High Throughput Peptides


What is SPOT Synthesis?

The SPOT peptide synthesis technique is an ultra-high-throughput synthesis method for hundreds to thousands of peptides in parallel. Peptides are synthesized on a coated cellulose membrane that is used as solid phase. After synthesis peptides can remain on the membrane for an easy and inexpensive peptide array (PepSpots) or be separated from the mebrane and used as individual peptides (PepTrack Fast Track), pooled (SpotMix) or used to print on glass slides (PepStar).  

This automated process results in the production of huge numbers of peptides making this a fast and economical alternative to other methods that aim for deep T- and B-cell epitope discovery, MS-based proteomics relying on heavy & light reference peptides, antigen target identification and others.  

Our SPOT peptides come in many ready to use formats, which allow a broad application range for well-known experimental procedures with a robust readout.


Our High Throughput Peptide Formats

SpotMix (PLUS)
PepStar
PepSpots
PepTrack Fast Track (PLUS)
BioTides
FormatPeptide pools 96-well platesPeptides immobilized on glass slidePeptide spots bound on cellulose membraneIndividual peptides in 96-well platesIndividual peptides in 96-well plates
ApplicationsBroad antigen target discovery,
T-cell epitope discovery,
Biological screening,
Neo-epitope based immune monitoring
Biomarker discovery,
Humoral immune monitoring,
(Multiplexed) antibody epitope mapping,
detection of protein interactions
Biomarker discovery,
antibody epitope mapping,
detection of protein interactions
T-cell epitope discovery,
antigen target identification,
pathogen-spanning T-cell epitope discovery,
(neo-epitope prioritization)
Biomedical screening assays,
mapping of protein interactions, identification of enzyme substrates with standard screening systems
AssaysELISpotBinding assays with fluorescence read-outBinding assay with chemi-luminescence read-out
ELISpot
Binding assays with streptavidin coated beads, membranes, glass slides or microtiter plates (ELISA)
Peptide QC5% (or 100%) analyzed by LC-MS5% analyzed by LC-MS + control incubation of final microarray slide5% analyzed by MALDI-TOF-MS
5% (or 100%) analyzed by LC-MS

5% analyzed by LC-MS
Amount per peptide5-20 µg30 fmol 1-5 nmol
10 - 50 µg10 - 50 µg

References

References

References

  • A Group of Infection-Enhancing and Focus Size-Reducing Monoclonal Antibodies Recognized an ‘a and c’ Strands Epitope in the pr Domain of Dengue Virus prM
    Keelapang et al., Virus Research (2022)
  • Selection and Characterization of D-enantionmeric peptide ligands for polyglutamine proteins with therapeutic potential for polyglutamine milsfolding diseases
    Kolkwitz, Dissertation (2022)
  • Mass spectrometry-based draft of the mouse proteome
    Giansanti et al., Nature Methods (2022)
  • A context‑dependent and disordered ubiquitin‑binding motif
    Dreier et al., Cellular and Molecular Life Sciences (2022) - PMID: 35974206
  • Mechanistic basis of the increased methylation activity of the SETD2 protein lysine methyltransferase towards a designed super-substrate peptide
    Schnee et al., Communications Chemistry (2022) 
  • Active targeting of the extracellular matrix in the tumor microenvironment using novel collagen-binding peptides
    Veerman, Dissertation (2022)
  • Modulation der iNKT-(invariant natural killer T cells) Zell-Antwort durch das murine Cytomegalovirus
    Blaum, Dissertation (2022)
  • Immune checkpoint blockade augments changes within oncolytic virus-induced cancer MHC-I peptidome, creating novel antitumor CD8 T cell reactivities
    Kim et al., Molecular and Cellular Proteomics (2022)
  • Long Acting Capsid Inhibitor Protects Macaques From Repeat SHIV Challenges
    Vidal et al., Nature (2022)
  • Transcription factor EB sets the stage for fate decisions of antigen-primed B cells in the absence and presence of co-stimulation
    Wienands et al., Research Square (2022)
  • A Highly Specific Assay for the Detection of SARS-CoV-2–Reactive CD4+ and CD8+ T Cells in COVID-19 Patients
    Zelba et al., Journal of Immunology (2021)
  • Single Prime hAd5 Spike (S) + Nucleocapsid (N) Dual Antigen Vaccination of Healthy Volunteers Induces a Ten-Fold Increase in Mean S- and N- T-Cell Responses Equivalent to T-Cell Responses from Patients Previously Infected with SARS-CoV-2
    Sieling et al., MedRXiv (2021)
  • R1441G but not G2019S Mutation Enhances LRRK2 Mediated Rab10 Phosphorylation in Human Peripheral Blood Neutrophils
    Fan et al., ACTA Neurophathology (2021)
  • SARS-CoV-2 Protein Subunit Vaccination of Mice and Rhesus Macaques Elicits Potent and Durable Neutralizing Antibody Responses
    Mandolesi et al., Cell Rep Med (2021)
  • COVID-19 Subunit Vaccine with a Combination of TLR1/2 and TLR3 Agonists Induces Robust and Protective Immunity
    Jeong et al., Vaccine (2021)
  • The Choice of Search Engine Affects Sequencing Depth and HLA Class I Allele-Specific Peptide Repertoires
    Parker et al., Immunopeptidomics (2021)


More References
Application Notes
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