Acetylation of lysine ( resulting in acetyl lysine) residues in proteins and peptides is a post-translational modification (PTM) that plays a major role in regulating transcription and other metabolic processes. Acetyl lysine was originally discovered in histone proteins about 40 years ago, the extent of this modification in other cellular proteins, so-called non-histone targets, has been largely underestimated. Recent mass spectrometric investigations have identified more than 5,500 lysine acetylation sites in human proteins.
Acetylome - Acetyltransferase & Deascetylase Microarrays:
- High-density peptide microarrays
- 5599 peptides derived from reported human lysine acetylation sites on each microarrays
- 384 control peptides are added to each microarray
- 5599 acetylated peptides are presented on the Acetylome - Deacetylase Microarray
- The same 5599 peptides in unmodified form are on the Acetylome- Acetyltransferase Microarray
- Peptides are immobilized in triplicates onto glass slides
- Incubation possible with biological samples, such as purified antibodies or enzymes, cell lysates or patient sera
- We also offer an incubation and analysis service
- For individual acetylated peptides please refer to our custom peptide synthesis
Acetylome - Acetyltransferase & Deacetylase Microarrays
- Analysis of binding specificities of acetylation-specific protein domains
- Profiling of lysine-acetyltransferases and lysine-deacetylases
- Determination of specific substrates
- Identification of epigenetic targets involved in the human acetylome
"Exploring the Human Acetylome Using High-Density Peptide Microarrays"
by M. Schutkowski
- All human acetylation sites on one microarray
2 microarrays (acetylated vs. non-acetylated lysines)
- Peptides are free of truncated sequences
- Applicable to a variety of biological samples
- Low background on glass surface, read-out via fluorescence
- Screen thousands of potential peptide substrates economically
- Readout is performed by differential measurement, allowing comparative analysis (a control microarray is provided with each order)
- Kinase Peptide Microarrays: Peptide microarrays containing either annotated human phosphorylation site derived peptides or randomly generated peptide for identification of kinase substrates and profiling of kinase substrate specificities (also available with full incubation service).
- Phosphatase Peptide Microarrays: Compilation of phosphatase substrate peptides on ready-to-use PepStar peptide microarrays.