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Peptide Tools to Study Autoimmune Diseases

About Autoimmune Diseases

  • Abnormal immune responses directed towards the body's own proteins (self-antigens)
  • Leads to tissue damage
  • 80 types of autoimmune diseases known such as systemic lupus erythematosus, celiac disease, diabetes mellitus type 1, Graves' disease, multiple sclerosis and rheumatoid arthritis
  • Causes are mostly unknown, diagnosis can be difficult
  • Treatments can improve symptoms
  • Autoimmune diseases cannot be cured

Target & Epitope Discovery

Target and epitope discovery represent major challenges to enable early diagnosis and the development of suitable treatment strategies. JPT possesses a variety of unique high-throughput peptide-based technologies enabling deep discovery of relevant targets as well as systematic discovery of B- and T-cell epitopes.

In addition, our ultra-fast and cost efficient peptide synthesis strategies together with sophisticated bioinformatics approaches enable sequence diversity considerations (splice variants, mutations etc.) and post-translational modifications that have not previously been addressed from proteome-spanning perspectives.

JPT's Peptide Tools to Study Autoimmune Diseases:

Discovery

SpotMix™ Antigen Discovery Pools

SpotMix™ Antigen Discovery Pools consist of overlapping & pooled peptides synthesized by our proprietary ultra-high throughput SPOT technology. SPOT peptides are synthesized in small amounts fully automated, quickly, at low cost and then pooled to span an entire antigen. SpotMix™ Pools are optimal for broad antigen target discovery.

> SpotMix™ Antigen Discovery Pools Product Page

PepTrack™ Peptide Libraries for T-Cell Assays

Our customized peptide libraries with flexible layout and specifications are optimized for the use in T-cell assays such us ELISPOT and intracellular cytokine staining. Applications range from epitope discovery and validation to the development of epitope based cellular therapy concepts.

> PepTrack™ Peptide Libraries Product Page

Matrix Peptide Pools & PepMixes™

PepMix™ purified peptide pools spanning entire antigens are highly efficient tools used to validate immune relevant antigens for research, immune monitoring and development of immune therapy concepts. Our custom designed 2D and 3D matrix pools enable antigen spanning T-cell epitope discovery saving time and precious patient samples. The validated pooling strategy ensures the presence of all peptides in a pool with guaranteed purity for each peptide.

> PepMix™ Peptide Pools Product Page

PepStar™ Peptide Microarrays

Our customized peptide microarrays for profiling of patient samples or antibodies display overlapping peptide scans through antigens or antigen collections. You define content and layout, we provide economic and fast production in our regulated clean-room environment. We also offer a comprehensive assay and analysis service for your samples!

> PepStar™ Peptide Microarrays Product Page

SpikeMix™ – Peptide Standards for Mass Spectrometry based Proteomics

JPT offers pools of proteotypic peptides for the detection and relative quantification of relevant antigens and immunodominant epitopes. The peptide pools incorporate stable isotope labeled peptides for use in mass spectrometry-based MRM assays.

> SpikeMix™ Product Page

Cellular Immune Response

Monitoring of Immune Responses

JPT’s immune monitoring tools for cellular immune response target specific antigens or antigen families. JPT’s unique combination of technical and bioinformatics knowledge provides efficient epitope resolved profiling of cellular immune responses that can be correlated to clinical data. 

PepMix™ Peptide Pools

Our widely used PepMixes™ are available in a large variety of off-the-shelf peptide pools or tailored to your specific application and antigen. They contain peptides of defined purity and amount. We also offer a wide variety of standardization and control pools such as CEFX/ EFX Pools and HCMV (pp65).

> PepMix™ Peptide Pools Product Page

MHC Multimers for Detection of Antigen-Specific T cells

MHC Multimers are used for the detection, enumeration and isolation of antigen-specific T cells in fluid cell samples (PBMC, blood) by flow cytometry.

> MHC Multimers Product Page

Humoral Immune Response

Humoral Immune Response

Our humoral immune monitoring tools and services for high resolution profiling of target specific antigens or antigen families. A combination of technical and bioinformatics knowledge provides efficient epitope resolved profiling.

PepSpot™ Peptide Arrays on Cellulose

PepSpots™ are membrane bound peptide arrays (PepSpots™) for efficient and inexpensive antibody epitope mapping or protein-protein interaction studies. PepSpot™ is the method of choice for fast and reliable antibody epitope mapping and characterization using purified antibodies.

> PepSpots™ Peptide Arrays Product Page

PepStar™ Peptide Microarrays

Our customized peptide microarrays for immune profiling and monitoring display overlapping peptide scans through antigens or selected amino acid sequences. You define content and layout; we provide economical and rapid production in our regulated clean-room environment. We also offer assay and analysis services for your samples with a tailored peptide microarray.

> PepStar™ Peptide Microarrays Product Page

Selected References

"Quantitative Proteomics Suggests Decrease in the Secretogranin‐1 Cerebrospinal Fluid Levels During the Disease Course of Multiple Sclerosis"
Kroksveen et al, Proteomics (2015)
"High-Density Peptide Microarray Analysis of IgG Autoantibody Reactivities in Serum and Cerebrospinal Fluid of Multiple Sclerosis Patients"
Hecker et al, Mol Cell Proteomics (2016)
"Antigen Arrays for Profiling Autoantibody Repertoires"
Ayoglu et al, Bioanalysis (2016)

More references

Selected Testimonial

"The focus of the Clinical Immunology Group at the German Rheumatism Research Centre is to study the role of cells and molecules in the origin and progression of autoimmune and other diseases. In our hands PepMixes™ were found to be effective in monitoring the immune status of various patient populations but show also promise for the development of novel immuno therapy approaches."
Dr. Andreas Thiel (German Rheumatism Research Centre, Clinical Immunology, Berlin, Germany)

More testimonials

 

 

Selected Application Notes

Multiple Sclerosis and Epstein‐Barr Virus Infection An Epitope Mapping Study
by Reimer et al - Application Note  (2015)

More application notes

 

 

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Quality Assurance

All production is performed according to ISO 9001:2015 standards

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