MHC Multimers

Detection of Antigen-Specific T-cells with MHC Multimers

T cells recognize specific MHC-peptide complexes displayed on the surface of antigen-presenting cells. This specific interaction is used to detect and isolate antigen-specific T cells using MHC multimers carrying MHC-peptide complexes that bind to T cell receptors on T cells. Fluorescent-labeled MHC multimers are used to detect, quantify and isolate antigen-specific T cells in fluid samples (e.g. blood, cultured cell lines, CSF, lymph, synovial fluid) by flow cytometry, and for in situ detection (e.g. in solid tumors) by immunohistochemistry (IHC).
Find the related Antigen Peptide for each MHC Multimer!

MHC Dextramers®

  • Stabilizing dextran polymer backbone
  • Fluorochrome molecules
  • More MHC-peptide complexes than conventional MHC multimers (such as tetramers)
  • Stronger staining intensity
  • Increased resolution and signal-to-noise ratio

MHC Dextramers detect the presence of antigen-specific T cells whereas interferon-gamma-releasing assays such as ELISPOT detect the functionality of the antigen-specific T cells. Thus, a combination of the MHC Dextramer assay and an interferon-gamma-releasing assay, using PepMix™Peptide Pools as specific stimulating antigens, is a strong tool for reliable and robust immune monitoring!

Individual MHC I Dextramers®

More than 400 different MHC Dextramers® are available in our online shop

Selection of available Alleles:

Human alleles Mouse alleles Monkey alleles
HLA-A*0101 HLA-B*0702 H-2 Db Mamu-A*01
HLA-A*0201 HLA-B*0801 H-2 Dd Mamu-A*04
HLA-A*0301 HLA-B*2705 H-2 Dk Mamu-A*08
HLA-A*1101 HLA-B*3501 H-2 Kb Mamu-B*17
HLA-A*2301 HLA-B*4201 H-2 Kd
HLA-A*2402 HLA-B*4403 H-2 Kk
HLA-A*2902 HLA-B*5101 H-2 Ld
HLA-A*6801 HLA-B*5701
HLA-B*5703
HLA-CW*0304

MHC I Dextramer® Collections

RX02Cancer-Testis Antigens Dextramer Collection 1 Multimer collection of 3 carefully selected cancer-testis specific antigen Dextramers and a control. Two aliquots / antigen with different fluorescent labels (PE and APC) are delivered Info
CMV-PACK-APCCMV Dextramer Package 1-APC Multimer collection of 8 selected CMV antigen Dextramers and one control. The set comes with APC labelInfo
CMV-PACK-FITCCMV Dextramer Package 1-FITC Multimer collection of 8 selected CMV antigen Dextramers and one control. The set comes with FITC labelInfo
CMV-PACK-PECMV Dextramer Package 1-PE Multimer collection of 8 selected CMV antigen Dextramers and one control. The set comes with PE labelInfo
RX05-APCEBV Dextramer Package 1-APC Multimer collection of 4 selected EBV antigen Dextramers and one control. The set comes with APC labelInfo
RX05-FITCEBV Dextramer Package 1-FITC Multimer collection of 4 selected EBV antigen Dextramers and one control. The set comes with FITC labelInfo
RX05-PEEBV Dextramer Package 1-PE Multimer collection of 4 selected EBV antigen Dextramers and one control. The set comes with PE labelInfo
RX01Melanoma Dextramer Collection 1 Multimer collection of 6 carefully selected melanoma specific antigen Dextramers and one control. Two aliquots / antigen with different fluorescent labels (PE and APC) are delivered Info
RX03-PEVirus Dextramer Collection 1-PE Multimer collection of 4 selected virus (CMV, EBV, BKV & Influenza) antigen Dextramers and one control. The set comes with PE labelInfo
RX03-APCVirus Dextramer Collection 1-APC Multimer collection of 4 selected virus (CMV, EBV, BKV & Influenza) antigen Dextramers and one control. The set comes with APC labelInfo
RX03-FITCVirus Dextramer Collection 1-FITC Multimer collection of 4 selected virus (CMV, EBV, BKV & Influenza) antigen Dextramers and one control. The set comes with FITC labelInfo

Custom MHC I Dextramers®

Fast delivery of custom MHC Dextramers® upon request.

Applications for MHC Multimers (Dextramers®)

  • Detection, enumeration and isolation of antigen-specific T cells in fluid cell samples (PBMC, blood) by flow cytometry
  • In vitro staining of tissue sections to visualize antigen-specific T cells
  • Vaccine and immunotherapeutic development
  • Clinical trials
  • Immune monitoring of e.g. transplantation patients and HIV/AIDS patients

Benefits of MHC Dextramers®

  • More sensitive  than conventional MHC multimers (important for low-affinity interactions commonly encountered in cancer & autoimmune disease)
  • Very stable
  • Order the related Antigen Peptide together with your MHC Dextramer
  • Customized Dextramers® available upon request

Selected References for MHC Dextramers®

"Comparison of Peptide-major Histocompability Complex Tetramers and Dextramers for the Identification of Antigen-specific T Cells"
Dolton et al., Clin Exp Immunol. (2014)- PMID: 24673376
"Combination Immunotherapy after ASCT for Multiple Myeloma Using MAGE-A3/Poly-ICLC Immunizations Followed by Adoptive Transfer of Vaccine-Primed and Costimulated Autologous T Cells"
Rapoport et al., Clin Cancer Res (2014) - PMID: 24520093
"Molecular Imprint of Exposure to Naturally Occurring Genetic Variants of Human Cytomegalovirus on the T cell Repertoire"
Smith et al., Sci Rep (2014) - PMID: 24509977
"The Transcription Factor Interferon Regulatory Factor 4 is Required for the Generation of Protective Effector CD8+ T cells"
Raczkowskia et al., PNAS (2013) - PMID: 23980171
"Probing the effector and suppressive functions of human T cell subsets using antigen-specific engineered T cell receptors"

Wan et al., PLoS One (2013) Full text
"Human Natural Killer Cells Prevent Infectious Mononucleosis Features by Targeting Lytic Epstein-Barr Virus Infection"

Chijioke et al., Cell Rep (2013) - PMID: 24360958

Read More References with MHC Multimers

Related Products

PepMix™ Peptide Pools
Antigen spanning peptide pools for T cell stimulation with virus or tumor associated antigens (e.g. HIV, HPV, melanoma) during T cell assays such as ELISPOT.

PepTrack™ Peptide Libraries

Advanced peptide library generation for the specific requirements of T-cell assays.

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