Enzyme Substrate Peptide Microarrays

Enzyme Substrate Microarrays (Enzyme Substrate Peptide Microarrays) display unique sets of enzyme substrate peptides for kinase and phosphatase derived from human phosphorylation sites, or calculated by algorithms. Enzyme Substrate Peptide Microarrays are applicable for direct incubation with purified enzymes or other fluids.
JPT’s peptide microarrays on glass slides enable characterization of enzymes such as kinases and phosphatases as well as methyl, glycosyl, acetyl transferases. Peptides are immobilized on glass slides via a flexible linker. Chemoselective coupling generates microarrays displaying covalently attached peptides in multiple identical subarrays. Incubation can be performed manually or automated.

Please inquire for our tailored PepStar™ peptide microarrays!

Applications for Enzyme Substrate Microarrays

  • Enzyme substrate identification
  • Consensus sequence determination
  • Profiling of orphan enzymes

Application Notes:
"Unraveling Phosphorylation of the Splicing Factor SC35 by the Dual Signaling Kinase PKC-Θ in Human T-Cells "
by McCuaig et al.

Benefits of Enzyme Substrate Microarrays

  • Screen hundreds of potential peptide substrates in parallel
  • Identify key residues and consensus sequences
  • Take advantage of a reliable and reproducible screening tool
  • Obtain the individual kinase substrate peptides for validation of results

Testimonials for Enzyme Substrate Microarrays

"We have successfully applied JPT's peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures."
Dr. Marcelo P. Coba (The Wellcome Trust Sanger Institute, Cambridge, UK)

"With JPT´s peptide microarray platform, high- throughput kinase profiling using small amounts of cell lysate becomes feasible, providing access to the discovery and monitoring of novel biomarkers in Parkinson's Disease."
Jeremy Nichols, PhD (Parkinsons's Institute, Sunnyvale, CA, USA)

" JPT’s new extremely high-density histone peptide array is a dramatic leap forward for analysis of the histone code hypothesis. The complete sequence coverage across core histones and histone variants as well as the presence of common and rare histone post-translational modifications – alone and in combination – makes this an unparalleled tool for studying the “writers” and “readers” of the histone code. My laboratory has used these arrays to ask the sequence and PTM dependence of a histone writer enzyme and we have discovered an activity specifying code that will keep us busy for years to come."
David Shechter, PhD, Department of Biochemistry, Albert Einstein College of Medicine,  Yeshiva University, NY, USA 

More testimonials

Related products and services

Single Kinase Substrate Peptides
The substrates represent 13meric peptides derived from phosphorylation sites of human proteins.
Please select from the list of nearly 800 peptides currently available.
Enzyme Substrate Sets
Compilation of enzyme substrate peptides in ready-to-use microtiter plates.
Peptide Microarray Assay Service
Profiling service using high content PepStar peptide microarrays.

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