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High-Throughput Microarray Incubations Using Multi-Well Chambers

Zerweck et al., Methods Mol Biol. (2015) - PMID: 26490464

Product(s) used in this publication:  PepStar™ Peptide Microarrays

Abstract:

Peptide microarrays are ideal tools for a variety of applications ranging from epitope mapping to immune monitoring. Here we present a method for high-throughput screening of biological samples using only standard microtiter plate equipment. Parallel incubation of a large number of samples with a small library of peptides is enabled by printing multiple identical mini-arrays on one microarray slide and further combining four slides to yield an incubation frame possessing the dimensions of a 96-well microtiter plate. Applying conventional lab equipment such as ELISA washers, hundreds of samples can be processed in 1 day yielding approx. 200 data points in triplicates per sample.

KEYWORDS:

Biomarker verification; High-throughput screening; Multi-well incubation chamber; Peptide microarray

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