Peptide Synthesis
Peptide synthesis is the controlled production of defined amino-acid sequences using chemical synthesis technologies such as solid-phase peptide synthesis (SPPS). JPT combines multiple synthesis platforms and production scales to support research, screening, immunology, proteomics, diagnostics and drug-discovery workflows.
Our peptide synthesis capabilities range from micro-scale high-throughput production to milligram, gram and larger-scale projects, with options for purification, modification, labeling, pooling, formatting and analytical quality control.
peptide expertise
peptides synthesized per year
Global customers
Articles citing JPT's peptides
What Is Peptide Synthesis?
Peptide synthesis is the stepwise assembly of amino acids into a defined peptide sequence. In chemical peptide synthesis, the order of amino acids is controlled through repeated coupling and deprotection reactions. Protecting groups help direct each reaction to the intended functional group, while purification and analytical testing are used to verify the final product.
For many research peptides, solid-phase peptide synthesis is the core technology because it enables efficient sequential chain elongation on a resin-bound support. Other approaches, including high-throughput SPOT synthesis, ligation and fragment condensation, can be used when project scale, sequence complexity or library design requires a different strategy.
Peptide Synthesis Methods
Solid-Phase Peptide Synthesis (SPPS)
In SPPS, the peptide chain is assembled while attached to a solid support. Repeated deprotection, amino-acid coupling and washing cycles enable controlled chain elongation. JPT uses Fmoc SPPS for many peptide projects.
High-Throughput & SPOT Synthesis
Parallel synthesis technologies support projects that require many related sequences, including screening libraries, peptide arrays and other high-throughput applications where scale and format differ from conventional single-peptide production.
Ligation & Fragment Condensation
For selected long or challenging targets, peptide fragments can be prepared separately and joined in a subsequent step. The most appropriate route depends on sequence length, chemistry, required modifications and final project specifications.
Peptide Synthesis Across Scales and Formats
| Quantity | Purity | Modifications | Format | Optional Quantification | Quality Grade | Benefits | |
| Micro Scale Peptide Synthesis | 10 - 50µg | crude, crude with guarantee | modified aa, biotin, phosphorylation, stable-isotope labeled ... | tubes, pools, microtiter plates, microarrays, cellulose, ELISA | OD | research use | From only 15€/US$19.50 per peptide! >50,000 peptides/month |
|
Mid Scale Synthesis
| 1mg - 1g | crude to >95% | modified aa, biotin, phosphorylation, stable-isotope labeled, AQUA, cyclization… | tubes, pools, tube racks, ELISA | Q tag, AAA | research use, immunomonitoring, cell therapy development, clinical trial immune monitoring | Premium quality >5000 peptides/month |
| Large Scale Synthesis | > 1g | crude to >95% | modified aa, biotin, phosphorylation, stable-isotope labeled, cyclization, lipids, TFA removal… | tubes, pools | Q tag, AAA | research use, immunomonitoring, cell therapy development, clinical trial immune monitoring | Endless range of modifications |
Why Choose JPT?
- ISO 9001:2015 Certified: Our processes follow rigorous, audited quality standards for consistent and dependable peptide synthesis.
- Berlin-Based Manufacturing: All peptide synthesis is performed in our Berlin facilities, ensuring controlled, reliable production.
- Global Scientific Support: With teams in Berlin, the UK and the US, expert guidance is available for any peptide-related project.
- Two Decades of Expertise: Our long-standing experience positions us as true leaders in the peptide synthesis industry.
- Ethical and Sustainable Practices: We maintain strict environmental, health, and ethical standards across all production steps.
Explore JPT Peptide Synthesis Capabilities
Custom Peptide Synthesis
Peptide Libraries
Peptide Pools
Peptide Modifications
Bioconjugated Peptides
Peptide Arrays & Microarrays
Critical Raw Material
Peptide ELISA
Peptide Analysis
Find the Peptide Synthesis Service You Need Here
Testimonials
"The RV 144 HIV trial is considered as one of first successful HIV vaccine trials. The use of JPT's PepStarTM microarray technology has been very useful for the correlation of the clincial outcome with humoral immune responses. As have the cyclic peptides been from JPT to validate these findings!"
J. Currier, PhD, Walter Reed Army Institute, Rockville, Maryland, USA
"We utilize 15-mer PepMix™ peptide pools encoding for weak tumor associated antigens for immunomonitoring of cancer patients treated with recombinant therapeutic vaccines. Our experience with JPT has been outstanding in regard to product quality and communication with scientific and administrative customer service."
Benedetto Farsaci, MD, National Cancer Institute, NIH, Bethesda, MD, USA
"Our teams at the Vrije Universiteit Brussel deal with a wide variety of projects in different areas of applied biological sciences. For many of them we are in need of peptide related tools and services and we are collaborating with JPT for many years and are very satisfied with the relationship which led to several well received publications."
Prof. Dr. Joost Schymkowitz (Vrije Universiteit, Brussel, Belgium)
FAQs
What peptide lengths can JPT synthesize?
How can I design my peptide library or pool?
What methods does JPT use to synthesize peptides?
Can JPT synthesize peptides with unusual residues?
Yes. We routinely produce
peptides containing non-natural amino acids, non-commercially available
amino acids, and post-translational
modifications.
What is the difference between peptide content and peptide weight?
How to dissolve peptides?
Charged peptides dissolve best in aqueous buffers, while hydrophobic peptides may require initial solubilization in organic solvents such as DMSO or DMF. For more information visit our page on Dissolving Peptides.
How do we avoid cross-contamination?
We prevent cross-contamination using dedicated equipment, single-use consumables, strict cleaning protocols, and segregated workflows aligned with the required quality grade.
Does JPT consider sequence diversity?
Yes. With our ULTRA bioinformatics approach, we design peptide libraries that cover all relevant sequence variations across viral, bacterial, or cancer-related targets.
Can you synthesize modified peptides?
What does ISO 9001 certification mean?
What does average purity mean at JPT?
How do you reduce toxic by-products during synthesis?
To reduce toxic synthesis by-products, we purify all peptides with a guaranteed purity level regardless of whether the initial crude material already meets the target purity or not, which significantly reduces chemicals from the synthesis process. If your assay or application is very sensitive, we can substitute TFA with chloride or acetate.
How do you avoid de novo epitope formation?
Find the Right Peptide Synthesis Route for Your Project
Explore the relevant synthesis capability, or contact JPT's peptide experts if your sequence, scale or modification requires a project-specific discussion.