Microarray combining an overlapping peptide scan through the full length sequence of HIV gag p17 and p24, tat, nef and env plus the remaining immunogenic regions of the HIV proteome. Each peptide is covered by at least one sequence from the most frequent clades ( A,B,C,D,G, CRF1,CRF2) of HIV 1. Coverage for all available sequences in the LANL alignment (2009) is for ENV 57%, GAG 72%, NEF 62% and TAT 46%.
Number of peptides: 6758 Amount: 1 microarray (1" x 3") Delivery Format: 1-sample peptide microarray Application(s): B-cell immunity Condition(s)/Topic(s): AIDS (HIV), HIV, Sequence diversity Standard Delivery Time: approx. 10 days
PepStar Antigen Collection HIV Ultra JPT Peptide Technologies applies its unique peptide microarray platform to generate peptide microarrays on glass slides displaying a versatile library (6758 15 mer peptides) with high clade coverage of the entire immunogenic HIV 1 proteome. It can be used for high resolution epitope mapping, immunomonitoring and biomarker discovery. Peptides are immobilized on glass slides via a flexible linker by chemoselective coupling. The covalently attached peptides are displayed in three copies on each slide. In addition, multiple identical copies of each microarray are produced. Incubation can be performed manually or automated using only minimal amounts of your sample. Read-out via fluorescence results in low background and high sensitivity.
Benefits of JPT's PepStar™ Microarrays - Screen proteome-wide in a cost, time and sample saving manner - Get hundreds of identical microarray copies - Peptides are free of truncated sequences - Small amounts of your precious samples are needed for incubation - Applicable to a variety of biological samples (e.g. serum, plasma, antibody) - Low background on glass surface - Read-out via fluorescence
References for PepStar™ Antigen Collection HIV Ultra
Application Notes A Modular Approach for Epitope Discovery and High-Resolution Profiling of Humoral Immune Responses Pawlowski et al. (2013) Full text Multiple Sclerosis and Epstein Barr Virus Infection. An Epitope Mapping Study Reimer et al. (2014) Full text The Challenge of Antigen Sequence Diversity:Solutions with ULTRA Peptide Libraries Pawlowski et al. (2015) Full text Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic antibody IMAB362 Daneschar et al. (2014) Full text
Testimonials for JPT's Peptide Microarrays "We at CNAM have studied the immune response of infected or control patients (Covid-19, asymptomatic, or pre-epidemic sera) by epitope mapping against peptides derived from the structural peptides of SARs-CoV-2. For our antibody mapping studies we have used the JPT's PepStar peptide microarrays and their comprehensive microarray profiling services. PepStar peptide microarrays with their very high quality and batch-to-batch reproducibility in combination with easy read-out and JPT’s personalized customer support have provided excellent results. We are very pleased to have worked with the JPT technology and plan to work again with them in the future.." Prof. Jean-Francois Zagury, Conservatoire National des Arts et Métiers, Paris, France
"We utilize JPT's multiwell microarray assay service to study autoimmune diseases such as rheumatoid arthritis. Our laboratory focuses on the mechanisms by which rheumatoid arthritis-associated HLA-DR molecules contribute to the development of anti-citrullinated protein antibodies (ACPAs) in rheumatoid arthritis. We have developed a sensitive peptide array assay, capable to detect and monitor ACPAs in mice (Front Immunol, 2022). JPT’s multiwell microarray assay service based on their PepStar peptide microarrays has been a most reliable and robust approach for our experiments. Their full profiling and data interpretation service with its excellent documentation has helped to further our research of rheumatoid arthritis.." Dr. Isabelle Auger, INSERM UMRs 1097, Marseille, France
"We study modulation of dopaminergic neurotransmisson by TAAR1 and D2R. The functional interaction of the two receptors in the brain supports TAAR1 as a target for the treatment of psychiatric disorders such as schizophrenia or bipolar disorder. To map the epitopes of our newly generated specific anti-rat TAAR1 antibodies we used JPT's PepStarTM peptide microarrays. The peptide microarrays greatly contributed to our successful and recently published study. We were very satisified with the exceptional product and service delivered by JPT Peptide Technologies as well as their scientific Customer Support which was always at our disposal." Stefan Obermüller, F.Hoffmann - La Roche Ltd., Roche Pharma Research and Early Development, Basel, Switzerland
Documentation for PepStar™ Antigen Collection HIV Ultra