At JPT Peptide Technologies we understand the importance of reliability and efficiency in your research. Our line of Cell-Penetrating Peptides is designed to meet the highest standards of academic research. Explore our comprehensive selection of Cell Penetrating Peptides (CPPs), specifically designed for research and development in drug delivery, molecular biology, and therapeutic applications. CPPs offer unparalleled efficiency in transporting molecules across cell membranes, making them invaluable tools for advanced scientific research. Additionally, we offer conjugation of proteins, RNA lipids and more to CPPs and custom CPP synthesis services.
Why our CPP peptide services stand out:
1. Customizable Solutions: We offer a wide range of CPPs that can be tailored to meet the specific needs of your research. Whether you’re working with nucleic acids, proteins (e.g. fluorescent labeled peptides ), or other cargo, we can produce the CPP you need and conjugate to any chemical. Additionally, we offer the synthesis of precursor CPPs for further conjugations (e.g. maleimide moieties).
2. Expert Support: We understand that every research project is unique. That is why we offer not only high quality peptides but also dedicated technical support to help you optimize their use in your specific experiments.
3. Cost-Effective and Scalable: We offer competitive pricing without compromising on quality. Whether you are running small-scale exploratory studies or large-scale screenings, we have scalable solutions from µg to grams to match your needs.
4. Various purities: We typically offer We typically offer our CPPs peptides at >90% purity, but we can deliver any purity up to >95% according to your application requirements.
5. Conjugation Service: We offer conjugation of chemicls, RNA, DNA, as a service.
List of Cell Penetrating Peptides
Sort by
Cell-Penetrating Peptides (CPPs) as Delivery Systems
Researchers constantly seek efficient methods to introduce bioactive compounds, whether they be proteins, nucleic acids, or small molecules, into living cells without compromising their integrity. This is where Cell-Penetrating Peptides (CPPs) come into play, offering an innovative and highly effective solution for intracellular delivery.
What Are Cell Penetrating Peptides?
Cell Penetrating Peptides (CPPs) are short chains of amino acids capable of crossing cellular membranes, facilitating the delivery of various therapeutic and experimental cargoes, such as bioactive molecules, into cells. Discovered in the late 1980s, CPPs have since emerged as a versatile tool in cell biology, drug development, and therapeutic research. Their ability to transport molecules that otherwise cannot penetrate cell membranes, such as proteins, peptides, and even large nucleic acids like plasmid DNA or RNA, makes them indispensable for both fundamental research and clinical applications.
How CPPs Work
The Mechanism of Membrane Translocation The mechanism by which CPPs traverse cell membranes remains an area of active research, but two main pathways are recognized:
1. Endocytic Pathway: CPPs can initiate receptor-independent endocytosis, effectively encapsulating the cargo in endosomes, from which it can be released into the cytoplasm.
2. Direct Translocation: In some cases, CPPs interact directly with the lipid bilayer, forming transient pores or disrupting the membrane structure enough to allow the cargo to diffuse into the cell. These pathways allow CPPs to deliver cargo without the need for external delivery tools or harsh conditions, offering a gentler, more reliable approach compared to other delivery methods like electroporation or viral vectors.
Key Benefits of CPPs:
For academic researchers, CPPs are a game-changer. They provide a flexible platform for introducing molecules into a wide variety of cell types, from primary cells to cell lines, without the limitations of traditional methods. Here is why CPPs are increasingly becoming the go-to tool in the lab:- Versatile Delivery Vehicle: CPPs can be conjugated with nearly any type of molecule—proteins, peptides, nucleic acids, nanoparticles, and more—making them suitable for diverse applications in molecular and cellular biology.
- Non-Toxic and Non-Viral: CPPs are generally non-toxic to cells at working concentrations, offering a safer alternative to viral delivery systems that carry the risk of immunogenic responses.
- High Efficiency: CPPs have demonstrated remarkable efficiency in delivering macromolecules into cells, overcoming the challenge of cellular membrane impermeability, which has traditionally limited the use of many potential therapeutic agents.
- Broad Applicability: From gene editing and RNA interference studies to targeted drug delivery, CPPs have proven their worth in a wide array of research fields, including cancer biology, neuroscience, and regenerative medicine.
Types of Cell Penetrating Peptides
Explore our range of CPPs, categorized based on their structural and functional properties.Cationic CPPs:
Cationic CPPs are positively charged peptides, often composed of arginine or lysine residues. Their high affinity for negatively charged cell membranes makes them ideal for transporting various cargos into cells. Popular cationic peptides include TAT peptides and polyarginines, which are widely used in gene delivery and molecular research.
Features:
- High translocation efficiency
- Strong membrane interaction via electrostatic forces
- Ideal for delivering genetic material and proteins
Amphipathic CPPs:
Amphipathic peptides contain both hydrophilic and hydrophobic regions, allowing for interaction with both cell membranes and intracellular environments. These peptides are optimal for delivering hydrophobic drugs and have proven efficacy in crossing the blood-brain barrier.
Features:
- Dual nature of hydrophilic and hydrophobic regions
- Suitable for delivering hydrophobic and amphipathic molecules
- Stable membrane translocation without significant disruption
Hydrophobic CPPs
Hydrophobic CPPs are composed of nonpolar amino acids, making them particularly suited for delivering hydrophobic drugs into cells. While they have lower translocation efficiency than cationic or amphipathic peptides, they are essential for delivering substances that interact with lipid membranes.
Features:
- Nonpolar peptide structure
- Effective for the delivery of hydrophobic cargo
- Gentle interaction with cellular membranes.
Applications of Cell Penetrating Peptides
CPPs have revolutionized several fields of research, offering solutions to intracellular delivery challenges. Our selection of CPPs supports a range of applications:
Drug Delivery
CPPs can facilitate the intracellular delivery of small molecules, peptides, contrasting agents, metal chelates and proteins, making them invaluable for pharmaceutical research and drug development. They ensure that bioactive compounds reach their target within cells, improving efficacy and minimizing side effects.
Gene Therapy
CPPs are widely used for delivering nucleic acids like decoy DNA, RNA, siRNA, asONs and plasmids into cells. They are usually limited by pool cellular uptake due to high molecular weight and negative charges. This can be overcome by using CPP-nucleic acid conjugates.
Molecular Biology Research
In laboratories, CPPs are used to transport functional enzymes, fluorescent markers, and even antibodies into cells, enabling the study of cellular processes such as signal transduction, gene expression, and protein interactions. However, such proteins may also have therapeutic effects.
A list of the Most Commonly Known CPPs
- Tat peptide: Derived from the HIV-1 Tat protein, known for its ability to translocate across cell membranes.
- TAT-PTD: A modified version of the Tat peptide that enhances its cell-penetrating efficiency.
- Penetratin: A 16-amino acid peptide derived from the homeodomain of the Antennapedia protein in Drosophila, recognized for its efficiency in penetrating cells.
- Transportan: A fusion of a peptide from the neuropeptide galanin and the translocating peptide from the wasp venom, known for its ability to cross cell membranes.
- VP22: Derived from the herpes simplex virus, this peptide can spread through cells and is often used in gene delivery.
- R9: A poly-arginine peptide that has been widely studied for its cellular uptake properties. (also L9, H9 etc.)
- B18R: Derived from the vaccinia virus, it’s used for delivering various cargoes into cells.
- Ready-to-conjugate CPPs
Get in Touch for Bulk Orders or Custom Peptides
Cell-Penetrating Peptides are transforming the way researchers approach intracellular delivery, offering unparalleled flexibility and efficiency. With the growing demand for more targeted and efficient delivery systems, CPPs are set to become an even more critical tool for advancing academic research in areas such as drug discovery, gene therapy, and cellular imaging. At JPT, we are committed to providing the highest quality peptides to support your work. If you need custom peptides, CPP conjugates or bulk quantities, contact our team for a consultation.
