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Wilms’ Tumor 1-specific Cytotoxic T Lymphocytes Can be Expanded from Adult Donors and Cord Blood

Krishnadas et al., Leukemia Research (2011) - PMID: 21774984

Product(s) used in this publication:  Specialty Peptides

Abstract:

The use of WT1-specific CTL is one potential strategy to treat leukemic relapse following allogeneic stem cell transplant (SCT). Previous studies have largely focused on generating WT1-CTL from adult donors by cloning. We demonstrate that WT1-CTL can be generated from healthy adult donors and from cord blood by stimulating with an overlapping pool of peptides derived from full length WT1 and selecting antigen-specific cells based on the expression of CD137. The rapid expansion with anti-CD3 and IL-2 resulted in a 100-200-fold expansion. These CTL lysed WT1 expressing targets, including leukemia lines, in a HLA restricted manner.

Copyright © 2011 Elsevier Ltd. All rights reserved.

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