Fluorescent Peptides

Fluorescent Peptides & Dye Labeled Peptides

Dye and fluorescent labeled peptides are commonly used for protein binding or localization studies.

These labels can be incorporated at many positions in the fluorescent peptide or labeled peptide. Usually, they are incorporated at the N-terminus or at Cys/Lys side chains. Examples of frequently used fluorescent dyes and fluorescence labels and common attachment options for fluorescent labeled peptides are listed below.

Fluorophore Short Name	Full Name	Excitation / Emission Wavelength
Abz	Anthranilyl, 2-Aminobenzoyl	320 nm 420 nm
N-Me-Abz	N-Methyl-anthranilyl, N-Methyl-2-Aminobenzoyl	340-360 nm 440-450 nm
FITC	Fluoresceinisothiocyanate (5 or 6 isomer or mixture of isomers)	490 nm 520 nm
5-FAM	5-Carboxyfluorescein	492 nm 517 nm
6-FAM	6-Carboxyfluorescein	492 nm 517 nm
TAMRA	Carboxytetramethyl rhodamine (5 or 6 isomer or mixture of isomers)	543 nm 572 nm
Mca	7-Methoxycoumarinyl-4-acetyl	325 nm 392 nm
AMCA / Amc	Aminomethylcoumarin Acetate	350 nm 450 nm
Dansyl	5-(Dimethylamino) naphthalene-1-sulfonyl	342 nm 562 nm
EDANS	5-[(2-Aminoethyl)amino] naphthalene-1-sulfonic acid	340 nm 490 nm
Atto	e.g. Atto465, Atto488, Atto495, Atto550, Atto647	453 nm 508 nm (Atto465)
Cy3	1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride	550 nm 570 nm
Cy5	1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride	650 nm 670 nm
Alexa Fluor	e.g. Alexa Fluor 647, Alexa488, Alexa532, Alexa546, Alexa594, Alexa633, Alexa647	650 nm 668 nm (Alexa647)
Bodipy	e.g. Bodipy® FL	505 nm 511 nm
Dylight	e.g. DyLight 488, DyLight 550	493nm 518 nm (DyLight488)
Trp	Tryptophan	280 nm 360 nm
Lucifer Yellow (ethylene diamine)	6-Amino-2-(2-amino-ethyl)-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinoline-5,8-disulfonic acid	430 nm 520 nm

Fluorescence & Dye Labeled Peptides