T cells recognize specific MHC-peptide complex displayed on the surface of antigen-presenting cells. This specific interaction is used to detect and isolate antigen-specific T cells using MHC multimers carrying MHC-peptide complexes that bind to T cell receptors on T cells. Fluorescent-labeled MHC multimers are used to detect, quantify and isolate antigen-specific T cells in fluid samples (e.g. blood, cultured cell lines, CSF, lymph, synovial fluid) by flow cytometry, and for in situ detection (e.g. in solid tumors) by immunohistochemistry (IHC).
Find the related Antigen Peptide for each MHC Multimer or order your antigen by custom peptide synthesis!
- Stabilizing dextran polymer backbone
- Fluorochrome molecules
- More MHC-peptide complexes than conventional MHC multimers (such as tetramers)
- Stronger staining intensity
- Increased resolution and signal-to-noise ratio
MHC Dextramers detect the presence of antigen-specific T cells whereas interferon-gamma-releasing assays such as ELISpot detect the functionality of the antigen-specific T cells. Thus, a combination of the MHC Dextramer assay and an interferon-gamma-releasing assay, using PepMix™Peptide Pools as specific stimulating antigens, is a strong tool for reliable and robust immune monitoring!
Individual MHC I Dextramers®
More than 400 different MHC Dextramers® are available in our online shop
Selection of available Alleles:
|Human alleles||Mouse alleles||Monkey alleles|
MHC I Dextramer® Collections
Individual MHC II Dextramers®
Please visit our online shop for a list of MHCII Dextramers® and pricing.
Custom MHC I Dextramers®
Fast delivery of custom MHC Dextramers® upon request.
- Detection, enumeration and isolation of antigen-specific T cells in fluid cell samples (PBMC, blood) by flow cytometry
- In vitro staining of tissue sections to visualize antigen-specific T cells
- Vaccine and immunotherapeutic development
- Clinical trials
- Immune monitoring of e.g. transplantation patients and HIV/AIDS patients