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PM-VZV-gE

PepMix™ VZV (gE)

US$686.40

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About PepMix™ VZV (gE)

Glycoprotein Envelope from Varicella-Zoster Virus – HHV3

Glycoprotein Envelope (gE) is the most abundant protein on the surface of Varicella-Zoster Virus (VZV) - strain Dumas, also known as human herpesvirus 3 (HHV-3, HHV3). VZV gE or HHV3 gE is highly immunogenic and elicits strong specific IgG production. VZV encodes for several glycoproteins, including glycoprotein I (gI) which forms a heterodimer with gE to allow the virus to be active.

PepMix™ Peptide Pool VZV (gE)

The VZV gE peptide pool elicits specific T-cells stimulation including cytokine release and expression of specific markers. Thus, specific glycoprotein envelope T-cells can be detected, monitored or isolated and be useful for vaccine immunemonitoring, basic research, and more. PepMix™ VZV gE can be combined with PepMix™ VZV gI (PM-VZV-gI) for further assays.

Pool of 153 peptides derived from a peptide scan (15mers with 11 aa overlap) through Envelope protein (Swiss-Prot ID: P09259) of Varicella-Zoster Virus (VZV) - strain Dumas.

PepMix™ VZV (gE) - Specifications

  • Amount: 25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)
  • Purity: Development Grade: each peptide purified to > 70% (HPLC/MS) - (equivalent to an average purity of 85%)
  • Delivery Format: Freeze-dried in glass vial
  • Application(s): T-cell Immunity
  • Condition(s)/Topic(s): Chicken Pox, Herpes zoster, Shingles, Infection
  • Standard Delivery Time: 2-5 days

Customize your VZV gE pool

JPT can accommodate any specific request by preparing custom peptide pools with additional sequences, modifying the sequence design, delivering pools in specific formats (special aliquots, special tubes, plates, ready-to-use tubes...). High quality grades for clinical applications are also available. Contact us for inquiries about the production of custom peptide pools or visit Custom PepMix Peptide Pools.

About PepMix™ Peptide Pools

PepMix™ Peptide Pools
Protein-spanning mixtures of overlapping peptides (PepMix™) are extremely efficient for
- Antigen-specific T cell stimulation in T cell assays (e.g. in ELISpot, ICS, cell-mediated cytotoxicity or proliferation assays)
- In-vitro T cell expansion
- Dendritic cell pulsing
- Immune monitoring
- Cellular immune response profiling
- T cell epitope identification
- Standardization of T cell assays

Benefits of PepMix™
- Each peptide quality controlled and guaranteed for identity and purity
- CoA and HPLC-MS data available
- High batch-to-batch consistency
- No false positive T cell responses by contaminating deletion peptides
- No toxic inhibition of T cell responses due to stringent purification of each peptide
- Minimization of endotoxin contamination due to low bioburden process
- ADCF policy in place
- HLA independent stimulation (with antigen spanning peptide pools)

References for PepMix™ VZV (gE)

References
Read References with
PepMix™ Peptide Pools for Infectious Diseases
COVID-19 related PepMixes™
PepMix™ Control Pools

Application Notes
Cross-reactive T cells enhance immune responses in SARS-CoV-2 infection and vaccination
Loyal et al (2021) Full text
Peptide Tools to Support the Fight against COVID-19
Alem et al (2020) Full text
CEFX/EFX Ultra SuperStim Pools: Superior positive controls for T-cell assays with broad MHC-allele and antigen coverage
Eckey et al. (2017) Full text
Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy
Lam et al. (2015) Full text
Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation
Gary et al. (2015) Full text
PepMix™ Peptide Pools for Clinical Applications: T Cell Therapy for Viral Infections after Hematopoietic Stem Cell Transplant
Keirnan et al. (2012) Full text
Cytomegalovirus Protein Spanning PepMix™ Peptide Pools to Discover Changes in T-Cell Immunity in the Aging Population
Kern (2012) Full text

Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our go-to company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK

Documentation for PepMix™ VZV (gE)


Properties of PepMix™ VZV (gE)

Amount: 25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)
Application: T-cell immunity
Category: PepMix Peptide Pools, PepMix Peptide Pools Infections
Condition / Topic: Chicken Pox, Herpes zoster, Infection, Shingles
Layout: Freeze-dried in glass vial
Organism: Varicella-zoster virus (VZV)
Protein Name: Envelope protein
Purity: >70% (HPLC-MS), Development Grade: each peptide purified to > 70% (HPLC/MS)
Quantification: No

Further Information to PepMix™ VZV (gE)

Information Values
Sequence:
MGTVNKPVVGVLMGFGIITGTLRITNPVRASVLRYDDFHTDEDKLDTNSVYEPYYHSDHAESSWVNRGESSRKAYDHNSPYIWPRNDYDGFLENAHEHHGVYNQGRGIDSGERLMQPTQMSAQEDLGDDTGIHVIPTLNGDDRHKIVNVDQRQYGDVFKGDLNPKPQGQRLIEVSVEENHPFTLRAPIQRIYGVRYTETWSFLPSLTCTGDAAPAIQHICLKHTTCFQDVVVDVDCAENTKEDQLAEISYRFQGKKEADQPWIVVNTSTLFDELELDPPEIEPGVLKVLRTEKQYLGVYIWNMRGSDGTSTYATFLVTWKGDEKTRNPTPAVTPQPRGAEFHMWNYHSHVFSVGDTFSLAMHLQYKIHEAPFDLLLEWLYVPIDPTCQPMRLYSTCLYHPNAPQCLSHMNSGCTFTSPHLAQRVASTVYQNCEHADNYTAYCLGISHMEPSFGLILHDGGTTLKFVDTPESLSGLYVFVVYFNGHVEAVAYTVVSTVDHFVNAIEERGFPPTAGQPPATTKPKEITPVNPGTSPLLRYAAWTGGLAAVVLLCLVIFLICTAKRMRVKAYRVDKSPYNQSMYYAGLPVDDFEDSESTDTEEEFGNAIGGSHGGSSYTVYIDKTR
Specifications: Peptide scan (15mers with 11 aa overlap)