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Vorinostat Renders the Replication-Competent Latent Reservoir of Human Immunodeficiency Virus (HIV) Vulnerable to Clearance by CD8 T Cells

Sung et al., EBioMedicine (2017) - PMID: 28803740

Product(s) used in this publication: PepMix™ Peptide Pools

Abstract

Latently humanimmunodeficiencyvirus (HIV)-infected cells are transcriptionally quiescent and invisible to clearance by the immune system. To demonstrate that the latency reversing agent vorinostat (VOR) induces a window of vulnerability in the latentHIVreservoir, defined as the triggering of viral antigen production sufficient in quantity and duration to allow for recognition and clearance of persisting infection, we developed a latency clearance assay (LCA). The LCA is a quantitative viral outgrowth assay (QVOA) that includes the addition of immune effectors capable of clearing cells expressing viral antigen. Here we show a reduction in the recovery of replication-competentvirus from VOR exposed resting CD4 Tcells following addition of immune effectors for a discrete period.

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