Peptide Tools to Study HCMV

About Human Cytomegalovirus

HCMV or HHV-5 belongs to the family of Herpesviridae and is known to establish latency in leukocytes. At least 40% of adults worldwide have been exposed to HCMV. Whereas it is typically unnoticed in healthy individuals, HCMV infection can become life-threatening for high-risk groups such as pre- and postnatal infants or immunocompromised individuals e.g HIV-infected patients and organ transplant recipients. Therefore, these individuals require regular immune monitoring to detect acute HCMV infections.

HCMV in Research

Studies in humans and animal models have demonstrated a critical role for both CD4+ and CD8+ T-cell immunity in limiting viral replication and preventing the clinical manifestations of progressive infection. Many studies focus on pp65 for general immune monitoring. However, other antigens can be immune relevant and are often investigated in HCMV vaccine development. Furthermore, HCMV specific humoral immune responses have been reported and correlated to viral load.

JPT's Peptide Tools to Study HCMV:

Cellular Immune Response Profiling

JPT offers tailored custom peptides, peptide libraries and peptide pools for HCMV research. We provide knowledgeable support, high quality, flexible and innovative formats combined with expert know-how to bring forward your research project. In addition, we offer GxP Peptides & Pools for immunotherapy and specific clinical applications at attractive pricing.

PepMix™ Peptide Pools for antigen specific stimulation of T-cells in

  • Immune monitoring of high-risk patients
  • Qualification of immunodominant antigens
  • Validating clinical T-cell assays

PepMix™ Collection HCMV
The PepMix™ Collection HCMV provides peptide pools (2 aliquots each) of 19 different antigens in one plate for fast and economic immune response profiling.

HCMV PepMix
™ Peptide Pools
Individual peptide pools comprise overlapping peptide scans through single immunodominant antigens:
IE-1, IE-2, pp65, UL28, UL32, UL36, UL40, UL48, UL55, UL82, UL86, UL94, UL99, UL103, UL151, UL153, US3, US24, US29 and US32.

Tailored HCMV PepMix™ Peptide Pools
Peptide Pools tailored for your specific needs!

> PepMix
™ Peptide Pools product Page

PepTrack™ Peptide Libraries for T-cell assays in

  • High-throughput T-cell epitope discovery
  • Monitoring of cellular immune response
  • Clinical trials

> PepTrack™ Peptide Libraries Product Page

Humoral Immune Response Profiling

Peptide Microarrays for humoral immune response profiling in

  • Immune monitoring of humoral responses
  • Profiling of HCMV specific samples or antibodies
  • Evaluation of co-infection
  • Detection of epitopes and epitope spreading

Catalog RepliTope™ Peptide Microarrays
Ready-made peptide microarrays displaying overlapping peptides through single immunodominant antigens:
IE-1, IE-2, pp65, UL28, UL32, UL36, UL40, UL48, UL55, UL82, UL86, UL94, UL99, UL103, UL151, UL153, US3, US24, US29 and US32.

RepliTope™ Antigen Collections
• The RepliTope™ Antigen Collection combines all 20 proteins above on one microarray for optimal coverage of the most important antigens in a single experiment.
• The RepliTope™ Antigen Collection HCMV (whole proteome) displays a peptide scan through the entire HCMV proteome (over 17 000 peptides) on three peptide microarrays.
> RepliTope™ Peptide Microarrays Product Page

Tailored PepStar™ Peptide Microarrays
You define content and layout, we provide economic and fast production in our regulated clean-room environment. We also offer our assay and analysis service using your samples with your tailored peptide microarray.
> Tailored PepStar™ Peptide Microarrays Product Page

Peptide ELISA

In addition to high-content peptide microarrays, JPT offers the development of peptide based enzyme-linked immunosorbent assays (ELISA). This common analytical and highly sensitive immunological assay is well established for proteins but requires detailed experimental know-how for peptides. Peptide ELISA is offered as stand alone service for mapping of epitopes and definition of protein interaction sites or as validation assay to confirm results obtained with JPT’s peptide microarrays.
> Peptide ELISA Product Page

GxP Peptides & Pools

GxP Peptides & Pools

We established an enhanced production environment for GxP peptides that goes beyond ISO 9001:2015 regulations for the more stringent product requirements of immunotherapy as well as vaccine & drug development. Thus, the resulting GxP peptides and GxP peptide pools have been approved for specific clinical applications. 

> GxP Peptides & Pools Product Page

Selected HCMV Related References

"Reconstitution of Cytomegalovirus-specific T-cell Response in Allogeneic Hematopoietic Stem Cell Recipients: the Contribution of Six Frequently Recognized, Virus-encoded ORFs"
Nemeckova et al., Transpl Infect Dis. (2016) – PMID: 27061389
"Cellular Interferon Gamma and Granzyme B Responses to Cytomegalovirus-pp65 and Influenza N1 Are Positively Associated in Elderly"
Theeten et al., Viral Immunology (2016) – PMID: 27002465
"Impact of Persistent Cytomegalovirus Infection on Dynamic Changes in Human Immune System Profile"
Vescovini et al., PlosOne (2016) – PMID: 26990192
"Cytotoxic Polyfunctionality Maturation of Cytomegalovirus-pp65-specific CD4+ and CD8+ T-cell Responses in Older Adults Positively Correlates with Response Size"
Chiu et al., Sci Rep (2016) – PMID: 26778409
"In Vitro Studies of the Impact of Maribavir on CMV-Specific Cellular Immune Responses"
Stachel et al., J Clin Virol. (2015) – PMID: 26780109
"IL-12–Dependent Cytomegalovirus-Specific CD4+ T Cell Proliferation, T-bet Induction, and Effector Multifunction during Primary Infection Are Key Determinants for Early Immune Control"
Popescu et al., J Immunol. (2015) – PMID: 26663780
"Dichotomous Effects of Latent CMV Infection on the Phenotype and Functional Properties of CD8+ T-cells and NK-cells"
Bigley et al., Cellular Immunology (2015)
"Latent CMV Infection in Rheumatoid Arthritis Increases Frequencies of cytolytic LIR‐1+ CD8+ T Cells"
Rothe et al., Arthritis Rheumatol. (2015) – PMID: 26314621
"CMVpp65 Vaccine Enhances the Antitumor Efficacy of Adoptively Transferred CD19-Redirected CMV-Specific T Cells"
Wang et al., Clin Cancer Res. (2015) – PMID: 25838392
"Discordant Humoral and Cellular Immune Responses to Cytomegalovirus (CMV) in Glioblastoma Patients Whose Tumors are Positive for CMV"
Rahbar et al., Onco Immunology (2015)

More references

 

 

Selected HCMV Related Testimonials

“I have been collaborating with JPT for several years. Their unique peptide library technologies and knowledge of specific peptide requirements for T-cell assays made them a valuable, long-termpartner in our efforts to develop novel adoptive immunotherapy approaches to prevent and treat viral infections post-transplant as well as for the treatment of virus and non virus-associated malignancies. Furthermore, their reagents allow us to precisely monitor and track specific T-cell populations post-infusion.”
Ann M. Leen, PhD, Baylor College of Medicine, Center for Cell andGene Therapy, Houston, USA.

“Over the past five years my research team has been active in the development of species-specific peptide arrays. Our ability to advance this technology into new research areas has been dependent on working with the highest quality of arrays. In this regard, JPT’s commitment to excellence has been a critical foundation for the success of our research program. I have been consistently impressed with their customer service, technical expertise and professionalism. When it comes to peptide work there are no other options for our group… JPT is the best.”
Scott Napper, PhD, Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Canada

More testimonials

News Highlights

  • Performance Control of T-cell Assays
    Check the innovative, easy method to produce cellular reference samples for antigen-specific T-cell assays (TERS).
    (Nov 2016)
  • Analyze 21 Samples on a Single Microarray with our new RepliTope™ Multiwell Microarrays available for Tumor Antigens and Infections
    (August 2016)

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Quality Assurance

All production is performed according to ISO 9001:2015 standards

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