Contact

Absolute Quantified Peptides - SpikeTides™ TQL & SpikeTides™ TQL PLUS

Absolute quantified peptides are used as internal references (absolute quantified reference peptides) for protein quantitation in mass spectrometry based proteomics, e.g. for selected reaction monitoring (SRM), multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM). 

Our proprietary absolute peptide quantitation method overcomes the limitations of traditional methods such as limited accuracy and high costs:

  • JPT’s QTag is a small chemical tag attached to each peptide
  • Robust and reproducible quantitation
  • Peptide quantification via HPLC-UV or HPLC-MS and UV
  • QTag is released by trypsin and does not interfere with measurements
  • Very accurate method (variation CV= 5%)
  • Validated in numerous peer-reviewed papers
  • Lower cost than traditional peptide quantitation
  • Up-to-date quantification immediately before experiment for clinical applications (SpikeTides™_TQL PLUS)

Comparison of Absolute Quantified Peptides:

rUo spiketides™_TQL Clinical spiketides™_TQL plus
Quantification                     x                                  x
Quantification by end-user possible                                  x
Quantification method           UV / QTag                         UV / QTag
Purified                     x                                 x
Stable isotope-labeled*                     x                                 x
Amount            5x1 nmol                           5x10 nmol
Isotopical purity                >99%                                >99%
Incoming material inspection                      x                                 x
Vendor qualification                      x                                 x
Batch documentation & CoA                      x                                 x
Optional: certified vials                                 x
Optional: additional QC methods                                 x

*light versions are also available

Also have a look at our Reference Peptides for Targeted Proteomics -SpikeTides™ & SpikeMix™ without absolute quantitation.

Select your Absolute Quantified Peptides:

SpikeTides™_TQ - light quantified peptides

SpikeTides™_TQ Proteotypic Peptides

SpikeTides™_TQ are quantified, proteotypic peptides that have a C-terminal tag that can be cleaved by tryptic digestion.
Amounts: 5 x 1 nmol/target peptide
Purity: Purified
Application: Absolute protein quantification
Price: from 125 US$ / 108 € (depending on number of peptides ordered)
Validated pooling service available upon request!

SpikeTides TQ

SpikeTides™_TQL - isotopically labeled & quantified peptides

SpikeTides™_TQL Proteotypic Peptides

SpikeTides™_TQL are heavily labeled, quantified, proteotypic peptides that have a C-terminal tag that can be cleaved by tryptic digestion.
Amounts: 5 x 1 nmol/target peptide
Purity: Purified
Application: Absolute protein quantification
Price: from 198US$/184€
Validated pooling service available upon request!

Spike Tides TQL

SpikeTides™_TQL PLUS - for clinical proteomics

SpikeTides™_TQL PLUS are stable isotope-labeled, customized peptides that have a C-terminal QTag that can be cleaved by tryptic digestion. It allows you to quantify peptides immediately before your experiment.
Amounts: 5 x 10 nmol/target peptide
Purity: Purified
Application: Absolute protein quantification
Validated pooling service available upon request!

Maxi SpikeTides™ - options for large peptide amounts

Of course, all SpikeTides™ options can be adjusted to meet your requirements. Maxi SpikeTides™ are synthesized by standard resin-based custom peptide synthesis and very flexible. Choosing Maxi SpikeTides™ enables:

  • longer peptides
  • higher amounts per peptide
  • guaranteed purity for each peptide
  • no minimum peptide number
  • classical AAA quantification of peptides available
  • use of various PTMs (Post-Translational Modifications)
  • validated pooling service available

    Please inquire for your Maxi SpikeTides™ via email!

    Applications for Absolute Quantified Peptides

    Benefits for Absolute Quantified Peptides

    • Nondestructive quantification method
    • Re-quantification at endusers laboratory feasible
    • HPLC-purified peptides
    • Enables monitoring of peptide stability, precipitation and adhesion
    • Absolute and direct quantitation
    • SpikeTides™ TQL approach is robust and cost effective
    • Fully compatible with downstream analyses like MS and HPLC
    • Enables chemical barcoding of complex SpikeTides™ mixtures within the JPT-tag

    Testimonial for Absolute Quantified Peptides

    "My unit at the Banting and Best Department of Medical Research, University of Toronto Centre for Cellular and Biomolecular Research (CCBR) works on the systematic identification and quantification of proteins and protein complexes on a global level. In addition we have started to perform targeted proteomics to study protein regulation within pathways, e.g. signaling pathways during stem cell fate decision. For this we used JPT's various SpikeTides™ peptide products to build LC-MRM assays in a fast and cost efficient manner. More recently we also found new ways of using low cost SpikeTides™ as quantitative peptide standards in order to quantify protein targets more accurately. We are currently in the process of validating this technology for the accurate quantification of various predicted microRNA targets."
    Prof. Andrew Emili (Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Canada)

    > Read more Testimonials

    News Highlights

    --> Read more news

    Quality Assurance

    All production is performed according to ISO 9001:2015 standards

    Stay in touch and be the first to receive the latest news!