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PepMix™ Peptide Pools

Overlapping peptide pools (peptide mixes of overlapping peptide scans from virus or tumor antigens such as CMV peptide pools, HPV peptide pools or HIV peptide pools) or mixed antigenic peptide pools (e.g. CEFX Ultra SuperStim & CEF Pool with antigens from CMV, EBV, Influenza and others) are extremely efficient for antigen-specific T-cell stimulation in T cell assays, T-cell expansion, immune monitoring, cellular immune response profiling or T-cell epitope identification.
In addition to overlapping peptide pools that covers entire antigens, we offer selected antigen peptide pools such as CEFX Ultra SuperStim Pool, CEF Pool, EF Pool and CEFT Pools. Peptide Pools can also be used to standardize T-cell assays, as synthetic pooled peptides are much more robust and reliable for T-cell stimulation (e.g. in ELISPOT, ICS, cytotoxity or proliferation assays) than proteins or lysates.

Custom Peptide Pools & Matrix Pools

Custom PepMixes™ Peptide Pools

You need a specific antigen, a set of neo epitopes or a specialized peptide pool layout?
We are happy to discuss your specifications and provide your tailored PepMix™, neo epitope pools or matrix pools. Our customer support team will assist you with peptide scans, sequence and pool design. Subsequently, we will chemically synthesize, purify and analyze the peptides in compliance with the requirements of T-cell assays. All peptide synthesis steps are optimized to avoid contaminations and failure sequences which may lead to false positive T-cell responses or inhibition of T-cell responses.
Pooling and aliquotation are performed using our validated protocols to ensure high quality purified pooled peptides in freeze dried aliquots for long stability.

Custom PepMix™ Production

Control Peptide Pools for T-cell Assays

PM-CEFX-1CEFX Ultra SuperStim Pool Positive Control Pool of 176 known peptide epitopes for a broad range of HLA sub-types and different infectious agents for T-cell stimulation of populations with a diverse ethnic backgroundInfo
PM-EFX-1EFX Ultra SuperStim Pool Positive Control Pool of 147 known peptide epitopes for a broad range of HLA sub-types and different infectious agents for T-cell stimulation of populations with a diverse ethnic background. No epitopes originating from HHV5 (CMV) are includedInfo
PM-CEF-E-2CEF Pool (extended) (120nmol; >70%) Positive Control Pool of 32 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-CEF-E-4CEF Pool (extended) (120nmol; >90%) Positive Control Pool of 32 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-CEF-E-1CEF Pool (extended) (15nmol; >70%) Positive Control Pool of 32 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-CEF-E-3CEF Pool (extended) (15nmol; >90%) Positive Control Pool of 32 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-CEF-S-2CEF Pool (standard) (120nmol; >70%) Positive Control Pool of 23 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-CEF-S-4CEF Pool (standard) (120nmol; >90%) Positive Control Pool of 23 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (antigen specific T-cell stimulation)Info
PM-CEF-S-1CEF Pool (standard) (15nmol; >70%) Positive Control Pool of 23 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-CEF-S-3CEF Pool (standard) (15nmol; >90%) Positive Control Pool of 23 peptides peptides selected from defined HLA class I-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-CEFT-MHC-II-2CEFT MHC-II Pool (120nmol; >70%) Pool of 14 peptides peptides selected from defined HLA class II-restricted T-cell epitopes for T cell assays (e.g. antigen specific T-cell stimulation)Info
PM-CEFT-MHC-II-4CEFT MHC-II Pool (120nmol; >90%) Pool of 14 peptides peptides selected from defined HLA class II-restricted T-cell epitopes for T cell assays (e.g. antigen specific T-cell stimulation)Info
PM-CEFT-MHC-II-1CEFT MHC-II Pool (15nmol; >70%) Pool of 14 peptides peptides selected from defined HLA class II-restricted T-cell epitopes for T cell assays (e.g. antigen specific T-cell stimulation)Info
PM-CEFT-MHC-II-3CEFT MHC-II Pool (15nmol; >90%) Pool of 14 peptides peptides selected from defined HLA class II-restricted T-cell epitopes for T cell assays (e.g. antigen specific T-cell stimulation)Info
PM-CEFT-2CEFT Pool (120nmol; >70%) Positive Control Pool of 27 peptides peptides selected from defined HLA class I and II-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-CEFT-4CEFT Pool (120nmol; >90%) Positive Control Pool of 27 peptides peptides selected from defined HLA class I and II-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-CEFT-1CEFT Pool (15nmol; >70%) Positive Control Pool of 27 peptides peptides selected from defined HLA class I and II-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-CEFT-3CEFT Pool (15nmol; >90%) Positive Control Pool of 27 peptides peptides selected from defined HLA class I and II-restricted T-cell epitopes for T cell assays (e.g. ELISPOT)Info
PM-ACTSPepMix™ Human (Actin) Negative Control Pool of 92 peptides derived from a peptide scan through Actin, alpha skeletal muscle of Homo sapiens (Human) for T cell assaysInfo
PM-C1PepMix™ Human (HLA classI Ig-like C1 type domain) Negative Control Pool of 19 peptides derived from a peptide scan through HLA class I histocompatibility antigen, A-36 alpha chain of Homo sapiensInfo
PM-MOGPepMix™ Human (MOG) Negative Control Pool of 29 peptides derived from a peptide scan through Myelin-oligodendrocyte glycoprotein (MOG) of Homo sapiens (Human)Info

Peptide Pools for Infectious Diseases Antigens

PM-HPV18-E6PepMix™ HPV 18 (Protein E6) Pool of 37 peptides derived from a peptide scan through Protein E6 of Human papillomavirus type 18 for T cell assays (e.g. ELISPOT)Info
PM-AAV8-CPPepMix AAV8 (capsid protein) Pool of 182 peptides derived from a peptide scan (15mers with 11 aa overlap) through Capsid protein (UniProt ID: Q8JQF8) of Adeno-associated virus 8Info
PM-AAV6-VP1PepMix™ AAV6 (Capsid protein VP1) Pool of 182 overlapping peptides from Capsid protein VP1 of Adeno-associated virus 6 for T cell assays (antigen specific T-cell stimulation)Info
PM-BKV-VP1PepMix™ BKV (capsid protein VP1) Pool of 88 peptides derived from a peptide scan through Major capsid protein VP1 of BK polyomavirus for T cell assays (e.g. ELISPOT)Info
PM-BKV-VP2PepMix™ BKV (capsid protein VP2) Pool of 85 peptides derived from a peptide scan through Minor capsid protein VP2 of BK polyomavirus for T cell assays (e.g. ELISPOT)Info
PM-BKV-VP3PepMix™ BKV (capsid protein VP2, isoform VP3) Pool of 56 peptides derived from a peptide scan through Minor capsid protein VP2, isoform VP3 of BK polyomavirus for T cell assaysInfo
PM-BKV-LTAPepMix™ BKV (large T antigen) Pool of 170 peptides derived from a peptide scan through Large T antigen of BK polyomavirus for T cell assays (antigen specific T-cell stimulation)Info
PM-BKV-STAPepMix™ BKV (small T antigen) Pool of 41 peptides derived from a peptide scan through Small t antigen of BK polyomavirus for T cell assays (e.g. antigen specific T-cell stimulation)Info
PM-CAND-MP65PepMix™ Candida (MP65) Pool of 92 peptides derived from a peptide scan through Mannoprotein MP65 of Candida albicans (Yeast) for T cell assays (e.g. ELISPOT)Info
PM-C-EBV-1PepMix™ Collection EBV (2x1ug/peptide) Collection of 14 peptide pools through 14 immunodominant antigens of Epstein-Barr virus (EBV)Info
PM-C-HCMV-1PepMix™ Collection HCMV (2x1ug/peptide) Collection of 19 peptide pools derived from scans through 19 immuonodominant antigens of Human cytomegalovirus (HHV-5)Info
PM-CyCMV-UL55PepMix™ CyCMV (GP-UL55) Pool of 210 peptides derived from a peptide scan through Glycoprotein UL55 of Cynomolgus macaque cytomegalovirus for T cell assays (e.g. ELISPOT)Info
PM-CyCMV-UL83PepMix™ CyCMV (TP-UL83) Pool of 133 peptides derived from a peptide scan through Tegument phosphoprotein UL83 of Cynomolgus macaque cytomegalovirus for T cell assays (e.g. ELISPOT)Info
PM-EBV-BARF1PepMix™ EBV (BARF1) Pool of 53 overlapping peptides from Secreted protein BARF1 (p53) of Epstein-Barr virus (EBV) for T cell assays (e.g. ELISPOT)Info
PM-EBV-BMLF1PepMix™ EBV (BMLF1) Pool of 117 peptides derived from a peptide scan through mRNA export factor EB2 (BMLF1) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-EBV-BMRF1PepMix™ EBV (BMRF1) Pool of 99 peptides derived from a peptide scan through DNA polymerase processivity factor BMRF1 of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-EBV-BRLF1PepMix™ EBV (BRLF1) Pool of 149 peptides derived from a peptide scan through Transcription activator BRLF1 of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-EBV-BZLF1PepMix™ EBV (BZLF1) Pool of 59 peptides derived from a peptide scan through Trans-activator protein BZLF1 of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-EBV-EBNA-LPPepMix™ EBV (EBNA-LP) Pool of 124 peptides derived from a peptide scan through Epstein-Barr nuclear antigen leader protein (EBNA-LP) of Epstein-Barr virus (HHV4)Info
PM-EBV-EBNA1PepMix™ EBV (EBNA1) Pool of 158 peptides derived from a peptide scan through Epstein-Barr nuclear antigen 1 (EBNA1) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-EBV-EBNA2PepMix™ EBV (EBNA2) Pool of 119 peptides derived from a peptide scan through Epstein-Barr nuclear antigen 2 (EBNA2) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-EBV-EBNA3aPepMix™ EBV (EBNA3a) Pool of 234 peptides derived from a peptide scan through Epstein-Barr nuclear antigen 3 (EBNA3a) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-EBV-EBNA3bPepMix™ EBV (EBNA3b) Pool of 279 peptides derived from a peptide scan through Epstein-Barr nuclear antigen 4 (EBNA3b) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-EBV-EBNA3cPepMix™ EBV (EBNA3c) Pool of 265 peptides derived from a peptide scan through Epstein-Barr nuclear antigen 6 (EBNA3c) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-EBV-GP350/GP340PepMix™ EBV (GP350/GP340) Pool of 224 peptides derived from a peptide scan (15mers with 11 aa overlap) through Envelope glycoprotein GP350/GP340 of Epstein-Barr virus (HHV4)Info
PM-EBV-LMP1PepMix™ EBV (LMP1) Pool of 94 peptides derived from a peptide scan through Latent membrane protein 1 (LMP1) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-EBV-LMP2PepMix™ EBV (LMP2) Pool of 122 peptides derived from a peptide scan through Latent membrane protein 2 (LMP2) of Epstein-Barr virus (HHV4) for T cell assaysInfo
PM-FTUL-OMPPepMix™ F. tularensis (Omp) Pool of 45 peptides derived from a peptide scan through Outer membrane protein of Francisella tularensis subsp. tularensis for T cell assays (e.g. ELISPOT)Info
PM-HAdV3PepMix™ HAdV-3 (hexon protein) Pool of 234 peptides derived from a peptide scan through Hexon protein of Human adenovirus 3 (HAdV-3) for T cell assays (e.g. ELISPOT)Info
PM-HAdV5PepMix™ HAdV-5 (penton protein) Pool of 140 peptides derived from a peptide scan through Penton protein of Human adenovirus 5 (HAdV-5) for T cell assays (e.g. ELISPOT)Info
PM-HBV-CPPepMix™ HBV (Capsid protein) Pool of 44 peptides derived from a peptide scan through Capsid protein of Hepatitis B virus (HBV-A) for T cell assays (e.g. ELISPOT)Info
PM-HBV-CPULTRAPepMix™ HBV (Capsid Protein) Ultra Pool of 155 peptides derived from a peptide scan through Capsid protein of HBV for T cell assays (e.g. ELISPOT)Info
PM-HBV-LEPPepMix™ HBV (Large envelope protein) Pool of 98 peptides derived from a peptide scan through Large envelope protein of Hepatitis B virus (HBV-A) for T cell assays (e.g. ELISPOT)Info
PM-IE1PepMix™ HCMVA (IE-1) Positive Control Pool of 120 peptides derived from a peptide scan through 55 kDa immediate-early protein 1 (IE-1) of Human cytomegalovirus (HHV-5)Info
PM-IE2PepMix™ HCMVA (IE-2) Positive Control Pool of 143 peptides derived from a peptide scan through 45 kDa immediate-early protein 2 (IE-2) of Human cytomegalovirus (HHV-5).Info
PM-PP65-1PepMix™ HCMVA (pp65) (>70%) Positive Control Pool of 138 peptides derived from a peptide scan through 65 kDa phosphoprotein (pp65) of Human cytomegalovirus (HHV-5)Info
PM-PP65-2PepMix™ HCMVA (pp65) (>90%) Positive Control Pool of 138 peptides derived from a peptide scan through 65 kDa phosphoprotein (pp65) of Human cytomegalovirus (HHV-5)Info
PM-HIV-CONBPepMix™ HIV-1 (Con B gag motif) Negative Control Pool of 123 peptides selected from Con B gag motifs of Human immunodeficiency virus strainsInfo
PM-UL103PepMix™ HCMVA (UL103) Pool of 60 peptides derived from a peptide scan through Protein UL103 of Human cytomegalovirus (HHV-5) for T cell assays (e.g. ELISPOT)Info
PM-UL151PepMix™ HCMVA (UL151) Pool of 82 peptides derived from a peptide scan through Orf UL151 of Human cytomegalovirus (HHV-5) for T cell assays (e.g. ELISPOT)Info
PM-UL153PepMix™ HCMVA (UL153) Pool of 67 peptides derived from a peptide scan through Orf UL153 of Human cytomegalovirus (HHV-5) for T cell assays (e.g. ELISPOT)Info
PM-UL28PepMix™ HCMVA (UL28) Pool of 92 peptides derived from a peptide scan through Uncharacterized protein UL28 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-UL32PepMix™ HCMVA (UL32) Pool of 260 peptides derived from a peptide scan through Large structural phosphoprotein (UL32) of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-UL36PepMix™ HCMVA (UL36) Pool of 117 peptides derived from a peptide scan through Uncharacterized protein UL36 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-UL40PepMix™ HCMVA (UL40) Pool of 53 peptides derived from a peptide scan through Uncharacterized protein UL40 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-UL48PepMix™ HCMVA (UL48)_ 2 subpools Pool of 2 x 281 peptides derived from a peptide scan through Deneddylase UL48 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-UL55PepMix™ HCMVA (UL55) Pool of 224 peptides derived from a peptide scan through Envelope glycoprotein B (UL55) of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-UL82PepMix™ HCMVA (UL82) Pool of 137 peptides derived from a peptide scan through 71 kDa phosphoprotein (UL82) of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-UL94PepMix™ HCMVA (UL94) Pool of 84 peptides derived from a peptide scan through Capsid-binding protein UL94 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-UL99PepMix™ HCMVA (UL99) Pool of 45 peptides derived from a peptide scan through Tegument protein UL99 of Human cytomegalovirus (HHV-5) for T cell assays (e.g. ELISPOT)Info
PM-US24PepMix™ HCMVA (US24) Pool of 123 peptides derived from a peptide scan through Tegument protein US24 of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-US29PepMix™ HCMVA (US29) Pool of 113 peptides derived from a peptide scan through Uncharacterized protein HHRF4 (US29) of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-US3PepMix™ HCMVA (US3) Pool of 44 peptides derived from a peptide scan through Unique short US3 glycoprotein of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-US32PepMix™ HCMVA (US32) Pool of 43 peptides derived from a peptide scan through Uncharacterized protein HHRF7 (US32) of Human cytomegalovirus (HHV-5) for T cell assaysInfo
PM-HEV-ORF2PepMix™ HEV (ORF2) Pool of 163 peptides derived from a peptide scan (15mers with 11 aa overlap) through Capsid protein (UniProt ID: E9N3B9) of Hepatitis E virus (strain Kernow-C1)Info
PM-HEV-ORF3PepMix™ HEV (ORF3) Pool of 26 peptides derived from a peptide scan (15mers with 11 aa overlap) through Uncharacterized protein ORF 3 (UniProt ID: E9N3B8) of Hepatitis E virus (strain Kernow-C1)Info
PM-HHV6-U54PepMix™ HHV6 (U54) Pool of 112 peptides derived from a peptide scan through U54 of Human herpesvirus 6B for T cell assays (antigen specific T-cell stimulation)Info
PM-HHV6-U90PepMix™ HHV6 (U90) Pool of 267 peptides derived from a peptide scan through Protein U90 of Human herpesvirus 6B for T cell assays (antigen specific T-cell stimulation)Info
PM-HIV-ENVPepMix™ HIV (ENV) Ultra Pool of 150 15meric peptides derived from Envelope glycoprotein gp160 designed to cover the high sequence diversity of the HI virus for T cell assaysInfo
PM-HIV-GAGPepMix™ HIV (GAG) Ultra Pool of 150 15meric peptides derived from Gag polyprotein designed to cover the high sequence diversity of the HIV virus for T cell assays (e.g. ELISPOT)Info
PM-HIV-NEFPepMix™ HIV (NEF) Ultra Pool of 150 15meric peptides derived from Nef protein designed to cover the high sequence diversity of the HI virus for T cell assays (e.g. ELISPOT)Info
PM-HIV-POLPepMix™ HIV (POL) Ultra Pool of 150 15meric peptides derived from Pol Polyprotein designed to cover the high sequence diversity of the HI virus for T cell assaysInfo
PM-HPV16-E6PepMix™ HPV 16 (Protein E6) Pool of 37 peptides derived from a peptide scan through Protein E6 of Human papillomavirus type 16 for T cell assays (e.g. ELISPOT)Info
PM-HPV16-E7PepMix™ HPV 16 (Protein E7) Pool of 22 peptides derived from a peptide scan through Protein E7 of Human papillomavirus type 16 for T cell assays (e.g. ELISPOT)Info
PM-HPV18-E7PepMix™ HPV 18 (Protein E7) Pool of 24 peptides derived from a peptide scan through Protein E7 of Human papillomavirus type 18 for T cell assays (e.g. ELISPOT)Info
PM-HPV16-L1PepMix™ HPV16 (L1) Pool of 124 overlapping peptides from Late major capsid protein L1 (L1) of Human papillomavirus type 16 (HPV) for T cell assays (e.g. ELISPOT)Info
PM-HPV35-E6PepMix™ HPV35 (E6) Pool of 35 peptides derived from a peptide scan through Protein E6 of Human papillomavirus type 35 for T cell assays (e.g. ELISPOT)Info
PM-HPV35-E7PepMix™ HPV35 (E7) Pool of 22 peptides derived from a peptide scan through Protein E7 of Human papillomavirus type 35 for T cell assays (e.g. ELISPOT)Info
PM-HPV45-E6PepMix™ HPV45 (E6) Pool of 37 peptides derived from a peptide scan through Protein E6 of Human papillomavirus type 45 for T cell assays (e.g. ELISPOT)Info
PM-HPV45-E7PepMix™ HPV45 (E7) Pool of 24 peptides derived from a peptide scan through Protein E7 of Human papillomavirus type 45 for T cell assays (e.g. ELISPOT)Info
PM-HPV58-E6PepMix™ HPV58 (E6) Pool of 35 peptides derived from a peptide scan through Protein E6 of Human papillomavirus type 58 for T cell assays (e.g. ELISPOT)Info
PM-HPV58-E7PepMix™ HPV58 (E7) Pool of 22 peptides derived from a peptide scan through Protein E7 of Human papillomavirus type 58 for T cell assays (e.g. ELISPOT)Info
PM-HRSVA-MSGPepMix™ HRSVA (Major Surface Glycoprotein G) Pool of 72 overlapping peptides from Major surface glycoprotein G (mG) of Human respiratory syncytial virus (RSV) for T cell assaysInfo
PM-HRSVB-FGF0PepMix™ HRSVB (Fusion Glycoprotein F0) Pool of 141 peptides derived from a peptide scan through Fusion glycoprotein F0 of Human respiratory syncytial virus for T cell assays (e.g. ELISPOT)Info
PM-HRSVB-NCPNPepMix™ HRSVB (Nucleocapsid Protein N) Pool of 95 peptides derived from a peptide scan through Nucleoprotein N of Human respiratory syncytial virus for T cell assays (e.g. ELISPOT)Info
PM-HSV2-E3PepMix™ HSV2 (E3 ICP0) Pool of 204 peptides derived from a peptide scan through E3 ubiquitin-protein ligase ICP0 of Human herpesvirus 2 for T cell assays (e.g. ELISPOT)Info
PM-HSV2-gDPepMix™ HSV2 (gD) Pool of 96 peptides derived from a peptide scan through Envelope glycoprotein D of Human herpesvirus 2 for T cell assays (e.g. ELISPOT)Info
PM-HSV2-R1PepMix™ HSV2 (R1) Pool of 283 peptides derived from a peptide scan through Ribonucleoside-diphosphate reductase large subunit of Human herpesvirus 2 for T cell assays (e.g. ELISPOT)Info
PM-HSV2-VP22PepMix™ HSV2 (VP22) Pool of 73 peptides derived from a peptide scan (15mers with 11 aa overlap) through Tegument protein VP22 (UniProt ID: P89468) of Human herpesvirus 2 (strain HG52)Info
PM-Kir4.1PepMix™ Human (Kir4.1) Pool of 92 peptides derived from a peptide scan (15mers with 11 aa overlap) through Kir4.1 (ATP-sensitive inward rectifier potassium channel 10, UniProt ID: P78508) of Homo sapiens.Info
PM-INFA-HABrisPepMix™ Influenza A (HA /Brisbane (H1N1)) Pool of 139 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/Brisbane/59/2007(H1N1)) for T cell assaysInfo
PM-INFA-HACalPepMix™ Influenza A (HA /California (H1N1)) Pool of 139 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/California/07/2009(H1N1)) for T cell assaysInfo
PM-INFA-HAIndoPepMix™ Influenza A (HA/Indonesia (H5N1)) Pool of 140 peptides derived from a peptide scan (15mers with 11 aa overlap) through Hemagglutinin (UniProt ID: A1BK62) of Influenza A virus (Indonesia (H5N1))Info
PM-INFA_MP1PepMix™ Influenza A (MP1 (H3N2)) Pool of 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (strain A/Aichi/2/1968 H3N2) for T cell assaysInfo
PM-INFA-MP1-H1N1PepMix™ Influenza A (MP1 /California (H1N1)) Pool of 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (A/California/08/2009(H1N1)) for T cell assaysInfo
PM-INFA-MP1H2N2PepMix™ Influenza A (MP1/AnnArbor (H2N2) Pool of 61 overlapping peptides from Matrix protein 1 (MP1) of Influenza A (H2N2) for T cell assays (e.g. ELISPOT)Info
PM-INFA_MP2PepMix™ Influenza A (MP2 /California (H1N1)) Pool of 22 peptides derived from a peptide scan through Matrix protein 2 of Influenza A virus (A/California/08/2009(H1N1)) for T cell assaysInfo
PM-INFA-MP2H2N2PepMix™ Influenza A (MP2/AnnArbor (H2N2) Pool of 22 overlapping peptides from Matrix protein 2 (MP2) of Influenza A (H2N2) for T cell assays (e.g. ELISPOT)Info
PM-INFA-NATurPepMix™ Influenza A (Neuraminidase/Turkey/1/2005(H5N1)) Pool of 110 peptides derived from a peptide scan through Neuraminidase of Influenza A for T cell assays (antigen specific T-cell stimulation)Info
PM-INFA_NAPepMix™ Influenza A (Neuroamidase /California (H1N1) Pool of 115 peptides derived from a peptide scan through Neuraminidase of Influenza A virus (A/California/08/2009(H1N1)) for T cell assaysInfo
PM-INFA_NPPepMix™ Influenza A (NP (H3N2)) Pool of 122 peptides derived from a peptide scan through Nucleocapsid protein of Influenza A virus (strain A/Kitakyushu/159/1993 H3N2) for T cell assaysInfo
PM-INFA-NPH2N2PepMix™ Influenza A (NP/AnnArbor (H2N2) Pool of 122 overlapping peptides from Nucleoprotein (NP) of Influenza A (H2N2) for T cell assays (e.g. ELISPOT)Info
PM-LISMO-LLOPepMix™ L.monocytogenes (LLO) Pool of 130 peptides derived from a peptide scan through Listeriolysin O of Listeria monocytogenes serovar 1/2a for T cell assays (e.g. ELISPOT)Info
PM-MYCTU-AG85BPepMix™ M. tuberculosis (Ag85B) Pool of 79 peptides derived from a peptide scan through Diacylglycerol acyltransferase/mycolyltransferase Ag85B of Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) for T cell assays (e.g. ELISPOT)Info
PM-MYCTU-CFP10PepMix™ M. tuberculosis (CFP-10) Pool of 23 peptides derived from a peptide scan through ESAT-6-like protein EsxB of Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) for T cell assays (e.g. ELISPOT)Info
PM-MYCTU-ESAT6PepMix™ M. tuberculosis (ESAT-6) Pool of 21 peptides derived from a peptide scan through 6 kDa early secretory antigenic target of Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) for T cell assays (e.g. ELISPOT)Info
PM-MYCTU-TB104PepMix™ M. tuberculosis (TB10.4) Pool of 22 peptides derived from a peptide scan through ESAT-6-like protein EsxH of Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) for T cell assays (e.g. ELISPOT)Info
PM-RLCV-BZLF1PepMix™ RLCV (BZLF1) Pool of 59 peptides derived from a peptide scan through BZLF1 of Macacine herpesvirus 4 (Rhesus lymphocryptovirus) for T cell assays (e.g. ELISPOT)Info
PM-RLCV-EBNA1PepMix™ RLCV (EBNA1) Pool of 125 peptides derived from a peptide scan through EBNA-1 of Macacine herpesvirus 4 (Rhesus lymphocryptovirus) for T cell assays (e.g. ELISPOT)Info
PM-MVA-105LPepMix™ VACV (Cell surface-binding protein, MVA105L) Pool of 74 peptides derived from a peptide scan through Cell surface-binding protein (MVA105L) of Vaccinia virus (strain Ankara) for T cell assaysInfo
PM-MVA-018LPepMix™ VACV (Host range protein 2, MVA018L) Pool of 35 peptides derived from a peptide scan through Host range protein 2 (MVA018L) of Vaccinia virus (strain Ankara) for T cell assayInfo
PM-VACV-L3LPepMix™ VACV (L3L) Pool of 85 peptides derived from a peptide scan through Protein L3 of Vaccinia Virus (strain WR) for T cell assays (e.g. ELISPOT)Info
PM-MVA-121LPepMix™ VACV (Major core protein P4a, MVA 121L)_2 Subpools Pool of 223 peptides (delivered in two subpools of 112 + 111 peptides) derived from a peptide scan through Major core protein P4a of Vaccinia virusInfo
PM-MVA-074RPepMix™ VACV (MVA 074R) Pool of 106 peptides derived from a peptide scan through Putative 49.8k protein (MVA 074R) of Vaccinia virus (strain Ankara) for T cell assaysInfo
PM-MVA-093LPepMix™ VACV (MVA093L) Pool of 79 peptides derived from a peptide scan through IMV heparin binding surface protein (MVA093L) of Vaccinia virus (strain Ankara) for T cell assaysInfo
PM-MVA-189RPepMix™ VACV (MVA189R) Pool of 45 peptides derived from a peptide scan through Putative 21.7k protein (MVA189R) of Vaccinia virus (strain Ankara) for T cell assaysInfo
PM-VZV-gEPepMix™ VZV (gE) Pool of 153 peptides derived from a peptide scan (15mers with 11 aa overlap) through Envelope glycoprotein E (UniProt ID: P09259) of Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)Info
PM-VZV-IE62PepMix™ VZV (IE62)_2 subpools Pool of 325 peptides (delivered in two subpools of 163 + 162 peptides) derived from a peptide scan through Transcriptional transactivator IE62 of Varicella-zoster virus (strain Oka vaccine)Info
PM-VZV-IE63PepMix™ VZV (IE63) Pool of 67 peptides derived from a peptide scan through Transcriptional transactivator IE63 of Varicella-zoster virus (strain Oka vaccine) for T cell assaysInfo
PM-YF-NS4BPepMix™ Yellow fever (NS4B) Pool of 60 peptides derived from a peptide scan through Yellow fever Non-structural protein 4B of Yellow fever virus for T cell assaysInfo
PM-ZEBOV-GPKikPepMix™ Zaire Ebola (GP/Kikwit-95) Pool of 176 peptides derived from a peptide scan through Envelope glyocprotein of Zaire Ebola virus for T cell assays (antigen specific T-cell stimulation)Info
PM-ZEBOV-GPMayPepMix™ Zaire Ebola (GP/Mayinga-76) Pool of 176 peptides derived from a peptide scan through Envelope glyocprotein of Zaire Ebola virus for T cell assays (antigen specific T-cell stimulation)Info
PM-ZEBOV-VSGPPepMix™ Zaire Ebola (Virion Spike Glycoprotein) Pool of 167 peptides derived from a peptide scan through Virion Spike Glycoprotein of Zaire ebolavirus for T cell assays (e.g. ELISPOT)Info
PM-ZIKV-CPepMix™ ZIKV (C) Ultra (Zika virus) Pool of 48 15meric peptides derived from Capsid protein C of Zika Virus (ZIKV) designed to cover the full sequence diversity of Zika Virus, used for T cell assays (e.g. ELISPOT)Info
PM-ZIKV-EPepMix™ ZIKV (E) Ultra (Zika virus) Pool of 146 15meric peptides derived from Envelope protein E of Zika Virus (ZIKV) designed to cover the full sequence diversity of Zika Virus, used for T cell assays (e.g. ELISPOT)Info
PM-ZIKV-MPepMix™ ZIKV (M) Ultra (Zika virus) Pool of 49 15meric peptides derived from Membrane glycoprotein M of Zika Virus (ZIKV) designed to cover the full sequence diversity of Zika Virus, used for T cell assays (e.g. ELISPOT)Info
PM-ZIKV-NS1PepMix™ ZIKV (NS1) Ultra (Zika virus) Pool of 166 15meric peptides derived from Nonstructural protein NS1 of Zika Virus (ZIKV) designed to cover the full sequence diversity of Zika Virus, used for T cell assays (e.g. ELISPOT)Info

Peptide Pools for Tumor Associated Antigens

PM-WRKPepMix™ Arabidopsis (WRKY47) Pool of 120 peptides derived from a peptide scan through Probable WRKY transcription factor 47 of Arabidopsis thaliana (Mouse-ear cress)Info
PM-AFPPepMix™ Human (AFP) Pool of 150 peptides derived from a peptide scan through Alpha-fetoprotein of Homo sapiens for T cell assays (e.g. ELISPOT)Info
PM-BRACPepMix™ Human (Brachyury) Pool of 106 peptides derived from a peptide scan through Brachyury protein of Homo sapiens for T cell assays (e.g. ELISPOT)Info
PM-CEAPepMix™ Human (CEA) Pool of 173 peptides derived from a peptide scan through Carcinoembryonic antigen-related cell adhesion molecule 5 of Homo sapiens (Human)Info
PM-CLD6PepMix™ Human (Claudin-6) Pool of 53 peptides derived from a peptide scan through Claudin-6 of Homo sapiens (Human) for T cell assays (antigen specific T-cell stimulation)Info
PM-CYC_B1PepMix™ Human (Cyclin-B1) Pool of 106 peptides derived from a peptide scan through G2/mitotic-specific cyclin-B1 of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PM-ERB_ECDPepMix™ Human (ErbB2_ECD) Pool of 161 peptides derived from a peptide scan through Receptor tyrosine-protein kinase erbB-2 (extracellular domain) of Homo sapiensInfo
PM-ERB_ICDPepMix™ Human (ErbB2_ICD) Pool of 146 peptides derived from a peptide scan through Receptor tyrosine-protein kinase erbB-2 (intracellular domain) of Homo sapiens (Human)Info
PM-H1.2PepMix™ Human (Histone H1.2) Pool of 51 peptides derived from a peptide scan through Histone H1.2 of Homo sapiens (Human) for T cell assays (antigen specific T-cell stimulation)Info
PM-H4PepMix™ Human (Histone H4) Pool of 23 peptides derived from a peptide scan through Histone H4 of Homo sapiens (Human) for T cell assays (e.g. antigen specific T-cell stimulation)Info
PM-HMHA1PepMix™ Human (HMHA1) Pool of 282 peptides derived from a peptide scan through Minor histocompatibility protein HA-1 of Homo sapiens for T cell assays (e.g. ELISPOT)Info
PM-hTERTPepMix™ Human (hTERT) Pool of 128 peptides derived from a peptide scan through Telomerase transcriptase of Homo sapiens for T cell assays (e.g. ELISPOT)Info
PM-MAGEA1PepMix™ Human (MAGEA1) Pool of 75 peptides derived from a peptide scan through Melanoma-associated antigen 1 (MAGEA1) of Homo sapiens (Human) for T cell assaysInfo
PM-MAGEA3PepMix™ Human (MAGEA3) Pool of 76 peptides derived from a peptide scan through Melanoma-associated antigen 3 (MAGEA3) of Homo sapiens (Human) for T cell assaysInfo
PM-MAGEA4PepMix™ Human (MAGEA4) Pool of 77 peptides derived from a peptide scan through Melanoma-associated antigen 4 (MAGEA4) of Homo sapiens (Human) for T cell assaysInfo
PM-MAGEC1PepMix™ Human (MAGEC1) Pool of 283 peptides derived from a peptide scan through MAGEC1 of Homo sapiens for T cell assays (e.g. ELISPOT)Info
PM-MamAPepMix™ Human (Mammaglobin A) Pool of 21 peptides derived from a peptide scan through Mammaglobin-A of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PM-MelAPepMix™ Human (Melan-A/MART-1) Pool of 27 peptides derived from a peptide scan through Melanoma antigen recognized by T-cells 1 (MART-1) of Homo sapiens for T cell assaysInfo
PM-GP100PepMix™ Human (Melanocyte protein Pmel 17 gp100) Pool of 163 peptides derived from a peptide scan through Melanocyte protein Pmel 17 (gp100) of Homo sapiens (Human) for T cell assaysInfo
PM-STEAP1PepMix™ Human (Metalloreductase STEAP1) Pool of 82 peptides derived from a peptide scan through Metalloreductase STEAP1 of Homo sapiens (Human) for T cell assaysInfo
PM-MUC1PepMix™ Human (Mucin-1) Pool of 115 peptides derived from a peptide scan through Mucin-1 of Homo sapiens (Human) for T cell assaysInfo
PM-cMycPepMix™ Human (Myc protein) Pool of 107 peptides derived from a peptide scan through Myc proto-oncogene protein of Homo sapiens (Human) for T cell assaysInfo
PM-NYEPepMix™ Human (NY-ESO-1) Pool of 43 peptides derived from a peptide scan through Cancer/testis antigen 1 (NY-ESO-1) of Homo sapiens for T cell assays (e.g. ELISPOT)Info
PM-p53PepMix™ Human (P53) Pool of 96 peptides derived from a peptide scan through Cellular tumor antigen p53 of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PM-PAPPepMix™ Human (PAP) Pool of 94 overlapping peptides from Prostatic acid phosphatase (PAP) of Homo sapiens (human) for T cell assays (e.g. ELISPOT)Info
PM-OIP4PepMix™ Human (Prame/OIP4) Pool of 125 peptides derived from a peptide scan through Melanoma antigen preferentially expressed in tumors (PRAME/OIP4) of Homo sapiens (Human)Info
PM-PSAPepMix™ Human (Prostate-specific antigen PSA) Pool of 63 peptides derived from a peptide scan through Prostate-specific antigen of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PM-PSMAPepMix™ Human (PSMA) Pool of 185 peptides derived from a peptide scan (15mers with 11 aa overlap) through Glutamate carboxypeptidase 2 (Prostate-specific membrane antigen, Folate hydrolase 1, NAALADase I, UniProt ID: Q04609) of Homo sapiensInfo
PM-SOX-2PepMix™ Human (SOX-2) Pool of 77 peptides derived from a peptide scan through Transcription factor SOX-2 of Homo sapiens (Human) for T cell assaysInfo
PM-SSX2PepMix™ Human (SSX2) Pool of 45 peptides derived from a peptide scan through Protein SSX2 of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PM-MMP11PepMix™ Human (Stromelysin-3, MMP11) Pool of 121 peptides derived from a peptide scan through Stromelysin-3 (MMP11) of Homo sapiens (Human) for T cell assaysInfo
PM-SurvivinPepMix™ Human (Survivin) Pool of 33 peptides derived from a peptide scan through Baculoviral IAP repeat-containing protein 5 (Survivin) of Human for T cell assaysInfo
PM-SYCP1PepMix™ Human (SYCP1) Pool of 242 peptides derived from a peptide scan through Synaptonemal complex protein 1 of Homo sapiens for T cell assays (e.g. ELISPOT)Info
PM-TARPPepMix™ Human (TCRgamma alternate reading frame protein TARP) Pool of 12 peptides derived from a peptide scan through TCRgamma alternate reading frame protein of Homo sapiens (Human) for T cell assaysInfo
PM-TERTPepMix™ Human (TERT) Pool of 281 peptides derived from a peptide scan (15mers with 11 aa overlap) through Telomerase reverse transcriptase (UniProt ID: O14746) of Homo sapiens Info
PM-TyrPepMix™ Human (Tyrosinase) Pool of 130 peptides derived from a peptide scan through Tyrosinase of Homo sapiens (Human) for T cell assays (antigen specific T-cell stimulation)Info
PM-TRP-2PepMix™ Human (Tyrosinase-related protein 2) Pool of 127 peptides derived from a peptide scan through L-dopachrome tautomerase (Tyrosinase-related protein 2) of Homo sapiens (Human)Info
PM-WT1PepMix™ Human (WT1/WT33) Pool of 110 peptides derived from a peptide scan through Wilms tumor protein (WT33) of Homo sapiens (Human) for T cell assays (e.g. ELISPOT)Info
PM-MM-CLD6PepMix™ Mouse (Claudin-6) Pool of 52 peptides derived from a peptide scan through Claudin-6 of Mus musculus for T cell assays (e.g. ELISPOT)Info
PM-MM-SurvivinPepMix™ Mouse (Survivin) Pool of 33 peptides derived from a peptide scan through Baculoviral IAP repeat-containing protein 5 of Mus musculus for T cell assays (e.g. ELISPOT)Info
PM-MM-TyrPepMix™ Mouse (Tyrosinase) Pool of 117 peptides derived from a peptide scan through Tyrosinase of Mus musculus (Mouse) for T cell assays (antigen specific T-cell stimulation)Info
PM-MM-WT1PepMix™ Mouse (WT1) Pool of 110 peptides derived from a peptide scan through Wilms tumor protein homolog of Mus musculus (Mouse) for T cell assays (e.g. ELISPOT)Info

Individual ELISPOT Peptides

We are happy to provide the individual peptides to validate results or for follow-up experiments.  Our custom peptide synthesis is quality controlled according to ISO 9001 standards to ensure batch-to-batch reproducibility!

Applications for PepMix™ Peptide Pools

Benefits of PepMix™ Peptide Pools

Overlapping peptide pools are preferable to whole proteins for antigen-specific T-cell stimulation because:

  • Equivalent or better stimulation of CD4 and CD8 cells
  • More reliable as control than phytohemagglutinin (PHA) and concanavalin A (ConA)
  • Improved responses in stored blood and PBM cells
  • High-batch-to-batch reproducibility
  • Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation
  • Prolonged shelf stability when stored freeze dried

    Testimonials for PepMix™ Peptide Pools

    "To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D .  Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids.  Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
    Dr Laura J Pallett, Infection and Immunity, University College London, UK.

    "The main focus of my research group at the Charité in Berlin is the development of novel immunotherapeutic approaches against cancer and infectious diseases. For reliable monitoring of tumor and virus specific T-cell responses, we have a permanent need for peptide synthesis and peptide pools that are produced in a regulated environment for application in a clinical environment. JPT has been a long term and dedicated partner in this regard, which continuously works on improving it's peptide based services."
    Prof. Dr. Carmen Scheibenbogen, Charité Berlin, Germany

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    Quality Assurance

    All production is performed according to ISO 9001:2015 standards

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