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Relationships Between T Cell and IgE/IgG4 Epitopes of the Anisakis Simplex Major Allergen Ani s 1

Alonso et al., Clin Exp Allergy. (2015) - PMID: 25495594

Product(s) used in this publication:  PepStar™ Peptide Microarrays

Abstract:

BACKGROUND:

Anisakiasis is a global disease caused by the consumption of raw or lightly cooked fish parasitized with third-stage Anisakis larvae. Anisakis simplex allergens may cause severe allergic reactions including angio-oedema, urticaria and anaphylaxis. Approximately 80% of allergic patients have allergen-specific IgE against Ani s 1, and the diagnostic value of testing for antibodies to Ani s 1 has been extensively demonstrated. However, no previous studies have investigated the molecular aspects of the allergic response to Ani s 1. Knowledge of allergen-specific T cell and B cell (IgE and IgG4) epitopes is important for elucidating the immunological mechanisms underlying allergic responses, and for understanding why particular proteins behave as allergens.

OBJECTIVE:

To elucidate the main T cell- and B cell (IgE and IgG4)- binding regions of Ani s 1.

METHODS:

T cell epitopes were identified by peptide proliferation assays using T cell lines derived from peripheral blood mononuclear cells of 11 patients with Anisakis allergy, and IgE and IgG4 epitopes were identified by microarray immunoassay using sera from a different group of 11 patients with Anisakis allergy.

RESULTS:

Several T cell epitopes of Ani s 1 were identified, of which Ani s 1145-156 , Ani s 1151-162 and Ani s 1163-171 located at the C-terminal end of the protein were the most relevant. IgE and IgG4 recognized largely the same peptides, including Ani s 122-41 , Ani s 125-44 , Ani s 127-47 , Ani s 137-56 and Ani s 194-113 .

CONCLUSIONS AND CLINICAL RELEVANCE:

This is the first report describing the T cell epitopes of an important allergen of A. simplex, and the first B cell epitope study of this allergen in the Spanish population. This information can help to elucidate the mechanisms underlying the allergic response to Ani s 1, potentially leading to therapeutic and diagnostic advances.

© 2014 John Wiley & Sons Ltd.

KEYWORDS:

Anisakis allergy; B cell epitopes; Kunitz-type serine protein inhibitors; T cell epitopes; epitope mapping; recombinant Ani s 1

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