Pool of 138 peptides derived from a peptide scan (15mers with 11 aa overlap) through 65 kDa phosphoprotein (pp65) (Swiss-Prot ID: P06725) of Human Cytomegalovirus (HCMV) - strain AD169.
PepMix™ Human CMV (pp65) peptide pools are essential tools for studying the immune response to CMV pp65, a major Cytomegalovirus protein critical in viral pathogenesis and immune recognition. This unique peptide pool stimulates T-cell responses, targeting both CD4 and CD8 T-cells across diverse MHC contexts (refer to table). These activated T-cells typically release pro-inflammatory cytokines, including interferon-gamma (IFN-γ), interleukin-2 (IL-2), and tumor necrosis factor (TNF), which play critical roles in modulating immune defenses against hCMV infection.
PepMix™ Human CMV (pp65) - Premium Grade - Specifications
- Amount: 25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)
- Purity: Premium Grade: each peptide purified to >90% (HPLC/MS) - (equivalent to an average purity of 95%)
- Delivery Format: Freeze-dried in glass vial
- Application(s): T-cell Immunity
- Condition(s)/Topic(s): Control, Opportunistic infections, Cytomegalovirus disease, Infection
- Standard Delivery Time: 2-5 days
Premium Grade Quality PepMix™
For applications where performance, reproducibility, and stability are critical, our new Premium Grade PepMixes™ feature multiple layers of ongoing quality control:
- Recurrent Performance TestingEach batch is regularly tested via ELISpot assays on two donors. Your Benefits:
- Assay standardization & validation
- Minimized performance variability
- Updated Certificate of analysis (CoA)
- Cytotox TestingEach batch is tested in an in vitro Resazurin-based assay to determine if the dissolved peptides show cytotoxic effects.
- Endotoxin TestingPh. Eur.2.6.14A LAL test or Haemotox rFC test are performed with each batch to detect endotoxins.
- Sterility TestingPh. Eur.2.6.1Each batch is incubated in tryptic soy broth. Later turbidity is determined as indicator of contaminating microorganisms.
- Plus, our trusted quality benchmarks still apply
- > 90% Purity of each peptide in the pool (via HPLC-MS)
- Validated pooling method ensuring the presence of each peptide
- Produced under stringent quality management
- CoA and HPLC-MS data available for each individual peptide batch
- Avoiding false positive T-cell responses by contaminating deletion peptides
- ADCF policy
The Role of CMV pp65 in hCMV Infection and Host Immunity
The pp65 protein, a prominent tegument protein in Human Cytomegalovirus (HCMV), plays an integral role in viral replication, immune evasion, and host cell infection. As a key structural protein, it facilitates the assembly of viral particles, modulates viral gene expression, and impacts the virus-host interaction during the early stages of infection.
CMV pp65 is critical in shaping the immune landscape, acting as a dominant target for both innate immunity and adaptive T-cell responses. Its ability to stimulate infected cell recognition makes it an invaluable tool for studying viral genome expression and the immune surveillance mechanisms in various cell types.
PepMix™ Peptide Pool Human CMV pp65 Applications
The PepMix™ Human CMV (pp65) antigen detects T-cell responsiveness to HCMV. As most people have CMV-specific T-cells recognizing HCMV pp65 the PepMix™ Human CMV (pp65) is often used as a positive control in T-cell stimulation assays. It is also useful for isolating CD4 and CD8 T-cells from PBMCs after activation. This isolation uses antibody-labeled magnetic beads. HCMV pp65-specific T-cells can be enriched through peptide stimulation and proliferation, creating T-cell lines or clones. JPT’s PepMix™ Peptide Pool Human CMV (pp65) is suitable for various cell assays needing high-quality peptides, including cytokine production-dependent assays like ELISPOT or flow cytometry, and detection of surface activation markers such as anti-CD154 or anti-CD137. Unlike non-specific controls such as as staphylococcus enterotoxin B (SEB), phorbol-12-myristate 13-acetate(PMA)/Ionomycin, or phytohemagglutinin (PHA), PepMix™ provides a T-cell receptor-dependent activation control, making it a more physiological and relevant option for peptide-based T-cell activation assays.
HLA Specificty of PepMix™ Peptide Pool Human CMV pp65
Peptides from the CMV pp65 protein are known to be presented by a wide range of HLA-molecules (note that some studies reported low-resolution, serologic specificities whereas others reported specific alleles). JPT can provide individual T-cell stimulating CMV-derived pp65 peptides for each mentioned allele. MHC molecules presenting peptides derived from human CMV-pp65 (see table).
Applications of PepMix™ Human CMV (pp65)
Detection and Functional Analysis
The PepMix™ Human CMV (pp65) antigen is a powerful tool for:
- Detecting T-cell responsiveness to HCMV, a common issue during HCMV infection.
- Using as a positive control in T-cell stimulation assays due to its ability to stimulate CMV-specific T-cells effectively.
Isolation and Enrichment of T-CellsThis product facilitates the isolation of CD4 and CD8 T-cells from PBMCs (Peripheral Blood Mononuclear Cells) through activation-dependent processes. By leveraging antibody-labeled magnetic beads, HCMV pp65-specific T-cells can be enriched and expanded, enabling the creation of T-cell lines or clones for in-depth studies.
Advanced ApplicationsJPT’s PepMix™ Human CMV (pp65) is ideal for high-quality cell assays such as:
- Cytokine production-dependent assays, including ELISPOT and flow cytometry
- Detection of surface activation markers such as anti-CD154 or anti-CD137.
Unlike non-specific controls like staphylococcus enterotoxin B (SEB) or phytohemagglutinin (PHA), the PepMix™ pool ensures T-cell receptor-dependent activation, providing a more physiological and reliable solution for peptide-based T-cell activation assays.Visit our webpage for more information on Peptide Tools to Study CMV.
Custom Solutions for Specialized Research
Looking for a tailored solution? Custom PepMix™ peptide pools and individual HLA-specific peptides provide flexibility for:
- Exploring novel host-virus interactions in hCMV infection.
- Standardizing T-cell assays for cross-laboratory consistency.
- Developing advanced tools for immune monitoring in diverse patient cohorts.
PepMix™ Peptide PoolsProtein-spanning mixtures of overlapping peptides (PepMix™) are extremely efficient for
- Antigen-specific T cell stimulation in T cell assays (e.g. in ELISpot, ICS, cell-mediated cytotoxicity or proliferation assays)
- In-vitro T cell expansion
- Dendritic cell pulsing
- Immune monitoring
- Cellular immune response profiling
- T cell epitope identification
- Standardization of T cell assays
Advancing Research on hCMV Genes and T-cell Responses
Harnessing the versatility of PepMix™ Human CMV pp65 enables researchers to:
- Decode interactions between hCMV replication and host cells.
- Study viral genome dynamics in infected and wild-type cells.
- Identify and characterize T-cell epitopes for vaccine development and therapeutic interventions.
Benefits of PepMix™
- Each peptide quality controlled and guaranteed for identity and purity
- CoA and HPLC-MS data available
- High batch-to-batch consistency
- No false positive T cell responses by contaminating deletion peptides
- No toxic inhibition of T cell responses due to stringent purification of each peptide
- Minimization of endotoxin contamination due to low bioburden process
- ADCF policy in place
- HLA independent stimulation (with antigen spanning peptide pools)
Unique Benefits of PepMix™ for hCMV Research
PepMix™ peptide pools stand apart for their unmatched reliability and precision in T-cell receptor-dependent activation. Key benefits include:
- HLA-independent stimulation, ensuring broader applicability across MHC molecules.
- Superior batch-to-batch consistency for reproducible results.
- Minimal endotoxin contamination, preserving innate immunity response fidelity.
- Fully documented quality assurance, including CoA and HPLC-MS data.
