Detection of Antigen-Specific T-cells with MHC Multimers
T cells recognize specific MHC-peptide complexes displayed on the surface of antigen-presenting cells. This specific interaction is used to detect and isolate antigen-specific T cells using MHC multimers carrying MHC-peptide complexes that bind to T cell receptors on T cells. Fluorescent-labeled MHC multimers are used to detect, quantify and isolate antigen-specific T cells in fluid samples (e.g. blood, cultured cell lines, CSF, lymph, synovial fluid) by flow cytometry, and for in situ detection (e.g. in solid tumors) by immunohistochemistry (IHC).
Find the related Antigen Peptide for each MHC Multimer!
- Stabilizing dextran polymer backbone
- Fluorochrome molecules
- More MHC-peptide complexes than conventional MHC multimers (such as tetramers)
- Stronger staining intensity
- Increased resolution and signal-to-noise ratio
MHC Dextramers detect the presence of antigen-specific T cells whereas interferon-gamma-releasing assays such as ELISPOT detect the functionality of the antigen-specific T cells. Thus, a combination of the MHC Dextramer assay and an interferon-gamma-releasing assay, using PepMix™Peptide Pools as specific stimulating antigens, is a strong tool for reliable and robust immune monitoring!
Individual MHC I Dextramers®
More than 400 different MHC Dextramers® are available in our online shop
Selection of available Alleles:
|Human alleles||Mouse alleles||Monkey alleles|
MHC I Dextramer® Collections
Custom MHC I Dextramers®
Fast delivery of custom MHC Dextramers® upon request.
Applications for MHC Multimers (Dextramers®)
- Detection, enumeration and isolation of antigen-specific T cells in fluid cell samples (PBMC, blood) by flow cytometry
- In vitro staining of tissue sections to visualize antigen-specific T cells
- Vaccine and immunotherapeutic development
- Clinical trials
- Immune monitoring of e.g. transplantation patients and HIV/AIDS patients
Selected References for MHC Dextramers®
"Comparison of Peptide-major Histocompability Complex Tetramers and Dextramers for the Identification of Antigen-specific T Cells"
Dolton et al., Clin Exp Immunol. (2014)- PMID: 24673376
"Combination Immunotherapy after ASCT for Multiple Myeloma Using MAGE-A3/Poly-ICLC Immunizations Followed by Adoptive Transfer of Vaccine-Primed and Costimulated Autologous T Cells"
Rapoport et al., Clin Cancer Res (2014) - PMID: 24520093
"Molecular Imprint of Exposure to Naturally Occurring Genetic Variants of Human Cytomegalovirus on the T cell Repertoire"
Smith et al., Sci Rep (2014) - PMID: 24509977
"The Transcription Factor Interferon Regulatory Factor 4 is Required for the Generation of Protective Effector CD8+ T cells"
Raczkowskia et al., PNAS (2013) - PMID: 23980171
"Probing the effector and suppressive functions of human T cell subsets using antigen-specific engineered T cell receptors"
Wan et al., PLoS One (2013) Full text
"Human Natural Killer Cells Prevent Infectious Mononucleosis Features by Targeting Lytic Epstein-Barr Virus Infection"
Chijioke et al., Cell Rep (2013) - PMID: 24360958
Read More References with MHC Multimers
PepMix™ Peptide Pools
Antigen spanning peptide pools for T cell stimulation with virus or tumor associated antigens (e.g. HIV, HPV, melanoma) during T cell assays such as ELISPOT.
PepTrack™ Peptide Libraries
Advanced peptide library generation for the specific requirements of T-cell assays.
Performance Control of T-cell Assays
Check the innovative, easy method to produce cellular reference samples for antigen-specific T-cell assays (TERS).
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