We are happy to introduce a new assay to systematically explore protein-protein interactions:
Peptides are immobilized on a membrane that is incubated with a biological sample for affinity enrichment of soluble proteins and protein complexes. Subsequently, the membrane-bound peptides together with their binding partners are transferred into microtiter plates and subjected to trypsin. The digested proteins and protein complexes can then be identified and quantified by mass spectrometry.
Applications for Protein Interaction Screen on Peptide Matrix (PRISMA):
- Identification of protein-protein interaction partners
- Decode the influence of post-translational modifications
- Discovery of the influence of sequence diversity on protein interactions
- Comparison of cell states
- Mapping of pathways
Benefits of Protein Interaction Screen on Peptide Matrix (PRISMA):
- Hundreds of protein interactions can be detected and identified
- Modulatory effects of PTMs and mutations can be assessed
- Easy-to-use, efficient and reproducible alternative to other affinity enrichment approaches and immunoblotting analysis
We welcome your comments and are looking forward to discussing your study in detail!