Peptide Affinity Tags

What is a Peptide Affinity Tag?

One of the major challenges the production of recombinants is the purification of the target protein from other components expressed by the organism. To circumvent this, a recombinant protein is usually expressed with an additional amino acid sequence that allows for a specified subsequent affinity purification. Most common are the

Histidine tag: A string of histidine residues that binds to metal ions, often used for affinity chromatography.
FLAG-tag: A short sequence that binds to specific antibodies, ideal for detection and purification.
Strep-tag: Binds to streptavidin for high-affinity purification.

Once a tag is bound to the affinity resin, the protein is released, typically on an HPLC column. The release is facilitated by the excess addition of a competitive agent of the affinity tag.

JPT’s Peptide Affinity Tags

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JPT’s List of Peptide Affinity Tags

Here are some widely used peptide affinity tags, each serving unique purposes in protein purification, detection, and study:

FLAG Tag: A small octapeptide (DYKDDDDK), this tag binds to anti-FLAG antibodies, enabling purification and detection through immunoaffinity methods.
His Tag: A polyhistidine tag (usually 6-10 histidine residues) allows for purification via nickel or cobalt affinity chromatography.
Strep Tag (II): a small octapeptide (WSHPQFEK) that binds specifically to streptavidin or its derivatives, allowing for high-affinity purification.

Applications of Peptide Affinity Tags

Peptide affinity tags have a wide range of applications in molecular biology and biotechnology, supporting various experimental workflows:

Protein Purification: Peptide tags are essential in isolating recombinant proteins from complex mixtures.
Detection and Visualization: They can be used in Western blotting, ELISA, and immunofluorescence to detect proteins of interest.
Protein-Protein Interaction Studies: Peptide tags facilitate the study of interactions by allowing easy isolation of protein complexes.

JPT’s Custom Peptide Synthesis

JPT offers a range of peptide affinity tags that can be used as competitive agents for purification and elution of recombinant proteins. JPT can also produce customized affinity tag peptides, in bulk or other. Moreover, weoffer ISOplus and CGP grades that can be used in GMP manufacturing processes.

In addition to standard affinity tag peptides, JPT also offer custom production of peptide moieties for semisynthesis of proteins (suitable for various chemistry ligations).

Contact us for more information.