Positional scanning peptide library with 181 different peptide mixtures to identify stimulating peptides for T-cell clones. Each mixture contains a very large number of 9mer peptides that are identical with respect to one single, fixed amino acid position but are variable with respect to all other positions.
PepMaster Positional Scanning Peptide Library - Specifications
- Amount: 440 µg /pool
- Purity: crude
- Delivery Format: 96 well microtiter plate
- Application(s): Enzymology, T-cell Immunity
- Condition(s)/Topic(s): Autoimmune disease, Cancer, Control, Infection
- Standard Delivery Time: 2-5 days
Additional information on generation and use of Positional Scanning libraries
Are you interested in your own custom PepMix? Choose your sequence, amount, purity and pool format. We will assist you along the way. Custom PepMix Peptide Pools.
PepMaster™ Positional Scanning Peptide Library
PepMaster™ offers 181 different peptide mixtures to identify stimulating peptides for T-cell clones. Each mixture contains a very large number of 9mer peptides that are identical with respect to one single, fixed amino acid position but are variable with respect to all other positions. This creates peptides, for example, of the format ‘A1-X2-X3-X4-X5-X6-X7-X8-X9’, ‘X1-A2-X3-X4-X5-X6-X7-X8-X9, ‘X1-X2-A3-X4-X5-X6-X7-X8-X9’, etc., where ‘A’ represents alanine, and ‘X’ represents any amino acid except cysteine (to prevent oligomerization or cyclization). The ability of a specific T-cell receptor (TCR) to recognise, for example, the ‘X1-A2-X3-X4-X5-X6-X7-X8-X9’ library depends on the preference of this TCR for ‘A’ in position 2 of the peptide chain. PepMaster™ is used like a PepMix™, however, the ideal concentrations should be determined as they depend on the purpose and design of the experiments.
Benefits of PepMix™
- Each peptide quality controlled and guaranteed for identity and purity
- CoA and HPLC-MS data available
- High batch-to-batch consistency
- No false positive T cell responses by contaminating deletion peptides
- No toxic inhibition of T cell responses due to stringent purification of each peptide
- Minimization of endotoxin contamination due to low bioburden process
- ADCF policy in place
- HLA independent stimulation (with antigen spanning peptide pools)
