Fluorescent Peptides & Dye Labeled Peptides

Dye and fluorescent labeled peptides are commonly used for protein binding or localization studies.

These labels can be incorporated at many positions in the fluorescent peptide or labeled peptide. Usually, they are incorporated at the N-terminus or at Cys/Lys side chains. Examples of frequently used fluorescent dyes and fluorescence labels and common attachment options for fluorescent labeled peptides are listed below.

Fluorophore Short Name Full Name Excitation / Emission Wavelength
Abz Anthranilyl, 2-Aminobenzoyl

320 nm
420 nm

N-Me-Abz N-Methyl-anthranilyl, N-Methyl-2-Aminobenzoyl

340-360 nm
440-450 nm

FITC Fluoresceinisothiocyanate (5 or 6 isomer or mixture of isomers)

490 nm
520 nm

5-FAM 5-Carboxyfluorescein

492 nm
517 nm

6-FAM 6-Carboxyfluorescein

492 nm
517 nm

TAMRA Carboxytetramethyl rhodamine
(5 or 6 isomer or mixture of isomers)

543 nm
572 nm

Mca 7-Methoxycoumarinyl-4-acetyl

325 nm
392 nm

AMCA / Amc Aminomethylcoumarin Acetate

350 nm
450 nm

Dansyl 5-(Dimethylamino) naphthalene-1-sulfonyl 342 nm
562 nm
EDANS 5-[(2-Aminoethyl)amino] naphthalene-1-sulfonic acid

340 nm
490 nm

Atto e.g. Atto465, Atto488, Atto495, Atto550, Atto647

453 nm
508 nm

Cy3 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride

550 nm
570 nm

Cy5 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride

650 nm
670 nm

Alexa Fluor e.g. Alexa Fluor 647, Alexa488, Alexa532, Alexa546, Alexa594, Alexa633, Alexa647

650 nm
668 nm

Bodipy e.g. Bodipy® FL

505 nm
511 nm

Dylight e.g. DyLight 488, DyLight 550

518 nm

Trp Tryptophan

280 nm
360 nm

Lucifer Yellow (ethylene diamine) 6-Amino-2-(2-amino-ethyl)-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinoline-5,8-disulfonic acid

430 nm
520 nm

Fluorescence & Dye Labeled Peptides
fluorescence dye modifications

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