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High-Density Peptide Arrays for Malaria Vaccine Development

Loeffler et al., Methods Mol Biol. - Book (2016) - PMID: 27076154

Product(s) used in this publication:  PepStar™ Peptide Microarrays

Abstract:

The development of an efficacious and practicable vaccine conferring sterile immunity towards a Plasmodium infection represents a not yet achieved goal. A crucial factor for the impact of a given anti-plasmodial subunit vaccine is the identification of the most potent parasitic components required to induce protection from both infection and disease. Here, we present a method based on a novel high-density peptide array technology that allows for a flexible readout of malaria antibodies. Peptide arrays applied as a screening method can be used to identify novel immunogenic antibody epitopes under a large number of potential antigens/peptides. Ultimately, discovered antigen candidates and/or epitope sequences can be translated into vaccine prototype design. The technology can be further utilized to unravel antibody-mediated immune responses (e.g., involved in the establishment of semi-immunity) and moreover to confirm vaccine potency during the process of clinical development by verifying the induced antibody responses following vaccination.

KEYWORDS:

Antibody epitope mapping; Antibody readout; Antigen screening; Immunogenic malarial antigens; Malarial subunit vaccine; Peptide binding

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