MHC I & II Multimers

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T cells recognize specific MHC-peptide complex displayed on the surface of antigen-presenting cells. This specific interaction is used by MHC multimers carrying MHC-peptide complexes to detect and isolate antigen-specific T cell. Fluorescent-labeled MHC multimers are used to detect, quantify and isolate antigen-specific T cells in fluid samples (e.g. blood, cultured cell lines, CSF, lymph, synovial fluid) by flow cytometry, and for in situ detection (e.g. in solid tumors) by immunohistochemistry (IHC).

Find the related Antigen Peptide for each MHC Multimer or order your antigen by custom peptide synthesis!

MHC Dextramers®

Stabilizing dextran polymer backbone
Fluorochrome molecules
More MHC-peptide complexes than conventional MHC multimers (such as tetramers)
Stronger staining intensity
Increased resolution and signal-to-noise ratio

MHC Dextramers detect the presence of antigen-specific T cells whereas interferon-gamma-releasing assays such as ELISpot detect the functionality of the antigen-specific T cells. Thus, a combination of the MHC Dextramer assay and an interferon-gamma-releasing assay, using PepMix™ Peptide Pools as specific stimulating antigens, is an effective tool for reliable and robust immune monitoring!

Individual MHC I Dextramers®

More than 400 different MHC I Dextramers® are available!

Selection of available Alleles:

Human alleles		Mouse alleles	Monkey alleles
HLA-A*0101	HLA-B*0702	H-2 Db	Mamu-A*01
HLA-A*0201	HLA-B*0801	H-2 Dd	Mamu-A*04
HLA-A*0301	HLA-B*2705	H-2 Dk	Mamu-A*08
HLA-A*1101	HLA-B*3501	H-2 Kb	Mamu-B*17
HLA-A*2301	HLA-B*4201	H-2 Kd
HLA-A*2402	HLA-B*4403	H-2 Kk
HLA-A*2902	HLA-B*5101	H-2 Ld
HLA-A*6801	HLA-B*5701
	HLA-B*5703
	HLA-CW*0304

Individual MHC II Dextramers®

Fluorescent MHC II multimers available with three different fluorochromes and many alleles, e.g.

DPB1*0401

DRB1*0101

DRB1*0401

DRB1*1101

MHC I Dextramer® Collections

MHC I Multimer Collections are carefully selected sets of multimers (Dextramers®) for staining and isolation of antigen-specific CD8+ T cells. They are available for a variety of viruses and tumors with different fluorochromes (APC, PE, FITC), e.g.

CMV
EBV
Melanoma
Cancer-testis antigens
Virus antigens

Custom MHC Mutlimers

Fast delivery of custom MHC Dextramers® upon request.

Applications

Applications for MHC I & II Multimers

Detection, enumeration and isolation of antigen-specific T cells in fluid cell samples (PBMC, blood) by flow cytometry
In vitro staining of tissue sections to visualize antigen-specific T cells
Vaccine and immunotherapeutic development
Clinical trials
Immune monitoring of e.g. transplant patients and HIV/AIDS patients

Benefits

Benefits of MHC I & II Multimers

More sensitive than conventional MHC multimers (important for low-affinity interactions commonly encountered in cancer & autoimmune disease)
Very stable
Order the related Antigen Peptide together with your MHC Dextramer
Customized Dextramers® available upon request

References

Quality Assurance

All production is performed according to ISO 9001:2015 standards.

MHC Multimers