Affinity Tag Peptides
Affinity tag peptides, also known as peptide tags or epitope tags, are short amino acid sequences fused to recombinant proteins to enable efficient protein detection, purification, and interaction analysis. These peptide tags are widely used in molecular biology workflows, including co-immunoprecipitation (Co-IP), Western blotting, and immunofluorescence. By binding to tag-specific antibodies or affinity ligands such as immobilized resins and magnetic beads, affinity tag peptides allow reliable isolation and characterization of target proteins.
JPT’s Affinity Tag Peptides
JPT Peptide Technologies offers a wide portfolio of high-quality affinity tag peptides engineered for robust and reproducible protein analysis. Our range includes commonly used tag formats such as FLAG tag peptides, His tag peptides, and V5 tag peptides. In addition, we offer reliable biotinylated variants to support diverse research applications in cancer, immunology, and vaccine development.
JPT also provides custom peptide synthesis tailored to your advanced molecular biology workflows.
How to Choose the Right Affinity Tag Peptide?
Selecting the right affinity tag peptide depends on the specific requirements of the experiment and the overall workflow. Key considerations include the intended application, the expression system, and compatibility with downstream analysis methods. In addition, factors such as tag size, chemical properties, potential impact on protein structure or function, and ease of purification should be taken into account. Ultimately, the optimal choice balances performance, reliability, and minimal interference with the target protein, ensuring consistent results across different experimental conditions.
Key properties of affinity tag peptides
The table below provides an overview of JPT’s affinity tag peptides and their key properties to support efficient selection for protein purification and detection workflows. Each tag binds to specific antibodies, metal ions, or affinity ligands such as immobilized resins or magnetic beads. This enables the analysis of tagged proteins via Western blotting, co-immunoprecipitation or other techniques.
| Tag Peptide | Sequence (aa) | Origin | Main Application | Key Properties |
| V5 | GKPIPNPLLGLDST (14 aa) | Simian virus 5 (paramyxovirus) | Detection with Western Blot/IF | Highly specific; trypsin resistant: works great for surface protein assays. |
| FLAG | DYKDDDDK (8 aa) | Synthetic | High-yield purification with Co-IP | Very hydrophilic and specific: unlikely to cause protein clumping. |
| HA | YPYDVPDYA (9 aa) | Influenza virus hemagglutinin | Imaging and localization with IF/Co-IP | Well validated; extreme affinity: binds tightly for sensitive imaging. |
| Myc | EQKLISEEDL (10 aa) | Human c-Myc protein | Expression Monitoring | Simple and widely used peptide tag for mammalian cells |
| His-tag | HHHHHH (6×His) | Synthetic | Bulk Purification (IMAC) | High binding capacity; works under denaturing conditions |
| Strep-tag | WSHPQFEK (8 aa) | Synthetic | High-purity affinity Purification | Gentle elution under mild conditions |
| T7 | MASMTGGQQMG (11 aa) | Bacteriophage T7 | Bacterial Expression and detection | Highly robust; optimized for bacterial systems |
| EPEA (C-tag) | EPEA (4 aa) | Human alpha-synuclein C-terminus | C-terminal Purification | Extremely small peptide tag; minimal protein interference |
| Rho1D4 | TETSQVAPA (9 aa) | Bovine rhodopsin C-terminus | Membrane Protein Purification | Highly specific and stabilizing for GPCR and membrane proteins |
| TY | EVHTNQDPLD (10 aa) | Yeast Ty1 retrotransposon | Detection (Western Blot) | Linear sequence; High-affinity for even low abundance proteins |
| VSV | YTDIEMNRLGK (11 aa) | Vesicular stomatitis virus glycoprotein | Intracellular Trafficking | Exceptional for intracellular tracking and live cell imaging |
| HA (Influenza A X-31) | YPYDVPDYA (9 aa) | Influenza A hemagglutinin (A/X-31 H3N2) | Viral Detection | Strain specific; allows tracking of specific flu variants. |
| SmBiT | VSGWRLFKKIS (11 aa) | NanoLuc split luciferase system | Measuring Real-time Interactions | Enables sensitive luminescence-based protein interaction assays |
| 3×FLAG | DYKDDDDKDYKDDDDKDYKDDDDK (24 aa) | Synthetic | Ultra-sensitive Detection/Co-IP | Strong antibody binding; enhanced detection sensitivity than FLAG |
Example illustrations of some epitope tag peptides are shown below:
Additional epitope tags can be synthesized upon request using our custom peptide synthesis services.
Research areas and applications
Affinity tag peptides are widely used by scientists in molecular and cellular biology to enable the purification, detection, and characterization of recombinant proteins. They support key research areas, including cancer, immunology, and drug discovery, where reliable protein analysis is essential.
Key applications include:
- Affinity purification: Isolation of tagged proteins using affinity chromatography
- Protein Detection: Analysis via techniques such as Western blotting, ELISA, and immunofluorescence to detect proteins of interest.
- Protein-Protein Interaction Studies: Identification of binding partners using pull-down assays and co-immunoprecipitation (Co-IP).
- Subcellular Localization: Visualization of proteins within cells using imaging techniques such as confocal microscopy or live-cell imaging.
- Functional and Translational Research: Characterization of protein function using assays such as reporter gene assays or enzyme activity assays.
- Drug and Vaccine Development: Support target validation, assay development, and biomarker discovery.
Why Choose JPT Affinity Tag Peptides?
JPT Peptide Technologies brings over 20 years of experience in peptide design and manufacturing, supporting researchers across academia, biotechnology, and pharmaceutical industries worldwide. With a proven track record of delivering millions of high-quality peptides, JPT has established itself as a trusted partner for reliable and reproducible peptide solutions. All our catalog peptides are provided with HPLC-MS analyses to confirm identity and ensure consistently high quality.
Benefits of JPT’s Peptides:
- High-throughput peptide synthesis for efficient and scalable production
- Manufactured in Germany under stringent quality standards
- Flexible ordering options, including bulk quantities and variable purities
- Freeze-dried aliquots available for enhanced stability and storage
- Proven expertise and dedicated scientific support
- Cost-effective and scalable solutions tailored to your needs
- Extensive modification options through custom peptide synthesis
Learn more about our affinity tag peptides!
Interested in high-quality tag peptides or need assistance selecting the right product for your research? Our reliable customer support team can support you with product information and custom peptide synthesis for modified/conjugated peptide designs. We also provide guidance on selecting the right anti-tag antibody for your application.
Please contact us or reach out to our scientific support team for personalized assistance. We are here to support your research and help you achieve reliable results.
Are you interested in other peptides for your research? Check out our extensive peptide catalog for more options!