Stable Isotope Labeled Peptides: SpikeTides™
Stable Isotope Labeled (SIL) peptides are synthesized by substituting specific atoms in natural amino acids with heavy isotopes, such as replacing 12C with 13C, 14N with 15N, or 1H with 2H (deuterium). These peptide modifications result in isotope labeled peptides that retain the same physiochemical properties and chemical reactivity as their non-labeled counterparts while increasing their mol weight. This makes SIL peptides indispensable in a wide array of scientific applications, including mass spectrometry quantification, LC-MS/MS workflows, NMR studies, and pharmacokinetic analyses.
Stable Isotope Labeled Peptides for Biomarker Discovery, Bioanalyses, and Research
JPT Peptide Technologies has been a pioneer in stable isotope labeled peptide synthesis and offers multiple solutions, including absolutely quantified SIL peptides, supporting research in biomarker discovery, bioanalysis, peptidomics, quantitative proteomics, and structural biology.
SpikeTides™ products and services encompass custom synthesis of large peptide libraries at micro scale, ideal for biomarker discovery, as well as reference standards for the quantification of clinical analytes. Beside customized SIL peptides, JPT offers a unique range of off-the-shelf products including LCMS calibration standards, kits for PTM discovery, and heavy labeled peptide reference standards.
Custom SIL Peptides Synthesis Services
| Product | Specifications | Packagings | Quality grades | Modifications available |
|---|---|---|---|---|
|
Standard SpikeTides
(peptides quantified by standard methods) |
Micro scale Quantity: 3 or 10 µg Length: 5 to 20 aa Purity: crude (5 or 100% MS) Quantification: estimation |
Tubes, plates, pools Minimum to order: 24 peptides |
Research Use Only |
Isotope labeling: all standard amino acids possible with 13Cn/15N Modified AAs: CysCAM, PhosphoAAs, Met[O]… Additional modifications: further modifications such as cyclizations, lipidations… can be considered. Please reach out to customer service. |
|
Mid scale Quantity: 1 mg to +100 mg Length: 2 to +40 aa Purity: crude to >95% Quantification: powder weighing |
Tubes, plates, pools Aliquotation upon request No minimum to order |
Research Use Only |
||
|
AQUA SpikeTides
(absolutely quantified peptides) |
Micro scale Quantity: 10 to 150 nmol Length: 2 to +40 aa Purity: 70% to >95% Quantification: absolute quantification of SIL peptides by Q-tag or AAA |
Accurate aliquotation in 1 or 10 nmol in tubes, plates, pools No minimum to order |
Research Use Only | |
|
Mid scale Quantity: 1 mg to +100 mg Length: 2 to +40 aa Purity: >95% Quantification: absolute quantification by AAA |
Accurate aliquotation upon request from 1 nmol Tubes, plates, pools No minimum to order |
Research Use and IsoPlus: ideal for GLP studies or IVD materials (line clearance, stability testing plan…) |
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| Standard delivery times: 3–4 weeks for standard SpikeTides, 4–5 weeks for AQUA SpikeTides. Rush service available for urgent needs, please contact customer service. | ||||
Request a Quote for SIL Peptides
Production of Stable Isotope Labeled Peptides
JPT’s SpikeTides™ are produced chemically (no recombinant production). Chemical manufacturing allows fast versatile production of isotope labeled peptides, enabling incorporation of commercially available heavy labeled amino acids during synthesis.
The common labeling on the market is the double labeling of all carbon and nitrogen atoms from one amino acid. i.e. Arg 13C6 / 15N4. Most common doubly labeled amino acids are Arginine and Lysine. Less expensive ones are phenylalanine and leucine. Labeled amino acids are very expensive starting materials making SIL peptides typically more expensive than non-labeled peptides.
JPT developed micro scale SpikeTides solutions that allows to reduce consumption of material and achieve highly competitive pricing for customized SIL peptides while using best-in-class isotopic amino acids (enrichment >99%). JPT can introduce one or more heavy labeled amino acids in a sequence, depending on customer needs (c.f. delta-mass table). Other heavy amino acid labeling such as single labeling of carbons or hydrogens can also be available upon request.
Core Technologies for SIL peptides
- Micro scale platform SPOT: JPT’s proprietary peptide synthesis platform at the origin of JPT’s foundation! This platform allows ultra-high-throughput peptide synthesis production (1 million / year) and combines in-house developed peptide synthesizers, proprietary ultra-high-throughput quality control methods and large compound management capacities.
SPOT platform allows to produce small to large libraries of SIL peptides (individual or pooled) with unmatched pricing and delivery times on the market - Mid scale platform: JPT has developed since 2004 a unique mid scale peptide synthesis platform with a manufacturing capacity of 100,000 peptides annually at milligrams scale. This platform is highly versatile and allows to produce libraries of crudes up to Absolutely Quantified Peptides
- QTag : JPT has developed a unique absolute quantification technology allowing fast and reliable quantifications of tryptic peptides (presence of Arg or Lys at C-ter), becoming an alternative to Amino Acid Analysis which is more time-consuming.
Why choose JPT?
JPT Peptide Technologies is a trusted partner for stable isotope labeled peptides, combining decades of expertise with proprietary technologies to deliver high-quality, reliable solutions
- Proven expertise in SIL peptides synthesis with >20 years of experience
- Flexible production scales from micro scale discovery to mid scale manufacturing
- High isotopic enrichment (up to >95%) ensuring precise mass spectrometry performance
- Advanced proprietary platforms (SPOT & QTag) enabling high-throughput and precise quantification
- Custom and off-the-shelf solutions tailored to biomarker discovery, bioanalysis, and clinical research
- Key contributor to the Human Proteome Project through the ProteomeTools initiative, one of the largest proteomics efforts worldwide
- Synthesis of more than 1 million synthetic peptides covering essentially the entire human proteome, including modified peptides