Pool of 28 peptides derived from a peptide scan (truncational scan (peptide length 11 to 17aa)) through Spike glycoprotein - Fusion peptide AA814-830 (Swiss-Prot ID: P0DTC2: 814 - 830) of SARS-CoV-2 (Severe Acute Respiratory Syndrome-Related Coronavirus 2).
PepMix™ SARS-CoV-2 (Spike Fusion-Peptide) - Specifications
Amount: 25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 10
8 cells)
Purity: Research Plus Grade: each peptide is guaranteed to be main product (HPLC/MS)
(equivalent to an average purity of 70%)
Delivery Format: Freeze-dried in glass vial
Application(s): T-cell Immunity
Condition(s)/Topic(s): Covid-19, Infection, Respiratory infection
Standard Delivery Time: 2-5 days
Background: A 15 amino acid stretch of the SARS-CoV-2 spike glycoprotein was recently shown to induce strong T-cell reactivity in individuals following SARS-CoV-2 vaccination and infection, and responses were also found in individuals with prior exposure to other human coronaviruses (Loyal et al., Science 2021). The amino acid stretch (named iCope) was independently predicted as a region of major antigenic potential in silico (Ong et al. Comp. Struct. Biotechnol. J. 2021).
The respective peptide sequence is located in the spike glycopeptide fusion domain and is likely to give rise to one or more universal immunodominant coronavirus-specific peptide epitopes across a range of presenting HLA-alleles.
Our PepMix peptide pool spans the region from SARS-CoV-2 Spike aa814 to aa830 including all possible 17-, 16-, 15-, 14, 13-, 12- and 11-amino acid peptides in order to maximize T cell stimulation.
Are you interested in your own custom PepMix? Choose your sequence, amount, purity and pool format. We will assist you along the way. Custom PepMix Peptide Pools.
PepMix™ Peptide Pools
Protein-spanning mixtures of overlapping peptides (PepMix™) are extremely efficient for
- Antigen-specific T cell stimulation in T cell assays (e.g. in ELISpot, ICS, cell-mediated cytotoxicity or proliferation assays)
- In-vitro T cell expansion
- Dendritic cell pulsing
- Immune monitoring
- Cellular immune response profiling
- T cell epitope identification
- Standardization of T cell assays
Benefits of PepMix™
- Production ISO 9001 certified
- Each peptide quality controlled and guaranteed for identity and purity
- CoA and HPLC-MS data available
- High batch-to-batch consistency
- No false positive T cell responses by contaminating deletion peptides
- No toxic inhibition of T cell responses due to stringent purification of each peptide
- Minimization of endotoxin contamination due to low bioburden process
- ADCF policy in place
- HLA independent stimulation (with antigen spanning peptide pools)
.jpg "PepMix™ SARS-CoV-2 (Spike Fusion-Peptide)")
.jpg "Epitope Mapping Peptide Set (EMPS) SARS-CoV-2 (Spike Glycoprotein)")
.jpg "PepMix™ SARS-CoV-2 (Spike B.1.617.2 / Delta)")
.jpg "PepMix™ SARS-CoV-2 (S-RBD B.1.617.2 / Delta)")
.jpg "PepMix™ SARS-CoV-2 (Spike Glycoprotein)")