PM-PSPL
Positional scanning peptide library with 181 different peptide mixtures to identify stimulating peptides for T-cell clones. Each mixture contains a very large number of 9mer peptides that are identical with respect to one single, fixed amino acid position but are variable with respect to all other positions.
Additional information on generation and use of Positional Scanning libraries
Are you interested in your own custom PepMix? Choose your sequence, amount, purity and pool format. We will assist you along the way. Custom PepMix Peptide Pools.
PepMaster™ Positional Scanning Peptide Library
PepMaster™ offers 181 different peptide mixtures to identify stimulating peptides for T-cell clones. Each mixture contains a very large number of 9mer peptides that are identical with respect to one single, fixed amino acid position but are variable with respect to all other positions. This creates peptides, for example, of the format ‘A1-X2-X3-X4-X5-X6-X7-X8-X9’, ‘X1-A2-X3-X4-X5-X6-X7-X8-X9, ‘X1-X2-A3-X4-X5-X6-X7-X8-X9’, etc., where ‘A’ represents alanine, and ‘X’ represents any amino acid except cysteine (to prevent oligomerization or cyclization). The ability of a specific T-cell receptor (TCR) to recognise, for example, the ‘X1-A2-X3-X4-X5-X6-X7-X8-X9’ library depends on the preference of this TCR for ‘A’ in position 2 of the peptide chain. PepMaster™ is used like a PepMix™, however, the ideal concentrations should be determined as they depend on the purpose and design of the experiments.
Benefits of PepMix™
- Each peptide quality controlled and guaranteed for identity and purity
- CoA and HPLC-MS data available
- High batch-to-batch consistency
- No false positive T cell responses by contaminating deletion peptides
- No toxic inhibition of T cell responses due to stringent purification of each peptide
- Minimization of endotoxin contamination due to low bioburden process
- ADCF policy in place
- HLA independent stimulation (with antigen spanning peptide pools)
References:
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Application Notes
Cross-reactive T cells enhance immune responses in SARS-CoV-2 infection and vaccination
Loyal et al (2021) Full text
Peptide Tools to Support the Fight against COVID-19
Alem et al (2020) Full text
CEFX/EFX Ultra SuperStim Pools: Superior positive controls for T-cell assays with broad MHC-allele and antigen coverage
Eckey et al. (2017) Full text
Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy
Lam et al. (2015) Full text
Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation
Gary et al. (2015) Full text
PepMix™ Peptide Pools for Clinical Applications: T Cell Therapy for Viral Infections after Hematopoietic Stem Cell Transplant
Keirnan et al. (2012) Full text
Cytomegalovirus Protein Spanning PepMixTM Peptide Pools to Discover Changes in T-Cell Immunity in the Aging Population
Kern (2012) Full text
Properties | Values |
---|---|
Amount: | 440 µg /pool |
Application: | Enzymology, T-cell immunity |
Category: | PepMix Peptide Pools, PepMix Peptide Pools other Antigens |
Condition / Topic: | Autoimmune disease, Cancer, Control, Infection |
Layout: | 96 well microtiter plate |
Organism: | Other/None |
Protein Name: | Other |
Purity: | crude |
Quantification: | No, Yes |
Information | Values |
---|---|
Specifications: | 9mer peptides |
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