array surface Cellulose membrane Glass slide

Applications for PepSpots™ Peptides on Cellulose

  • Antibody epitope mapping
  • Functional characterization of mapped epitopes (e.g substitutional or truncation analyses):
    Which amino acids are critical for binding?
    Which amino acids are so called "key-residues”?
  • Characterization of protein-protein contact sites, such as:
    • Receptor-ligand
    • Enzyme-substrate
    • Protein modules involved in signal transduction processes
    • Recognition sequences for heat shock proteins

Benefits of PepSpots™ Peptides on Cellulose

  • Rapid, low-cost and accurate synthesis using robotics (SPOT Technology)
  • Hydrophilic surface of cellulose membranes
  • Minimization of unspecific hydrophobic interactions with target molecule
  • Easy detection using standard ELISA protocols

Testimonials for PepSpots™ Peptides on Cellulose

"Our teams at the Vrije Universiteit Brussel deal with a wide variety of projects in different areas of applied biological sciences. For many of them we are in need of peptide related tools and services ranging from peptide libraries and highly purified peptides to peptide arrays. In our search for an integrated and reliable provider we came across JPT. Meanwhile, we are collaborating with JPT for many years and are very satisfied with the relationship which led to several well received publications. Especially, their unique array technology helped us to enhance our knowledge on different protein protein interactions such as Chaperone-substrate recognition."
Prof. Joost W.H. Schymkowitz & Prof. Frederic Rousseau, VIB Switch Laboratory, Vrije Universiteit Brussel, Belgium

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Selected References for PepSpots™ Peptides on Cellulose

Structural Differences of Amyloid-β fibrils Revealed by Antibodies From Phage Display
Droste et al., BMC Biotechnol. (2015) - PMID: 26084577
Arg Kinase Binding Protein 2 (ArgBP2) Interaction With α-actinin and Actin Stress Fibres Inhibits Cell Migration
Anekal et al., J. Biol. Chem. (2014) - PMID: 25429109
Intracellular Amyloid and the Neuronal Origin of Alzheimer Neuritic Plaques
Pensalfini et al. Neurobiology of Disease (2014) - PMID: 25092575
MageA3 Binding Antibodies
Esslinger et al., US Patent 20,140,186,363, (2014)
Modulation of the Chromatin Phosphoproteome by the Haspin Protein Kinase
Maiolica et al., Molecular et Cellular Proteomics (2014) - PMID: 24732914
Use of YSCF, Truncated YSCF and YSCF Homologs as Adjuvants
Nilles et al., US Patent 20,140,120,128, (2014) - PMID: n.a.
SHP-2 Binds to Caveolin-1 and Regulates Src Activity via Competitive Inhibition of CSK in Response to H2O2 in Astrocytes
Jo et al., PLoS One (2014) - PMID: 24632732
The N-Terminal Methionine of Cellular Proteins as a Degradation Signal
Kim et al., Cell. (2014) - PMID: 24361105
Targeting a Cross-Reactive Gly m 5 Soy Peptide as Responsible for Hypersensitivity Reactions in a Milk Allergy Mouse Model
Curciarello et al., PLoS One (2014) - PMID: 24416141

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