Fluorescent Peptides & Dye Labeled Peptides

Dye and fluorescent labeled peptides are commonly used for protein binding or localization studies.
These labels can be incorporated at many positions in the fluorescent peptide or labeled peptide. Usually, they are incorporated at the N-terminus or at Cys/Lys side chains. Examples of frequently used fluorescent dyes and fluorescence labels and common attachment options for fluorescent labeled peptides are listed below.

Fluorophore Short Name Full Name Excitation / Emission Wavelength
Abz Anthranilyl, 2-Aminobenzoyl 320 nm
420 nm
N-Me-Abz N-Methyl-anthranilyl, N-Methyl-2-Aminobenzoyl 340-360 nm
440-450 nm
FITC Fluoresceinisothiocyanate (5 or 6 isomer or mixture of isomers) 490 nm
520 nm
5-FAM 5-Carboxyfluorescein 492 nm
517 nm
6-FAM 6-Carboxyfluorescein 492 nm
517 nm
TAMRA Carboxytetramethyl rhodamine
(5 or 6 isomer or mixture of isomers)
543 nm
572 nm
Mca 7-Methoxycoumarinyl-4-acetyl 325 nm
392 nm
AMCA / Amc Aminomethylcoumarin Acetate 350 nm
450 nm
Dansyl 5-(Dimethylamino) naphthalene-1-sulfonyl 342 nm
562 nm
EDANS 5-[(2-Aminoethyl)amino] naphthalene-1-sulfonic acid 340 nm
490 nm
Atto e.g. Atto465, Atto488, Atto495, Atto550, Atto647 453 nm
508 nm
Cy3 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride 550 nm
570 nm
Cy5 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride 650 nm
670 nm
Alexa Fluor e.g. Alexa Fluor 647, Alexa488, Alexa532, Alexa546, Alexa594, Alexa633, Alexa647 650 nm
668 nm
Bodipy e.g. Bodipy® FL 505 nm
511 nm
Dylight e.g. DyLight 488, DyLight 550 493nm
518 nm
Trp Tryptophan 280 nm
360 nm
Lucifer Yellow (ethylene diamine) 6-Amino-2-(2-amino-ethyl)-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinoline-5,8-disulfonic acid 430 nm
520 nm

Fluorescence & Dye Labeled Peptides

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