Overlapping Peptide Library

use of the overlapping peptide scan

An overlapping peptide library is a collection of synthetic peptides that cover the entire sequence of a target protein, with each peptide overlapping partially with its neighboring sequences. This approach ensures complete and systematic coverage of the protein’s linear amino acid sequence, making it ideal for epitope mapping, T-cell and B-cell response studies, protein–protein interaction analysis, and functional domain identification.

By shifting each peptide by a few residues (typically 1–5 amino acids), overlapping peptide libraries allow researchers to pinpoint minimal binding regions or active sites with high resolution. Their precision and versatility make them essential tools in immunology, vaccine development, and proteomics research.

The Pioneer in Overlapping Peptide Library Technology


At JPT Peptide Technologies, we take pride in being pioneers in the development and production of overlapping peptide libraries. With decades of innovation and expertise, we've set the benchmark for peptide synthesis, intelligent library design, and high-throughput screening solutions. Our proprietary technologies, combined with an unwavering commitment to quality, have earned us the trust of researchers around the globe across immunology, infectious diseases, oncology, and personalized medicine.

Our overlapping peptide libraries are renowned for their precision, reproducibility, and flexibility empowering groundbreaking discoveries and accelerating scientific progress. To help you push the boundaries of your research, we also offer a wide range of customizable services tailored to your specific experimental needs. Explore our offerings below:

Available Overlapping Library Formats




Overlapping Peptide Libraries

Peptide Modifications

Micro-Scale Library

Amount: 10 to 100 μg per peptide
Purity: Crude
Delivery Format:  96 WP or Individual Tubes
Customized Modifications




Peptide Modifications

Milligram-Scale Libraries

Amount: 500 μg per peptide
Purity: Crude to 95%
Delivery Format:  96 WP or Individual Tubes 
Customized Modifications







 Overlapping Peptide Pools

Peptide Modifications

SpotMix Pools

Amount: 10 to 50 μg per peptide
Purity: Crude
Delivery Format:  96 WP or Individual Tubes
Best price on the market




Peptide Modifications

PepMix Pools

Amount: up to 200 peptides per pool
from 1mg  and higher
Purity: Crude to 95%
No false positive T-cell stimulation







Immobilized Overlapping Peptides

Peptide Modifications

Peptide Microarray

Peptides printed on array
Low to high density
Flexible microarray layout
Ideal for epitope mapping




Peptide Modifications

Peptide ELISA

Delivery Formats: 96 WP or 12x8 well strips
High batch-to-batch reproducibility
Ideal for epitope mapping
Cost-effective production




Design Your Own Piptide Library with PepSequencer

Designing an effective overlapping peptide library requires careful attention to peptide length, overlap size, and coverage strategy. At JPT, we leverage our proprietary PepSequencer designing tool to automate and optimize library design, ensuring high-quality, customized peptides tailored to your target protein. PepSequencer offers flexible customization to provide comprehensive coverage while minimizing redundancy and costs. With this powerful tool and our advanced synthesis platforms, JPT delivers precise, reproducible peptide libraries that accelerate your research.

Design Your Own Overlapping Peptide Library

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