PepMaster™ Positional Scanning Peptide Library

Positional scanning peptide library with 181 different peptide mixtures to identify stimulating peptides for T-cell clones. Each mixture contains a very large number of 9mer peptides that are identical with respect to one single, fixed amino acid position but are variable with respect to all other positions.

Amount: 440 µg /pool
Purity: crude
Delivery Format: 96 well microtiter plate
Application(s): Antigen specific T-cell stimulation, Immune monitoring, Standardization of T cell assays, Substrate identification, T-cell assays, T-cell expansion
Indication(s)/Topic(s): Autoimmune disease, Cancer, Control, Infection
Delivery Time: 2-5 days

Additional information on generation and use of Positional Scanning libraries

PepMaster™ Positional Scanning Peptide Library
PepMaster™ offers 181 different peptide mixtures to identify stimulating peptides for T-cell clones. Each mixture contains a very large number of 9mer peptides that are identical with respect to one single, fixed amino acid position but are variable with respect to all other positions. This creates peptides, for example, of the format ‘A1-X2-X3-X4-X5-X6-X7-X8-X9’, ‘X1-A2-X3-X4-X5-X6-X7-X8-X9, ‘X1-X2-A3-X4-X5-X6-X7-X8-X9’, etc., where ‘A’ represents alanine, and ‘X’ represents any amino acid except cysteine (to prevent oligomerization or cyclization). The ability of a specific T-cell receptor (TCR) to recognise, for example, the ‘X1-A2-X3-X4-X5-X6-X7-X8-X9’ library depends on the preference of this TCR for ‘A’ in position 2 of the peptide chain. PepMaster™ is used like a PepMix™, however, the ideal concentrations should be determined as they depend on the purpose and design of the experiments.

Benefits of JPT’s PepMaster™ Positional Scanning Peptide Library
- Effective tool to measure T cell specificity
- Whole production performed in a regulated European environment according to ISO 9001:2015
- Synthesis protocols designed to avoid toxic contaminants and side products