Evolution of Antibodies to Epitopes of PE_PGRS51 in the Spectrum of Active Pulmonary Tuberculosis

Products & Services
Catalog
- PepMix™ Peptide Pools
  PepMix™ Peptide Pools
  Pools of overlapping peptides from antigenic proteins for T-cell stimulation, T-cell expansion in T-cell assays such as ELISPOT, ICS or Flow Cytometry, e.g. for immune monitoring.
- Transduction Enhancer
  
  Transduction Enhancer
  Protransduzin™ the highly efficient transduction enhancer increases viral gene transfer in pre-clinical and clinical applications, such as ex vivo gene therapy and CAR-T cell development.
- Antigen Peptides
  - COVID-19 Antigen Peptides
  - Further Antigen Peptides
  Antigen Peptides
  Antigen peptides represent specific epitopes for stimulation of T cells in T cell assays such as ELISPOT. We offer the corresponding MHC multimer for each antigen peptide. Antigens from different pathogens are available as well as tumor associated antigens.
- MHC Multimers
  MHC Multimers
  MHC Multimers for reproducible detection, enumeration and isolation of antigen-specific T cells with disease specific peptides.
- Proteomics Peptides & Kits
  Proteomics Peptides & Kits
  Peptide sets and pools, as well as assay standardization kits are available with stable isotope labeled or unlabeled proteotypic peptides for mass-spectrometry based proteomics such as MRM assays.
- RepliTope™ Peptide Microarrays
  RepliTope™ Peptide Microarrays
  Peptide microarrays that display overlapping peptide scans through antigens from infectious organisms or tumor associated antigens for antibody or serum profiling.
- Chelate Peptides (DOTA)
  
  Chelate Peptides (DOTA)
  DOTA is linked to molecules that have affinity for various structures (e.g. somatostatin receptors in neuroendocrine tumors). The resulting compounds can be bound to radionuclided and are used with a number of radioisotopes in cancer therapy and diagnosis
- Cosmetic Peptides (DPRA Peptides)
  
  Cosmetic Peptides
  Cosmetic Peptides such as Lysine and Cysteine Peptide are used for DPRA (Direct Peptide Reactivity Assay) for Skin Sensitization Testing. The DPRA measures the reaction of a chemical with synthetic peptides containing Cysteine (Ac‑RFAACAA‑COOH) or Lysine (Ac‑RFAAKAA‑COOH) to assess its sensitization potency. For research use only!
- Immunology Standards & Controls
  - Control Peptide Pools
  - TERS Reference Samples
  Immunology Standards & Controls
  Standards and controls for reproducible T-cell assays such as ELISPOT and multimer assays. We offer a large variety of positive and negative control peptide pools for antigen specific T cell stimulation as well as kit to produce TCR-engineered reference samples for performance control.
- Abeta & Prion Peptides
  - Amyloid Beta A4 Peptides
  - Major Prion Peptides
  Abeta & Prion Peptides
  Purified, fully analyzed, high quality peptides, synthesized from the peptide experts for Alzheimers and prion research.
- Assay Services
  
  Assay Services
  We offer our extensive know-how and experience in peptide microarray incubation & data evaluation as full-service packages for many applications.
- Enzyme Substrates
  Enzyme Substrates
  Large collections of peptidic enzyme substrates in microtiter plates or displayed on microarrays for screening and enyzme profiling.
- Histone Peptide Tools
  Histone Peptide Tools
  Our histone peptide library is the largest on the market and available as Peptide ELISA, Peptide Microarray or sets of histone peptides
Contract Research
Company
- - JPT has a work force of >100 highly trained and skilled employees. 30 of our co-workers hold a university degree and 20 have a PhD in natural sciences.
News & Publications
Support & Contact
- - We take pride in our competent service and swift response. Please do not hesitate to contact us for further information. We also very much welcome your feedback and comments.

Evolution of Antibodies to Epitopes of PE_PGRS51 in the Spectrum of Active Pulmonary Tuberculosis

Rama Murthy Sakamuri et al., Accepted manuscript (2019) - PMID: 31641771

Product(s) used in this publication: Peptide ELISA

Abstract

BACKGROUND:

The PE_PGRS protein family isimplicated in virulence and immunopathogenesis of M. tuberculosis. Due to extensive intra-family homology, it has not been possible to correlate expression of specific members with stage of infection or disease progression. Here, we investigate the in vivo expression of PE_PGRS51, which besides the cross-reactive PE and PGRS domains, has 3 unique regions.

METHODS:

Expression in M. smegmatis confirmed PE_PGRS51 cell-wall localization. Patients at different stages of TB were classified across a spectrum akin to the Leprosy spectrum. Peptide arrays representing PE_PGRS51 were screened with sera from TB patients across the spectrum of active TB, with latent TB sera as controls.

RESULTS:

Antibodies directed only against conserved epitopes within the PE/PGRS domains were detectable at early stages of TB. As bacteriological burden and disease progresses, the epitope repertoire extends systematically to adjacent and overlapping peptides. Sera-reactivity to epitopes unique to PE_PGRS51 appear only in the most advanced TB patients, indicating PE_PGRS51 expression in vivo.

DISCUSSION:

The ability to classify active TB patients into a spectrum and delineate the in vivo expression of PE_PGRS proteins at different stages of disease has important implications for understanding their role in TB pathogenesis and their usefulness as diagnostic markers.

Search

All Our Publications