Internally Quenched Peptides / FRET Peptides


JPT’s internally quenched peptides or FRET peptides are guaranteed to be free of fluorescent impurities. The most frequently used dye/quencher combinations and common attachment options are shown below.


Fluorophore Quencher Excitation /
Emission Wavelength

Abz
2-Aminobenzoyl

Dnp
2,4-dinitrophenyl

320 nm
420 nm

Abz
2-Aminobenzoyl

NitroTyr
3-nitro-tyrosine

320 nm
420 nm

Mca
7-Methoxycoumarin-4-yl)acetyl

Dnp
2,4-dinitrophenyl

325 nm
392 nm

Trp
Tryptophan

Dnp
2,4-dinitrophenyl

280 nm
360 nm

EDANS
5-[(2-Aminoethyl) amino] naphthalene-1-sulfonic acid

Dabcyl
4-([4'-dimethylamino)phenyl]azo)benzoyl

340 nm
490 nm

FAM
Carboxyfluorescein

Dabcyl
4-([4'-dimethylamino)phenyl]azo)benzoyl

492 nm
517 nm

Lucifer Yellow

Dabsyl
4-(Dimethylamino)azobenzene-4'-sulfonyl

430 nm
520 nm



quenched FRET modifications
quenched FRET modifications
quenched FRET modifications